Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"Splice Variant"”.
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Role Of d16HER2 Splice Variant In Breast Cancer Stem Cells
… the potent oncogenicity driven by d16HER2 variant vs WTHER2 unveiling its direct functional interplay with the activated tyrosine kinase SRC that was found to be coupled with d16HER2 expression/activity in HER2+ BCs. Further, pre-clinical and clinical data indicated that d16HER2 activation …
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Molecular and Pharmacological Characterization of the Rat RAMP2B Splice Variant
… CL complex. A partial sequence of a rat RAMP2 splice variant was previously identified in our laboratory. I hypothesized that human tissue expresses RAMP2 splice variants and that a clone of the full-length rat RAMP2 splice variant forms an adrenomedullin receptor with decreased function in the …
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Identifying the signalling pathway of a novel Myostatin Splice Variant (MSV)
… The discovery in our laboratory of a novel splice variant of Mstn (MSV) that opposes Mstn and stimulates the proliferation of myoblasts provided the impetus for the investigations in this thesis. The splicing of MSV was restricted to the Cetartiodactyl clade of mammals, and MSV may represent …
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The Role Of Pdgf C And Its Splice Variant In Breast Cancer
… During the course of our study, we discovered a splice variant of PDGF C encoding the truncated PDGF C protein (t-PDGF C). Specific aims of this dissertation are to determine the role of full-length PDGF C (FL-PDGF C) and t-PDGF C in breast cancer and to characterize their subcellular …
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The localisation and regulation of phosphatidylinositol-4-phosphate 5-Kinase gamma splice variants and the discovery of a new mammalian splice variant, PIP5KIγ_v6
… found. PIP5KIr is subject to complex C-terminal splice variation, enhancing its transcriptional diversity through evolution and producing at least 5 known spliceoforms in the mammals. This study addresses several important questions. (1) Several remarkable differences have been discovered between …
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The splice variants of TREK-1 act as dominant-negative suppressors of human uterine relaxation: Implications for preterm labor
… during gestation. In this study, three splice variants of intact human TREK-1 were identified in myometrium. For the first time, five non-conducting splice variants of TREK-1 were identified in preterm laboring and non-laboring myometrium, and were named as SV-1 to SV-5 due to the number …
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The Regulation and Mechanism of Myelin Synthesis Involving Endogenous Steroid Hormones
… (PDGF) A-chain was detected as two distinct splice variants in Schwann cell/neuronal co-cultures. Both PDGF A-chain isoforms were characterized and examined throughout the myelination process. The mRNA for the long form PDGF A-chain was found to be induced during myelin synthesis and was …
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Towards understanding EC coupling protein expression with age
… EC coupling. Furthermore, RyR1 has two variably spliced regions, ASI and ASII, thought to be developmentally regulated. The ASI region, which lies close to a DHPR beta1a binding site, affects EC coupling. The juvenile isoform (ASI(-)RyR1) shows enhanced calcium release during EC coupling and its …
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Towards understanding EC coupling protein expression with age
… EC coupling. Furthermore, RyR1 has two variably spliced regions, ASI and ASII, thought to be developmentally regulated. The ASI region, which lies close to a DHPR beta1a binding site, affects EC coupling. The juvenile isoform (ASI(-)RyR1) shows enhanced calcium release during EC coupling and its …
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Visualizing the dynamics of ionotropic glutamate receptors using atomic force microscopy
… the ATDs of the (rapidly desensitizing) flop splice variant of the AMPA receptor were more mobile than those of the (more slowly desensitizing) flip splice variant. I suggest that the greater mobility of the flop splice variant might be connected with its more short-lived functional response …
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Blimp-1 Regulates Terminal Differentiation of T Cells
… primary T lymphocytes, particularly its minor splice variant. Ectopic expression of Blimp-1 by retroviral transduction neither dramatically altered secretion of IFN-ã or IL-4 nor did it induce the ability to suppress as regulatory T cells. However, induction of Blimp-1 resulted in not only a …
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Development of a Top-Down Proteomics Platform for Characterizing Myelin Basic Protein Proteoforms
… was demonstrated and was able to resolve splice variants and individual proteoforms of myelin basic protein. Data independent acquisition using nozzle skimmer dissociation yielded fragment ions that were used with novel informatics procedures to identify proteoforms of MBP. The informatics …
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MECHANISMS OF SUBSTRATE DEGRADATION AND RECRUITMENT BY CULLIN E3 LIGASES
… associates with Skp1. Lastly, Skp1a, a splice variant of the commonly-studied Skp1b protein, is characterized for the first time. Our results suggest that Skp1a may have alternate functions from Skp1b.
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The C-terminal domains of leucyl-tRNA synthetases
… of the canonical C-terminal domain. An annotated splice-variant of the mitochondrial LeuRS hints at a mechanism for subcellular localization. Deletion of CECL from the chromosome prevents homozygous worms from surviving fertilization in most cases. From these observations, we hypothesize that the …
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Factors Promoting Human Papillomavirus Mediated Cervical Carcinogenesis
… factors, such as expression of E6*, a splice variant of the E6 oncogene, or by exogenous factors led to DNA damage in normal oral keratinocytes and in cervical keratinocytes containing episomal HPV16. We found that OS increased the integration rate for both foreign DNA and HPV, while …
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Mammalian TIMELESS and Protein Kinase G: Partners Linking the Molecular Clockwork to Environmental Light
… length protein (mTIM-fl) and the other a short splice variant (mTIM-s) corresponding to the last third of mTIM-fl. Only mTIM-fl oscillates in the SCN, associates with other core clock elements, is necessary for circadian rhythmicity, and is degraded by light and GLU in the late night. These …
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Regulation of lipid signaling at the Golgi by the lipid phosphatases hSAC1 and OCRL1
… inhibited in OCRL1 depleted cells. The OCRL1-b splice variant populates TGN and early endosomal compartment whereas the OCRL1-a splice variant containing an extra 8 amino acid acidic cluster is found only in a subset of late endosomal/ lysosomal membranes. This distinct localization of OCRL1 …
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MODULATION OF VOLTAGE-GATED CALCIUM CHANNEL BY A SYNAPTIC PROTEIN AND GANGLIOSIDE
… calcium channels in an isoform- and splice-variant-selective manner. Notably, the CaV2.1 and CaV2.2 channels are mutually exclusively spliced to produce either CaV2.1[EFa] or CaV2.1[EFb], and CaV2.2[EFa] or CaV2.2[EFb] respectively. Snapin selectively increased the current density of …
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Von Hippel-Lindau Syndrome: Characterization of a Potentially Novel VEGF-A Isoform and Elucidation of Molecular and Vascular Mechanisms of Observed Phenotypic Changes
… experiments revealed a potentially novel VEGF-A splice variant with excision of the VEGF Receptor-1 (VEGFR-1)/Flt-1 binding domain, rendering this isoform resistant to native down-regulation. Additionally, phenotypic changes were observed in adult VHL mutant mice, specifically very red appearing …
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A Role for glutamate and Gaba in attention-deficit/hyperactivity disorder: a study of the spontaneously hypertensive rat
… expression of the glutamate transporter GLT1b splice variant and KCC2 was increased in SHR hippocampus compared to WKY, while GLT1 total was reduced in SHR hippocampus compared to WKY and SD. Experiencing early life stress did not influence behaviour of SHR, and had limited effect on the …
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