Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Stall Force"”.
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Force studies of intracellular transport
… which allows us to calibrate and measure forces in a living cell and other viscoelastic environments. We have found that lipid droplets in A549 cells and phagocytosed beads in Dictyostelium cells typically have 1 active plus-end directed motor and 1 active minus-end directed motor. Also, …
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Kinesin's force generation mechanism : study and practical application
… 5 (Eg5) constructs were used to study the force generation mechanism of the motor protein. The kinesin family of proteins walk along microtubules to carry cargo or pull microtubules along each other and do so by hydrolyzing a single ATP per 8nm step. The mechanism that is employed by …
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Experimental and theoretical investigation of mechanism of Kinesin motility
… from ATP hydrolysis to generate mechanical force to power its progressive motility along a microtubule track. The mechanism of motility has been a subject of extensive study for last decade. Recently, it has been proposed that novel element-cover strand-is essential in power-stroke-like …
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Studies of Huntington's disease associated motor domain phosphorylation of kinesin-1
… phosphorylation or mutation led to a decreased stall force. Furthermore, polystyrene bead cargos coated with dynein and kinesin traveled preferentially in the minus direction when residue 175 of kinesin was negatively charged. These results show that modification of serine 175 alone is …
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Structure and mechanics of the spasmoneme, a biological spring within the protozoan Vorticella convallaria
… work the maximum load at minimum velocity (the stall force), is measured. Work done by the stalk in contraction is shown to be dependent on its fractional change in length. This energy dependence arises from the basic underlying mechanism, and a major goal of this thesis was to characterize that …
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An investigation of the mechanics of an actin spring
… or myosin-driven processes. The motive force for this extension originates from stored elastic energy in the overtwisted, pre-formed coilmuch like a compressed mechanical spring. When the actin bundle untwists, this energy is converted to mechanical work powering the extension. We …
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Motor Design and Control for Scalable Distributed Actuation
… resolution over 45mm of stroke, with a 1.9N stall force, and a density of 104 actuators per square foot. Motor commutation is achieved through multiplexing of individual motor windings, allowing for sub-linear cost and component count scaling. Actuator performance over a number of performance …
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Tools to study the kinesin mechanome using optical tweezers
… kinesin walks on a microtubule and generates force. Recent MD simulations have identified the force-generating mechanism in kinesin, the cover-neck bundle, and strongly suggest that the formation of the CNB by the N-terminal cover strand and the C-terminal neck linker of the motor head are …
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Chemomechanical coupling and motor cycles of the molecular motor myosin V
… such as nucleotide concentration and applied forces. In addition, numerous kinetic rates involved in the enzymatic reaction of the molecule have been determined. For forces that exceed the stall force of the motor, myosin V exhibits a 'ratcheting' behaviour: For loads in the direction of …