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Showing 1 to 20 of 40 for “"Spindle Assembly Checkpoint"”.
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… cells employ a surveillance mechanism termed Spindle assembly checkpoint that senses the lack of tension/attachment on the kinetochores and consequently blocks anaphase onset by inhibiting an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… cells employ a surveillance mechanism termed Spindle assembly checkpoint that senses the lack of tension/attachment on the kinetochores and consequently blocks anaphase onset by inhibiting an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, …
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Genetic and biochemical analysis of the mammalian spindle assembly checkpoint
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 2001.
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Examining the Regulation of Cell Division by the Spindle Assembly Checkpoint
… The molecular surveillance mechanism called the spindle assembly checkpoint (SAC) inhibits chromosome segregation before all chromosomes are correctly attached (bi-oriented) on the microtubule spindle to prevent segregation errors that may lead to chromosomal instability and cell death. In this …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… with its essential co-activator Cdc20. The spindle assembly checkpoint (SAC) is a surveillance mechanism that monitors the chromosomes' interactions with the microtubules of the mitotic spindle apparatus. In response to even one erroneous attachment the affected kinetochore emits a "wait …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
The spindle assembly checkpoint and the DNA damage response have been studied for decades as independent pathways exerting separate functions. However, crosstalk between these cellular processes has emerged. The extent of overlap between these pathways is still unknown, especially in multicellular …
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Histone H3 K4 Methylation Regulates The Spindle Assembly Checkpoint Through Direct Binding of Multiple Checkpoint Components and Cdc20
… unknown role in regulating mitosis through the Spindle Assembly Checkpoint. The Spindle Assembly Checkpoint ensures duplicated chromosomes are segregated correctly and each daughter cell receives one full copy of the genome. Our data show <em>SET1 </em>mutants and histone H3K4 mutants display a …
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Investigation of the role of chromosome missegregation in embryo development and in tumorigenesis
… receive the correct number of chromosomes. The spindle assembly checkpoint prevents chromosome missegregation by arresting the cell before sister chromatid separation if any one chromatid is not properly attached to the spindle microtubules. Defects in chromosome segregation lead to the creation …
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Investigating mitotic vulnerabilities that arise upon oncogenic cell transformation
… to microtubules (MTs) emanating from the spindle poles. Failure to undergo high-fidelity cell division can result in aneuploidy and even progress to continued mis-segregation in a phenomenon known as chromosome instability (CIN). As aneuploidy results from defective pathways in mitosis, it …
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TO INVESTIGATE THE ROLE OF RNF8 IN REGULATING MITOTIC PROGRESSION IN GLIOMAS
… damage response factor, has been implicated in spindle assembly checkpoint (SAC) but its exact role remains unknown. Using RNF8 proximity proteomics, we find that RNF8 interacts with MAD2 and CAMK2D via its RING and FHA domains respectively to activate the SAC response. The physiological …
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Role of Ame1, a central component of the Saccharomyces cerevisiae kinetochore, in kinetochore function and structure
… and the supervision of these events by the spindle assembly checkpoint were analyzed in the ame1-2 mutant: • The Ame1 protein is needed for the establishment of bipolar attachments to microtubules emanating from opposing poles. Under tension one of the attachments is lost due to a structural …
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Functional analyses of microtubule and centrosome-associated proteins in Dictyostelium discoideum
… TACC-binding XMAP215 protein to centrosomes or spindle poles. By contrast, fluorescence recovery after photobleaching (FRAP) analyses conducted in this study demonstrate that in Dictyostelium recruitment of CP224 to centrosomes or spindle poles is not perturbed in the absence of TACC. Instead, …
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PP2A-B56 isoform specificity at the centromere and kinetochore
… by the PP2A-B56 phosphatase complex: the Spindle Assembly Checkpoint (SAC), kinetochore-microtubule attachments and sister chromatid cohesion. We show here that these key functions of PP2A-B56, which require its localization to either the kinetochore or centromere, are split between …
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Investigating the Relationship Between Calorie Restriction and Mitotic Protein BubR1 in the Prevention of CIN-Induced Tumorigenesis
… diseases through its mitotic functions in the spindle assembly checkpoint response and stabilization of chromosome-microtubule interactions. BubR1 protein levels decline naturally with age, which may lead to increased incidence of aneuploidy, and therefore cancer risk. Calorie restriction (CR) …
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Exploring the effects and efficacy of novel cell cycle regulating compounds in aggressive Breast, Ovarian and Cervical Cancer
The Spindle Assembly Checkpoint (SAC) is a cell signalling system that regulates cell division. SAC ensures the correct separation of chromosomes during mitosis. Defects in SAC can result in genome instability, increasing the risk of cancer development and making it a potential therapeutic target …
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Mitotic slippage by ATP depletion and its mechanism
… known that tumor cells possess two main energy checkpoints monitoring the amount of available ATP during G1 phase and G2 to M transition. However, it is still uncertain the existence of energy checkpoint during mitotic progression. In our hand, ATP depletion by the co-treatment with 2-deoxy …
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Constitutively active-RSK2 induces chromosomal instability through diverse mechanisms
… RSK2 induced chromosomal instability through spindle assembly checkpoint defects and kinetochore-microtubule (KT-MT) attachment defects. Since RSK2 localizes in microtubule during mitosis, we focused on the relationship between RSK2 and microtubule. We nalyzed kinetochore-microtubule (KT-MT) …
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The role of ZW10 and its binding partners in chromosome segregation
… chromosomes to microtubules of the mitotic spindle in a bipolar fashion and monitor the state of the kinetochore-microtubule attachment. In case errors are present, a monitoring mechanism, the spindle assembly checkpoint, inhibits metaphase to anaphase transition until proper bipolar …
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Changes in Kinetochore Structure and Molecular Composition in Response to Mis-attachment
… to microtubules (MTs) from opposite mitotic spindle poles. KT mis-attachments can occur in early mitosis and cause chromosome mis-segregation and aneuploidy if not corrected. These mis-attachments include monotelic (one attached and one unattached sister KT), syntelic (both sister KTs …
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