Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Stable Isotope Probing"”.
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Stable isotope probing of hyperthermophilic filamentous microbial communities in Octopus Spring, Yellowstone National Park
Stabe isotope probe (SIP) incubation studies were performed using ¹³C-labeled carbon substrates on hyperthermophilic filamentous streamer communities inhabiting Octopus Spring in Yellowstone National Park. Biomass was removed from the outflow stream and incubated at near-in situ conditions with …
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Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° North using 13C DNA Stable Isotope Probing and Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization
… catalyzing them, this research used DNA Stable Isotope Probing (SIP) combined with Catalyzed Activated Reporter Deposition‐Fluorescence in situ Hybridization (CARD‐FISH) to identify the microorganisms chemoautotrophically supporting the food web at a diffuse flow hydrothermal vent. Both …
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Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° north using ¹³C DNA stable isotope probing and catalyzed activated reporter deposition-fluorescence in situ hybridization
… catalyzing them, this research used DNA Stable Isotope Probing (SIP) combined with Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) to identify the microorganisms chemoautotrophically supporting the food web at a diffuse flow hydrothermal vent. Both …
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Bioreactor Janssand : fermentation processes in tidal-flat sediments of the German North Sea
… zur Aufklärung der Fermentationsprozesse und „stable-isotope probing“ (SIP) zur Identifizierung der aktiv substratassimilierenden Organismen miteinander kombiniert wurden. Es wurden Abbauversuche mit drei verschiedenen Modellsubstraten durchgeführt. Im ersten Teil dieser Studie wurde …
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Analogue enrichment of aromatic degradation through application of lignin-derived phenols
… metagenomic procedures _ primarily based on DNA stable isotope probing, coupled with Illumina sequencing of labelled DNA, and bioinformatics analysis (Kaiju, Hmmer and Krona). Naturally occurring lignin-derived phenols (vanillin) are found to have influence on the phylogenetic and functional …
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Development of Integrated Photobioelectrochemical System (IPB): Processes, Modeling and Applications
… Chapters 4 and 5 demonstrate the application of stable isotope probing to study Shewanella oneidensis MR-1 in the MFCs. Chapters 6 to 8 describe the application of models to optimize MFC and IPB system performance. Chapter 9 describes the strategy improvement for the algal harvesting in IPB. …
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Impact of Oxygen and Pesticides on Microbial Cellulose Degradation in Aerated Agricultural Soils: A Microscaled Analysis of Processes and Prokaryotic Populations
… [13C]-labeled saccharides by 16S rRNA stable isotope probing. The metabolic response of major bacterial taxa to pesticides and fluctuating availabilities of O2 was further analyzed with taxon-specific qPCR assays. Eukaryotes that contributed to soil carbon flux were identified by …
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Microbial communities, activities, and metabolisms in Earth’s subseafloor and analog oceanic environments
… environments. The first part of the thesis uses stable isotope probing to determine the activity of subseafloor carbon-cycling microorganisms. Chapter 2 examines chemolithoautotrophs at Axial Seamount, demonstrating that variations in hydrogen and oxygen availability strongly influence the …
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Legacy effects of temperature alterations on microbial resistance and resilience to drying and rewetting
… time, I measured bacterial and fungal growth via stable isotope probing as well as respiration via gas chromatography. I found that while moisture was affected, microbial resistance was unaffected by warming treatments. However, resilience was affected by warming treatments. Responses also …
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Isoprene Degradation in the Terrestrial Environment
… evidence of direct isoprene degradation using stable isotope probing, and the relatability to the natural environment using low isoprene concentrations. Different bacteria were enriched at different levels of isoprene, with genera such as Rhodococcus being enriched at high concentrations, and …
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METHYLATED AMINE-UTILISING BACTERIA AND MICROBIAL NITROGEN CYCLING IN MOVILE CAVE
… was investigated using a combination of DNA stable isotope probing and cultivation studies. Both, well-characterised and novel methylotrophs were identified: Methylotenera mobilis dominated 13C-monomethylamine SIP enrichments, while members of Catellibacterium, Cupriavidus and …
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Bacterial Degradation of Isoprene in the Terrestrial Environment
… environment was investigated using DNA-stable isotope probing experiments combined with 454 pyrosequencing.
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Molecular ecology of marine isoprene degradation
… for the oxidation of isoprene and propane. DNA-Stable Isotope Probing revealed that members of the genera Rhodococcus, Mycobacterium, Gordonia and Microbacterium are active isoprene degraders in the Colne Estuary.
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Recovering biomethane from fats, oils, and greases: Examining the impacts of microbial ecology on anaerobic codigester stability and bioconversion kinetics
… of selected Syntrophomonas taxa. DNA-stable isotope probing (SIP) based metagenomics confirmed that different LCFA-degrading syntrophic bacteria were selected with different codigester LCFA feeding frequencies. Taken together, the results of this study demonstrate that higher …
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The creation of novel methodologies to examine the effects of phosphorus amendments on petroleum hydrocarbon bioremediation
… investigations. The first experiment created a stable isotope probing method using labelled (18O)-phosphate to monitor soil P dynamics. Soil received either unlabelled or labelled phosphate to monitor P transformations via sequential fractionation pools over six weeks. Since the unlabelled …
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Bioavailability of metolachlor and glyphosate in aerobic and anaerobic soils
… under such soil environmental conditions. Stable isotope probing (SIP) facilitated the identification of microorganisms responsible for the mineralization of metolachlor in aerobic and anaerobic soils. The 16S rRNA gene sequences of clones implied the role of organisms closely related to …
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Mechanisms of terrestrial organic carbon export and preservation in the marine environment
… These analyses utilize the radiocarbon ages and stable carbon-13 isotopic composition of lipids produced by Archaea and Bacteria. We identify that ca. 75% of these biomarkers experience millennial scale storage in the G-B basin, in agreement with previously assessed plant-derived compounds, …
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Mechanisms of terrestrial organic carbon export and preservation in the marine environment
… These analyses utilize the radiocarbon ages and stable carbon-13 isotopic composition of lipids produced by Archaea and Bacteria. We identify that ca. 75% of these biomarkers experience millennial scale storage in the G-B basin, in agreement with previously assessed plant-derived compounds, …
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Struktur und Funktion von Fermentierern und Methanogenen in einem sauren Niedermoor
… wurden durch Anreicherung, Isolierung, 16S rRNA stable isotope probing (SIP) und 16S rRNA Genanalysen (Denaturierende Gradienten Gelelektrophorese [DGGE], Terminale- Restriktionsfragmentlängenpolymorphismus [T-RFLP]- Analyse und Genbibliotheken) abgeschätzt. Säure-tolerante Enterobacteriaceae-, …
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Structural and Functional Microbial Ecology of Denitrifying Bacteria Using Different Organic Carbon Sources
… universalis FAM5. First, the technique of DNA stable isotope probing and quantitative polymerase chain reaction were adapted to diagnose and track methylotrophic denitrifying bacteria in activated sludge. Methanol assimilating populations in the methanol fed denitrifying sequencing batch …
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