Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"Stable isotope labeling"”.
-
Comprehensive analysis of metabolic pathways through the combined use of multiple isotopic tracers
… important limitation of MFA, as carried out via stable isotope labeling and GC/MS measurements, is the large number of isotopomer equations that need to be solved. This restriction reduces the ability of MFA to fully utilize the power of multiple isotopic tracers in elucidating the physiology of …
-
Quantitative analysis of carbon fluxes for fat biosynthesis in wild-type and IRS-1 knockout brown adipocytes
… (IRS-1 KO) brown adipocytes were analyzed by stable-isotope labeling, GC/MS, and flux estimation. Glutamine contributed more to fatty acid synthesis than glucose in WT cells while glucose's contribution was heavier in IRS-1 KO cells. Unlike the straightforward pathway for lipogenesis from …
-
Identification of a novel metastasis enhancer, CDCP1, and analysis of its functions during melanoma metastasis
… versus low metastatic abilities. Using SILAC (stable isotope labeling with amino acids in culture) coupled with nano-spray tandem mass spectrometry, this work led to the discovery of C̲ub Ḏomain C̲ontaining Protein 1 (CDCP1) as one of those differentially expressed transmembrane proteins. We …
-
Differential expression profiling of proteomes of pathogenic and commensal strains of Staphylococcus aureus using SILAC
… (MS)-based proteomic approach, known as stable isotope labeling of amino acids in cell culture (SILAC). Four commensal and pathogenic strains each were grown in the SILAC minimal media (RPMI 1640), containing light (12C) and heavy (13C) form of lysine, respectively, until early stationary …
-
Study of the differential compartmentation and functions of hexokinase isoforms and implications for cancer metabolism
… of HK for cell growth and proliferation using stable isotope labeling and metabolic flux analysis. Key findings were verified in a hepatocellular carcinoma cell line using low glucose feeding or HK inhibitor. These experiments identified differential HK enzymatic activity in different cell …
-
Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
… peptide segments of these proteins. SILAC (Stable Isotope Labeling by Amino acids in Cell Culture) based CWP quantification analyses were performed to monitor CWP accumulation level change in response to hyphae induction. Information on surface exposed peptide segments and regulation of cell …
-
DEVELOPMENT OF SINGLE-CELL MASS SPECTROMETRY TOOLS TO INVESTIGATE METABOLIC REORGANIZATION DURING EARLY EMBRYOGENESIS
… coverage of metabolism. Chapter 4 discusses a stable-isotope labeling strategy and single-cell CE-MS to uncover metabolic pathways involved in cell differentiation. Chapter 5 details the application of our custom-built microprobe sampling technique to investigate spatial cell heterogeneity in …
-
Proteomic Analysis of Plasma Membrane Proteins from Drug Susceptible and Drug Resistant Breast Cancer Cell Lines
… is then used in combination with metabolic stable isotope labeling to identify protein expression changes between the mitoxantrone drug susceptible and drug resistant MCF-7 breast cancer cell lines.
-
The Processing of Amyloid Precursor Protein in the Central Nervous System of Humans and Rhesus Macaques
… from CSF of participants undergoing stable isotope labeling kinetics studies. Isolated sAPP underwent proteolytic cleavage by trypsin and turnover rates of total sAPP were determined by LC-MS and compared to turnover rates of Aβ. Isolated total sAPP that underwent proteolytic cleavage …
-
IQGAP1 is a novel effector of gonadotropin-releasing hormone receptor signaling
… containing low-density plasma membranes. SILAC (stable isotope labeling by amino acids in cell culture) in combination with mass spectrometry demonstrated that GnRH, within 10 min, altered the association of 87 proteins with this plasma membrane fraction. Ontology analysis revealed that GnRH …
-
COMPARATIVE PROTEOMICS STUDIES OF SOLUBLE NUCLEAR PROTEINS OF DRUG SUSCEPTIBLE AND RESISTANT HUMAN BREAST CANCER MCF-7 CELLS
… cells. Two dimensional gel electrophoresis and stable isotope labeling by amino acids in cell culture are used to conduct the study. Protein identification is acquired by peptide fingerprinting or microsequencing. Relative quantitation of the proteins is derived from gel comparisons and from …
-
Sustainable Nanomaterials Combined with Raman Spectroscopy-based Techniques to Advance Environmental Sensing
… The second application presents a simple Raman-stable isotope labeling approach for the study of viral infection of bacteria. The third application involved use of SERS pH nanoprobes for measuring pH in droplets of complex matrices (e.g., DMEM cell culture media, human saliva).
-
Modulating Amyloid Beta Clearance by Altering Cellular and Whole Brain Apolipoprotein E Metabolism
… brain, we developed a technique that couples stable isotope-labeling kinetics (SILK) with mass spectrometry. We validated this technique by demonstrating that apoE clearance is enhanced in the brains of mice overexpressing LDLR. We also applied this technique to measure apoE clearance rates in …
-
Characterizing Modified Nucleosides in RNA by LC/UV/MS
… from tRNA and rRNA, without resorting to stable isotope labeling methods, we are now able to define the statistical significance of changes in modification status when comparing biological systems of varying environmental or physiological cellular conditions, which should enable future …
-
Protein Set for Normalization of Quantitative Mass Spectrometry Data
… most popular method for quantitative proteomics. Stable isotope labeling and label-free quantitation methods are widely used in quantitative mass spectrometry experiments. Proteins with stable expression level and key roles in basic cellular functions such as actin, tubulin and …
-
Understanding the Molecular Basis for FGF15/19 and FGF21 Actions on Energy Homeostasis
… substrates affected by this hormone. Utilizing stable isotope labeling of amino acids in cell culture (SILAC), an unbiased phosphoproteomic profile was obtained of potential FGF21 targets in rat H4IIE hepatoma cells. One of the most highly regulated targets was FetuinA, which was …
-
Quantifying ubiquitin dynamics
… conjugation pathways that we call SILOW or Stable Isotope Labeling with ¹⁸O-Water. Our method is applicable to both yeast and mammalian cells, does not perturb cellular physiology in any way and can be used with conventional proteomics methods. SILOW permits rapid changes in Ub flux to be …
-
Role of O-GlcNAc in the Vertebrate Secretory Pathway
… response to stimuli. Our approach utilizes both stable isotope labeling with amino acids in cell culture (SILAC) for quantitation and metabolic labeling of O-GlcNAc for enrichment. Using our glycoproteomics workflow we examined the effects of brefeldin A (BFA), a fungal metabolite that disrupts …
-
Development and Application of a quantitative Mass spectrometry based Platform for Thermodynamic Analysis of Protein interaction Networks
… thermodynamic analyses that involves the use of Stable Isotope Labeling by Amino acid in cell Culture (SILAC) quantitation. A solution-based SILAC-SPROX protocol is described in Part III and a SILAC-SPROX protocol involving the use of cyanogen bromide and a gel-based fractionation step is …
-
Insights into the molecular mechanisms of function and regulation of histone crotonylation
… using peptide pull-downs combined with stable isotope labeling with amino acids in cells in culture (SILAC). This was complemented by a proteome array screen for direct crotonylation interactors. These two independent assays identified the histone chaperone DAXX, an important chromatin …
Page 1 of 2