Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Stat1 phosphorylation"”.
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Regulation of STAT-1 Phosphorylation by Histamine Through H1 and H2 Receptors: Inverse Agonism and PKA-PKC Interaction in STAT-1 Phosphorylation
… transducer and activator of transcription-1 (STAT1) is a latent signal transducer protein which, on phosphorylation, is translocated from the cytoplasm to the nucleus and is subsequently activated. This work was designed to determine the role of histamine in the regulation of ST ATI …
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DJ-1, a Parkinson’s Disease-associated Gene, Prevent Excessive Glial Inflammatory Responses through Interaction with SHP-1
… by facilitating the interaction between STAT1 and its phosphatase, SHP-1 (Src-homology 2-domain containing protein tyrosine phosphatase-1). Astrocytes and microglia cultured from DJ-1-knockout (KO) mice exhibited increased expression of inflammatory mediators and phosphorylation levels of …
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Identifying CDK8/19 as a Novel Regulator of EWS-FLI1 Function in Ewing Sarcoma
… I utilized a previously described IFN-STAT1 phosphorylation assay used to test CDK8/19 activity. I identified a novel, potent small molecule capable of interrupting CDK8/19 activity. Through biochemical reconstitution of the full CDK8 module, I determined our novel CDK8/19 inhibitor is …
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Unravelling the epigenetic landscape of pancreatic cancer: The role of cancer-associated fibroblasts in transcription factor regulation
… and proteomic approaches. This work identified STAT1 as a mediator of iCAF-induced transcriptional reprogramming. STAT1 was found to associate with accessible chromatin regions and to co-occupy a subset of sites with FOXA1/FOXA2, promoting expression of interferon (IFN)-α/γ response genes. …
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Modulation of innate immune signaling pathways by the intracellular pathogen Toxoplasma gondii
… Toxoplasma activates NF-[kappa]B and inhibits STAT1 signaling pathways to promote both its own survival and the survival of its host. We identified GRA15, a novel Toxoplasma secreted factor that activates the host cell NF-[kappa]B pathway. GRA15 is polymorphic between Toxoplasma strains and …
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HDAC6 Deletion Decreases Pristane-Induced Inflammation and Lupus
… increased acetylation of NF-κB while increasing STAT1-phosphorylation which caused the synthesis of inducible nitric oxide synthase (iNOS) in cells activated by LPS and IFN-γ. Long-term pristane treatment induced proteinuria in female mice although there were no significant differences between WT …
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The intrinsic role of SOCS1 in intestinal epithelial cells
… in colitis Socs1ΔIEC mice seems driven by STAT1-dependent sensitization of Socs1- null IECs to apoptosis induced by immune cells-derived IFNγ in response to bacterial invasion. To define SOCS1 IEC-intrinsic role in the epithelium integrity and renewal, and for confirming that SOCS1 …
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Genomic investigation of primary immunodeficiency
… transducer and activator of transcription 1 (STAT1) phosphorylation. Also, due the reduction in the E3 ligase action of SOCS1, patient cells showed increased Toll-like receptor cytokine responses and hyperactivity of the Akt pathway. Thus, dysregulation of multiple immune pathways leads to …
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Analysis of Human Interferon Signaling and its Regulation
… the cell surface, which induces an intracellular phosphorylation-dependent signaling cascade. The active Janus kinases JAK1 and tyrosine kinase 2 (TYK2) phosphorylate signal transducer and activator of transcription 1 (STAT1) and STAT2, which act as a transcription factor together with …
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Cytokine resistance in melanoma
… it has been reported that methylation of STAT1 on a conserved arginine residue (Arg31) is important for STAT1 function and loss of this modification was proposed to be involved in interferon resistance of cancer cells. Since STAT3 is involved in growth inhibition of melanoma cells, in the …
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Bedeutung von Interleukin-6, Oncostatin M und Interleukin-31 für die biochemische und immunologische Barrierefunktion der Haut
… Both cytokines induced a strong activation of STAT1 and STAT3 (signal transducer and activator of transcription) as well as the MAPK (mitogen-activated protein kinase) Erk1/2. In contrast to IL-6, OSM additionally activated protein kinase B/Akt and the stress-activated MAPK p38. While the …
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Inactivation of the JAK-STAT Pathway
… cells the appearance of tyrosine phosphorylated Stat1 in the nucleus was maximal within 20-30 minutes, remained for 2-2.5 hours and activated molecules disappeared by 4 hours. In the absence of continued signaling from the IFN-γ receptor (imposed by staurosporine treatment) previously activated …