Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"Spliceosomes"”.
-
The Role and Fate of Branch Site-U2 snRNA Interaction During Pre-mRNA Splicing
… of experimental approaches based on complete spliceosomes. We describe the in vivo analysis of the interaction between the intron branch site (BS) and its cognate region of U2 snRNA; the absolute requirement for this basepairing interaction during spliceosome assembly has previously impeded …
-
Conformation of the U12-U6atac snRNA Complex of the Minor Spliceosome and Binding by NTC-related Protein RBM22
… snRNAs for the minor variant (~0.3% of all spliceosomes in higher eukaryotes). The minor spliceosome shares key catalytic sequence elements with the major spliceosome and performs identical chemistry. Previous studies have shown that the protein-free U2-U6 snRNA complex adopts two …
-
Single Molecule Studies Of Spliceosomal Snrnas U2-U6
<p>Spliceosomes catalyze the maturation of precursor mRNAs in organisms ranging</p> <p>from yeast to humans. Their catalytic core comprises three small nuclear RNAs (U2, U5</p> <p>and U6) involved in substrate positioning and catalysis. It has been postulated, but never</p> <p>shown experimentally, …
-
Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… by the eight spliceosomal helicases. Purified spliceosomes assembled on a defined RNA substrate are analysed by UV crosslinking and next-generation sequencing, which allows for the determination of the RNA helicase binding profile at nucleotide resolution. In vitro spliceosome iCLIP …
-
Formation of an Exon-Defined A Complex Spliceosome Intermediate Results in CD45 Exon Repression
Alternative splicing is a common means for genetic regulation in higher eukaryotes, and variability in splicing patterns results in a significant increase in protein diversity. Of particular interest is how the splicing machinery is regulated to allow for alternative splicing while maintaining …
-
Splice Factor SFRS6: Regulation by p53 and Effect on IL-24 Splicing
<p>Alternative splicing of RNA transcripts is emerging as a major mechanism for expanding the proteome. Splice pattern changes are found in numerous diseases and cancers, indicating the importance of tight control over this process. While the mechanism of splicing has been described in detail, it …