Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"SpyTag/SpyCatcher"”.
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Investigation of particulate HIV-1 Env vaccine candidates using Zera® and SpyTag/SpyCatcher technologies
… second approach investigated the two-component SpyCatcher/SpyTag technology. The stable HEK293 cell line expressing CAP256 gp140-SpyTag (gp140-ST) was generated, and trimers were purified to homogeneity using gel filtration. SpyCatcher (SC)-AP205 VLPs were produced in E. coli and purified by …
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Development of plant-produced African horse sickness vaccines
… part of the study, the use of the so-called SpyTag/SpyCatcher or bacterial “superglue” technology was investigated. This technology is based on the peptide SpyTag irreversibly coupling to the SpyCatcher protein, forming an isopeptide bond when the two are mixed together. The plant-based …
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Engineering functional biomaterials to advance environmental and resource sustainability
… a more sustainable Earth. First, we employed SpyTag-SpyCatcher chemistry to conjugate model proteins onto magnetic nanoparticles (MNPs), establishing a modular, magnetic-responsive platform for protein immobilization. This approach significantly enhanced protein stability while retaining …
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Precision Cytokine Therapy: Engineering and Delivery of CD8- and CAR-Tropic Interleukin-2 Variants
… The most promising constructs employed the SpyTag/SpyCatcher protein coupling system. In vitro experiments showed that SpyTagged-IL2 significantly enhanced phospho-STAT5 levels in SpyCatcher-expressing T cells, providing proof-of-concept that IL2 can be precisely targeted to engineered T …
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Single-Payload Bioconjugation to Native Antibodies And A General Method for the Self-Immolative Release of Amide-Containing Molecules
… This enabled the creation of protein catcher SpyTag, cytotoxic MMAE, and fluorophore BODIPY single-payload conjugates of trastuzumab. Flow cytometry and SpyTag-SpyCatcher studies carried out by Claudia Driscoll, Howarth group, showed that the ‘all-at-once’ TetraDVP concept can be used to make …
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Investigating the production of a particulate plant-produced vaccine candidate against African horse sickness
… aid antigen display, studies have utilized the SpyTag (ST) - SpyCatcher (SC) or “plug-and-display” system, a novel conjugation system used to display several antigens fused to AP205 VLPs. This study aimed at displaying the neutralizing epitope of AHSV serotype 5, known as the VP2 domain (dom) …
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Engineering outer-membrane electron transfer proteins for modular biotic-abiotic interfacing with living cells
The electrogenic bacterium Shewanella oneidensis utilises the MtrCAB protein complex to transfer electrons across the outer membrane, forming a biotic-abiotic interface that can be harnessed for energy generation, biosensing, microbial electrosynthesis and cell signalling. Within this complex, the …