Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"SpyCatcher"”.
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Investigation of particulate HIV-1 Env vaccine candidates using Zera® and SpyTag/SpyCatcher technologies
… second approach investigated the two-component SpyCatcher/SpyTag technology. The stable HEK293 cell line expressing CAP256 gp140-SpyTag (gp140-ST) was generated, and trimers were purified to homogeneity using gel filtration. SpyCatcher (SC)-AP205 VLPs were produced in E. coli and purified by …
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Development of plant-produced African horse sickness vaccines
… of the study, the use of the so-called SpyTag/SpyCatcher or bacterial “superglue” technology was investigated. This technology is based on the peptide SpyTag irreversibly coupling to the SpyCatcher protein, forming an isopeptide bond when the two are mixed together. The plant-based expression …
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Investigating the production of a particulate plant-produced vaccine candidate against African horse sickness
… display, studies have utilized the SpyTag (ST) - SpyCatcher (SC) or “plug-and-display” system, a novel conjugation system used to display several antigens fused to AP205 VLPs. This study aimed at displaying the neutralizing epitope of AHSV serotype 5, known as the VP2 domain (dom) (873bp), on …
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Precision Cytokine Therapy: Engineering and Delivery of CD8- and CAR-Tropic Interleukin-2 Variants
… most promising constructs employed the SpyTag/SpyCatcher protein coupling system. In vitro experiments showed that SpyTagged-IL2 significantly enhanced phospho-STAT5 levels in SpyCatcher-expressing T cells, providing proof-of-concept that IL2 can be precisely targeted to engineered T cell …
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Tailoring Microenvironment and Orientation of Immobilized Lactase for Improved Catalysis at Suboptimal pH
… enzyme immobilization supports. A site-specific spycatcher/spytag system was employed for direct immobilization of lactase, while polycationic polymers were incorporated to modify the local chemical environment. Fourier Transform Infrared (FTIR) spectroscopy confirmed the retention of key …
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Engineering functional biomaterials to advance environmental and resource sustainability
… sustainable Earth. First, we employed SpyTag-SpyCatcher chemistry to conjugate model proteins onto magnetic nanoparticles (MNPs), establishing a modular, magnetic-responsive platform for protein immobilization. This approach significantly enhanced protein stability while retaining …
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GENERATION OF SYNTHETIC ANTIBODIES AS POTENTIAL THERAPEUTICS TO BLOCK IL3–MEDIATED INNATE IMATINIB RESISTANCE IN CHRONIC MEYLOID LEUKEMIA
… (Fabs) to enhance targeting of CML cells. SpyCatcher-SpyTag system was used to join IL3Rα / IL3Rβ mono–Fabs by a covalent bond. The bivalent and bispecific Fab formats against IL3Rα, IL3Rβ, or IL3Rα and IL3Rβ elicited nanomolar dissociation for the targets, bound to CML cells, and impaired …
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Single-Payload Bioconjugation to Native Antibodies And A General Method for the Self-Immolative Release of Amide-Containing Molecules
… of trastuzumab. Flow cytometry and SpyTag-SpyCatcher studies carried out by Claudia Driscoll, Howarth group, showed that the ‘all-at-once’ TetraDVP concept can be used to make single-payload antibody constructs that maintain the activity and binding of the antibody and the payload. The …
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Towards ultrahigh-throughput phenotypic screening using microfluidic droplets and functionalised polyacrylamide beads
… we developed (polyacrylamide-benzylguanine-SNAP-SpyCatcher-SpyTag; Chapter 1). I find that the beads are highly permeable to proteins, and that protein-display capacity is largely determined by methacrylatebenzylguanine’s input concentration, copolymerisation efficiency and accessibility, but can …
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Development of West Nile virus candidate vaccines in Nicotiana benthamiana
… technology based on the SpyTag (ST) and SpyCatcher (SC) peptides. The immunodominant epitope of the WNV E protein, domain III (EdIII), was selected for antigen display. Two constructs of the EdIII gene were generated, one with the SC peptide on the 5'- (SC-EdIII) and the other on the 3' …
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Investigation of particulate Bluetongue virus vaccines made in plants
… through the application of the SpyTag (ST)/SpyCatcher (SC) bioconjugation method. It was anticipated that these vaccine candidates would be safe to use and allow for rapid production and scalability. Moreover, only a small fragment of the BTV8 VP2 gene would need to be modified to allow for …
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Engineering outer-membrane electron transfer proteins for modular biotic-abiotic interfacing with living cells
The electrogenic bacterium Shewanella oneidensis utilises the MtrCAB protein complex to transfer electrons across the outer membrane, forming a biotic-abiotic interface that can be harnessed for energy generation, biosensing, microbial electrosynthesis and cell signalling. Within this complex, the …