Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Sphingomyelinase"”.
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Spatial and temporal control of lipid-membrane morphology induced by sphingomyelinase
Sphingomyelinase (SMase) has been shown to be involved in a variety of cell regulation processes. It can convert sphingomyelin (SM) to ceramide (Cer) and has been suggested to influence the cellular processes by reorganizing the cell membrane morphology. This thesis aims at a more comprehensive …
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Role of <i>Ixodes scapularis</i> Sphingomyelinase-Like Protein (IsSMase) in Tick Pathogen Interactions
… virus (TBEV) and Powassan virus (POWV). Sphingomyelinase-like protein (IsSMase, a Sphingomyelinase D or SMase D, a venomous protein ortholog of spiders) is an enzyme present in ticks that helps to catalyze the hydrolysis of the sphingomyelin (cell membrane lipid) into phosphocholine and …
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ROLE OF ACID SPHINGOMYELINASE IN ICAM-1/NHE1-DEPENDENT ENDOCYTOSIS: IMPLICATIONS IN LEUKOCYTE TRANSMIGRATION
… CAM-mediated pathway associates with acid sphingomyelinase and ceramide. This supports plasmalemma deformability and cytoskeleton rearrangement, bridging these events to the formation of endothelial docking structures and vesicles involved in leukocyte transmigration.
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The role of ceramides in cigarette smoke-induced alveolar cell death
… Exposure to CS activated within minutes the acid sphingomyelinase, and within weeks the de novo- ceramide synthesis pathways. Pharmacological inhibition of acid sphingomyelinase significantly attenuated CS-induced apoptosis. To understand the mechanisms by which ceramides induce apoptosis, we …
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Investigations into the digestive system proteases of Argiope aurantia (Araneae: Araneidae), hemolytic agents of spider venoms, and the role of both in necrotic arachnidism
… fluid proteases and spider venom enzymes (e.g., sphingomyelinase D and phospholipase A2) for their potential to cause necrotic lesions, as both are thought to be primary agents.</p><p>The digestive fluid of Argiope aurantia was examined for its ability to cleave a variety of extra-cellular matrix …
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Sphingomyelinases and Lysosomal Trafficking in Ebolavirus Entry
… During lysosomal exocytosis, pH-dependent acid sphingomyelinase (ASM) is released to the plasma membrane‟s outer leaflet, resulting in ASM-dependent macropinosome formation. Because EBOV enters via a macropinocytosis-like mechanism, the role of ASM in infection was assessed. Specific ASM …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… study demonstrated that expression of neutral sphingomyelinase (NSMase), an enzyme that hydrolyzes sphingomyelins to generate ceramide, is regulated by Group VIA phospholipase A2 (iPLA2β)-dependent mechanism during β-cell apoptosis. This prompted us to hypothesize that iPLA2 is upstream of …
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Factors affecting transduction efficiency of pseudotyped viral vectors incorporating alphaviral glycoproteins
… pseudotypes transduce the acid sphingomyelinase deficient (ASMase-/-) cells derived from Niemann Pick’s disease-model mice more efficiently than the cells from healthy mice (ASMase+/+). Thus alphavirus-glycoprotein pseudotypes hold a potential as suitable vectors for gene …
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Attenuation of Corynebacterium pseudotuberculosis by targeted mutagenesis of the phospholipase D gene.
… isoelectric focusing. In the purest fraction, sphingomyelinase, synergistic hemolysis, and staphylococcal β-hemolysin inhibition activities were associated with the presence of 31 and 21 kDa proteins. Only the 31 kDa protein was identified by Western blot analysis with PLD-neutralizing …
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Understanding the mechanisms of phosphatidylserine exposure in sickle cells
… on protein kinase C (PKC). Furthermore, the sphingomyelinase (SMase) signalling pathway was also explored to identify its role in PS exposure. Results with a SMase inhibitor and ceramide on RBCs of SCD patients showed a strong correlation between SMase activity and PS exposure. Moreover, …
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Analysis of rat brain lipids and metabolites after antidepressant drug treatment
… of the brain sphingolipid pathway namely, acid sphingomyelinase (ASM) and acid ceramidase (AC). By using real-time quantitative polymerase chain reaction (RT-qPCR) it was shown that paroxetine and desipramine significantly reduced mRNA levels of ASM in the HP while effects in the PFC and ST did …
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Investigating sphingolipid behavior and function using metabolic labeling
… membrane reorganization, the involvement of acid sphingomyelinase or sphingomyelin phosphodiesterase 1 (SMPD1), a sphingolipid metabolizing enzyme, in influenza virus infection and particularly its entry was evaluated using RNAi and a pharmacological inhibitor. Western blotting performed prior to …
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Exploring the roles of key lipids in the influenza virus lifecycle
The influenza virus is a common respiratory pathogen with the capacity to cause global pandemics affecting millions of people, contributing substantially to overall mortality and morbidity. Thus, considerable research focuses on the prevention and treatment of influenza virus infection. Despite the …