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Showing 1 to 20 of 288 for “"Stationary phase"”.
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Studies on stationary phase Vibrio sp. 2
Vibrio sp. 2 stationary phase cells are novel and interesting in that they are able to support phage growth in standing cultures, but not in shaken (aerated) cultures. Many physiological and morphological characteristics change when Vibrio sp. 2 stationary phase cells are removed from aeration: the …
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Novel Switchable Stationary Phase Methods For Supercritical Fluid Chromatography
… (SFC-FID) system employing a modified water stationary phase was investigated for the analysis of organic bases. The method uses a stainless steel capillary column coated with water containing NH4OH, which was also used to hydrate the system. In this way, the pH of the water stationary phase …
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Separation, Enantiorecognition, and Chiral Stationary Phase Design of Organophosphonates
Mechanistic investigations of several chiral stationary phases (CSPs) were undertaken. These investigations were carried out in an effort to understand the recognition processes responsible for the observed enantioselectivities on the CSPs of interest. Several spectroscopic and chromatographic …
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Spectroscopic Study of Compressible Mobile Phase and Stationary Phase Behavior in Chromatography
… investigate the behavior of compressible mobile phases and stationary phases under a variety of chromatographic conditions. Efforts to understand and optimize separations employing compressible mobile phases have been limited by a lack of understanding of the mobile phase density gradient. Mobile …
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Direct High Pressure Liquid-Chromatographic Separation of Enantiomers Using a Chiral Stationary Phase
Made available in DSpace on 2014-12-13T20:10:32Z (GMT). No. of bitstreams: 1 7913489.pdf: 4055959 bytes, checksum: 1b59cd26023407fa8f92d0edc55d48a3 (MD5) Previous issue date: 1978
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Enantio-Selective Mechanism of the Poly-Proline Chiral Stationary Phase: a Molecular Dynamics Study
Poly-proline-based chiral stationary phases are relatively new stationary phases and have shown to be competitive to other commercially available chiral stationary phases for high performance liquid chromatography (HPLC). The conformational studies, solvation properties and enantio-selective …
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Gene transfer agents promote survival and DNA repair during stationary phase for Caulobacter crescentus
… that GTAs promote the survival of Caulobacter in stationary phase and following DNA damage by providing recipient cells a template for homologous recombination-based repair. This function may be broadly conserved in other GTA-producing organisms and explain the prevalence of this unusual …
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Polyploidy in Saccharomyces cerevisiae leads to the loss of cell cycle control in stationary phase
… patterns of haploid and tetraploid cells during stationary phase reveals that tetraploids are defective for growth arrest during nutrient deprivation.
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The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase
… increase in binding to chromatin during stationary phase. Because of the role of H1 in gene expression and chromatin compaction, it is essential to understand the mechanism behind this change in binding behaviour for a complete mechanistic description of gene regulation. We postulated …
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An Evaluation Of UHPLC-MS Technology Encompassing Elevated Temperatures, Low Viscosity Operation and Recently Developed Stationary Phase Materials
There are several approaches to reducing mobile phase viscosity in order to overcome high pressures related to stationary phase permeability. This can be achieved either by elevating column temperature and or by employing organic rich eluents. To begin with, a standard application of UHPLC-MS was …
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Development of stationary phase materials and methods for analysis of pharmaceuticals via high-performance liquid chromatography and electrochemical detection
… novel polymer immobilised porous silica particle phase for fast LC analysis of selected pharmaceutical actives. In this thesis, a Nafion polymer-coated silica stationary phase material for the fast and efficient separation of pharmaceutical solutes is reported. Initially, quaternary amine …
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Synthesis & retention mechanisms of novel mixed mode stationary phase for detection of specific polar low molecular weight biological samples
… and application of novel mixed mode stationary phases for Hydrophilic Interaction Liquid Chromatography (HILIC). HILIC is a rapidly emerging chromatographic mode that is finding great applicability in the analysis of polar organic molecules. In addition, there is a chapter on the …
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Compaction of chromatin in Saccharomyces cerevisiae
… and the compaction of the yeast genome during stationary phase. The relative gene content of condensed chromatin, fractionated and isolated by sucrose gradient ultracentrifugation from stationary and exponential phase cultures was compared using genome-wide technologies. This study showed that …
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High level indole signalling in Escherichia coli
… found in an E. coli culture supernatant in stationary phase (typically 0.3-0.8 mM). Recently, it has been shown that much higher concentrations of indole (3-5 mM) inhibit cell division by acting as an ionophore to dissipate the membrane potential. However the biological relevance of such …
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