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Showing 1 to 5 of 5 for “"Split intein"”.
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Split Inteins: From Mechanistic Studies to Novel Protein Engineering Technologies
<p>Inteins are auto-processing protein domains that carry out a post-translational process known as protein splicing. This process is characterized by excision of the intein (intervening protein) domain from within a larger polypeptide sequence with concomitant ligation of the flanking extein ( …
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Protein Semi-Synthesis in Vivo
… protein is genetically tagged with one half of a split-intein. The complementary half is linked in vitro to the synthetic probe and this fusion is delivered into cells using a transduction peptide. Association of the intein halves in the cytosol triggers protein transsplicing, resulting in the …
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Conditional Protein Alleles and Regulation of Bacterial Transcription: A Thesis in Two Acts
… we term conditional protein splicing (CPS), an intein is split into two inactive fragments which are activated by heterodimerization. By fragmenting the target protein and fusing the pieces to this split intein, we were able to control the target protein's activity through splicing. In the …
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Optimizing nuclear transgene expression and genome editing for high-value compound production in the green microalga Chlamydomonas reinhardtii
… was recreated using the newly developed SpecR split intein tool for targeting two loci. Concluding all findings of this thesis, multiple novel tools were established for genetic engineering, including bicistronic expression, targeted integration, and development of a domesticated strain for …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… protein complexes. Naturally occurring split inteins can mediate spontaneous trans-splicing both in vivo and in vitro. Previous studies have demonstrated successful assembly of proteorhodopsin from two separate fragments consisting of helices A–B and helices C–G via a splicing site in …