Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 84 for “"Splice variants"”.
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Generating single-domain antibodies against fibronectin splice variants
… single-domain antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of tumor samples, and antibody libraries were generated. Using these libraries to pan against, I selected for …
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INTERACTIONS OF ADAM15 SPLICE VARIANTS WITH SH3 DOMAIN PROTEINS
… domain (ICD) of ADAM15 exists in multiple splice variants that contain varied combinations of polyproline regions, which interact preferentially with proteins containing Src Homology 3 (SH3) domains. Patients suffering from node-negative breast cancer exhibited a poorer prognosis when …
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Analysis of ether-à-go-go potassium channel (Eag1) splice variants in melanoma cells
… wir fanden die Expression von zwei neuen Splice-Varianten, E65 und E70, in zwei Melanom-Zelllinien (IPC298 und IGR39) und in neuralen Stammzellen. Die beiden Spleißvarianten haben keine Transmembrandomänen und bilden keine funktionale membrangebundene Kanal. Eine Charakterisierung des …
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The splice variants of TREK-1 act as dominant-negative suppressors of human uterine relaxation: Implications for preterm labor
… during gestation. In this study, three splice variants of intact human TREK-1 were identified in myometrium. For the first time, five non-conducting splice variants of TREK-1 were identified in preterm laboring and non-laboring myometrium, and were named as SV-1 to SV-5 due to the number …
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The localisation and regulation of phosphatidylinositol-4-phosphate 5-Kinase gamma splice variants and the discovery of a new mammalian splice variant, PIP5KIγ_v6
… found. PIP5KIr is subject to complex C-terminal splice variation, enhancing its transcriptional diversity through evolution and producing at least 5 known spliceoforms in the mammals. This study addresses several important questions. (1) Several remarkable differences have been discovered between …
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Isoform-Specific Expression During Embryo Development in Arabidopsis and Soybean
… resulting in the formation of more than one splice variant, a process called alternative splicing. RNA-Seq provides a major opportunity to capture the state of the transcriptome, which includes the detection of alternative spicing events. Alternative splicing is a highly regulated process …
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The Molecular Components of Estrogen Receptor Beta (ERβ) Signaling in Neuronal Sytems
… and cognitive decline. Further, alternative splice variants derived from ERβ do not bind estrogens as well as ERβ1, and importantly, ERβ splice variants increase in the brain with age. I hypothesized that altered splice variant signaling contributes to a switch in estrogen …
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Development of a Top-Down Proteomics Platform for Characterizing Myelin Basic Protein Proteoforms
… was demonstrated and was able to resolve splice variants and individual proteoforms of myelin basic protein. Data independent acquisition using nozzle skimmer dissociation yielded fragment ions that were used with novel informatics procedures to identify proteoforms of MBP. The informatics …
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The Mechanism of Action of Lidocaine and Levobupivacaine on Human Cardiac Voltage-gated Na<sup>+ </sup>Channels Na<sub>V</sub>1.5
… cancer cells express adult and neonatal NaV1.5 splice variants. Lidocaine and levobupivacaine are amide local anaesthetics that are widely used in the clinic. They have different physiochemical properties and thus differ in their pharmacokinetic and pharmacodynamic profile. We compared the …
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Top Down Mass Spectrometry of Archaea and Human Tumor Cells
… single nucleotide polymorphisms, alternative splice variants, and highly homologous gene family members.
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Splice Factor SFRS6: Regulation by p53 and Effect on IL-24 Splicing
… as a major mechanism for expanding the proteome. Splice pattern changes are found in numerous diseases and cancers, indicating the importance of tight control over this process. While the mechanism of splicing has been described in detail, it is less clear how a cell is able to modify its splicing …
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Studies Of The Molecular Mechanisms Underlying Cancer Stem Cells And The Transformed Metabolic Phenotype
… toward understanding the roles of different splice variants of the metabolic enzyme GLS in cancer cell growth. An important question in the field has concerned whether the two known GLS splice variants, glutaminase C (GAC) and kidney-type glutaminase (KGA), have redundant or opposing …
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Novel methods in transcriptome analysis using RNA-seq
… to solve two critical challenges: identifying Splice Junctions (SJs) and annotating gene fusion transcripts. Due to the limited read length of NGS data, it is extremely challenging to accurately map RNA-seq reads to SJs, which is important for the analysis of alternative splicing and isoform …
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Molecular and ontogenic analysis of the mammalian GABA<sub>A</sub> receptor
… GABA<sub>A</sub> receptor subunit mRNAs and 12 splice variants within the developing and adult murine CNS.<br></br><br></br>Preliminary studies using S 1 nuclease protection analysis demonstrated that α1, β3 and γ2 were the predominant subtypes of GABA<sub>A</sub> receptor subunits expressed at …
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Effects of salinity stress, ammonia exposure and feeding on excretory nitrogen metabolism in the marble goby, oxyeleotris marmorata
… the liver eliminates the possibility of multiple splice variants or the products of multiple genes, suggesting the covalent modification of hepatic GS in response to various experimental conditions.
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Discovering angiogenic cell signaling landscapes: Quantitative mapping through experiment and computation
… angiogenesis regulator. But several additional splice variants play prominent roles in regulating angiogenesis in health and in vascular disease, including VEGF-A121 and an anti-angiogenic variant VEGF-A165b. Despite their significance to physiological, therapeutic, and pathological …
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Calpain 9 Functions in TNF Receptor Mediated Apoptosis
… yet is essential for downstream activation. Two splice variants are reported for CAPN9. The smaller splice, CAPN9-SP2, is required for effective TNF-a induced apoptosis. CAPN9 is essential for RIPK1 recruitment and ubiquitination at the TNFR1 upon activation with TNF-alpha. CAPN9 knockdown …
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Characterisation of PACAP-responsive receptor pharmacology and expression in cell models and the spinal cord
… the PAC1 receptor may be alternatively spliced, generating variants that can differ in their agonist or signalling profiles. The PACAP peptide family show promise for the treatment of pain. To target these receptors therapeutically, we need to understand their underlying pharmacology and …
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The Regulation and Mechanism of Myelin Synthesis Involving Endogenous Steroid Hormones
… (PDGF) A-chain was detected as two distinct splice variants in Schwann cell/neuronal co-cultures. Both PDGF A-chain isoforms were characterized and examined throughout the myelination process. The mRNA for the long form PDGF A-chain was found to be induced during myelin synthesis and was …
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
… of a simple and rapid method to detect RNA splice variants using biarsenical dyes and split tetracysteine moieties, which may accelerate biochemical studies of alternative splicing and identification of factors that modulate RNA splicing.
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