Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Stable Transformation"”.
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Microprojectile-mediated transformation of Ornithogalum thyrsoides hybrid A2
… study investigated the feasibility of biolistic transformation for Ornithogalum. Two PDS 1000/He biolistic guns were used in independent studies, one in 1995 at the Floral and Nursery Plants Research Unit of the National Arboretum of the United States Department of Agriculture (USDA) and the …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… of optimal conditions for a genetic transformation system for tall fescue, several factors influencing transient gene expression were studied in protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene …
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The Evolution and Development of Nectar Spurs
… in *L. vulgaris*. Protocols for VIGS and stable transformation were trialled and developed for use in *L. vulgaris*, marking the first successful attempts to transform this species. In addition to exploring genes hypothesised to play a role in spur *initiation*, candidate genes controlling …
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Aggregation Pheromone Biosynthesis and Engineering in Plants for Stinkbug Pest Management
… benthamiana leaves. Future work will implement stable transformation to engineer murgantiol biosynthesis in crucifer trap crops and develop similar approaches for pheromone engineering of other agricultural pests.
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The modification of Anthocyanin biosynthesis in dicotyledonous plants
… of the genes following both transient and stable transformation experiments in plants. A novel method was devised to overcome the problems associated with the insertion of multiple chimeric genes into a binary transformation vector. A total of fourteen intermediate expression plasmids and …
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The modification of Anthocyanin biosynthesis in dicotyledonous plants
… of the genes following both transient and stable transformation experiments in plants. A novel method was devised to overcome the problems associated with the insertion of multiple chimeric genes into a binary transformation vector. A total of fourteen intermediate expression plasmids and …
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BIOSYNTHESIS AND FUNCTION OF CARDIAC GLYCOSIDES IN THE CRUCIFER GENUS <i>ERYSIMUM</i>
… genome assembly and a protocol for floral dip stable transformation. In subsequent chapters, I use metabolomic and transcriptomic datasets to identify candidate genes for cardiac glycoside biosynthesis and test candidate gene function using CRISPR/Cas9-mediated gene editing, in vitro assays …
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Agrobacterium-mediated gene transfer into kiwifruit
… in the establishment of Agrobacterium-mediated transformation for new plant species. A series of binary vectors have been constructed that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from transformed plant cells to …
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Toward developing pheromone emitting trap crops: Metabolic engineering of an aggregation pheromone for enhanced attraction of Phyllotreta cruciferae
… synthase (PsTPS1) to himachaladiene. Transient transformation of N. benthamiana with PsIDS3-TPS1 co-localized to the plastid resulted in the emission of himachaladiene and other known PsTPS1 products. Daily emissions of himachaladiene were approximately 1 µg per plant, which is six-fold higher …
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Development of genetic transformation systems in creeping bentgrass (Agrostis palustris Huds.)
… this study was conducted to develop genetic transformation systems in creeping bentgrass. Establishment of embryogenic cell cultures is a prerequisite for crop improvement via genetic engineering. A protocol for initiating and maintaining embryogenic callus and suspension cultures in creeping …
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Functional genomics of nodulins in the model legume Lotus japonicus
… analysis of candidate gene expression. As stable transformation of Lotus japonicus takes several months, an Agrobacterium rhizogenes transformation system was established in the lab and growth conditions for screening transformants for symbiotic phenotypes were improved. These platforms …
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Regulation of anthocyanin accumulation in apple by the transcription factor MdMYB10
… and highly pigmented apple plants following stable transformation with constitutively expressed MdMYB10. Efficient induction of anthocyanin biosynthesis in transient assays by MdMYB10 was dependent on the co-expression of two bHLH proteins from apple, MdbHLH3 and MdbHLH33. A strong …
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… a plasmid construct was identified that lead to stable transformation of Leishmania when electroporated into promastigotes as an intact circular plasmid. This construct conferred selectable drug-resistance to transfectants and was found to replicate as an extrachromosomal circular concatamer. The …
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Developing molecular genetic tools for gene discovery in soybean (Glycine max)
… plants, obtained using the cotyledonary node transformation method, harbored induced mutations in either or both GmFAD2 genes. Importantly, 40% of the T0 plants harbored homozygous or biallelic mutations in both genes and these mutations were heritable in T1 and T2 progenies. Seeds derived …