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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"Sphingomyelin"”.
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Role of the sphingomyelin/ceramide pathway in diabetic retinopathy
Thesis (Ph. D.)--Michigan State University. Department of Microbiology and Molecular Genetics, 2010
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Sphingomyelin as a danger signal in cell-autonomous immunity
… receptor, Galectin 8. My research reveals that sphingomyelin, a host lipid predominantly located on the outer leaflet of the plasma membrane, is exposed to the cytosol on damaged BCVs. I visualised the appearance of intracellular sphingomyelin by utilising Lysenin - a sphingomyelin-specific …
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Molecular Dynamics of p21 and Fluorescent Sphingomyelin in Keratinocytes Exposed to UVB
… p21 within keratinocytes that were incubated in sphingomyelin (SM) and later exposed to UV. Confocal microscopy and automated image processing provided the tools to assess large populations of keratinocytes in the effort to accurately identify the photoprotective qualities of sphingomyelin. …
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The Use of Sphingomyelin to Protect Against UV Induced DNA Damage in Human Keratinocytes
… effects of sunscreen. In particular, bovine milk sphingomyelin (SM) is a compound that has the potential to protect against UV damage without any of the dangerous side effects of sunscreen. Here we present the use of SM for UV protection of human keratinocytes (KRTs) to prevent DNA mutations that …
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Investigation of Experimental Variation of Bovine Sphingomyelin as a Novel Ingredient for Ultraviolet Protection
… an alternative solution that uses bovine sphingomyelin (BSM) as photoprotective solution against UV irradiation.</p> <p>In order to evaluate the effectiveness of BSM against UV radiation, p21 intensity was measured on a monolayer of keratinocytes, as the intensity directly correlates to …
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Molecular characterization of a sphingomyelin binding site in the vacuolating cytotoxin of H. pylori
… been determined that the membrane phospholipid sphingomyelin (SM) is the primary receptor for the Helicobacter pylori vacuolating cytotoxin (VacA). While the cellular receptor has been identified, the molecular basis of SM recognition remains unknown. Molecular modeling of unrelated SM-binding …
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Assessing the Photoprotective Effects of Fluorescent Sphingomyelin Against UVB Induced DNA Damage in Human Keratinocytes
… particular, 24 hour incubation with bovine milk sphingomyelin (BSM) has demonstrated photoprotective potential by reducing p21 and p53 levels in keratinocytes (KRTs) after UV radiation [13], [14]. This thesis aims to expand on past BSM research by exploring the mechanism for photoprotection. …
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Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
The Golgi complex is the central station along the secretory pathway to sort proteins and lipids to their final destinations. It exists of a series of flattened cisternal membranes that are aligned in parallel to form polarized stacks. This structure is actively maintained amid a large flow of …
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An Oxanthroquinone Derivative Disrupts Ras Plasma Membrane Localization and Function By Inhibition of Acylpeptide Hydrolase and Perturbation of Sphingomyelin Metabolism
… we observed that G01 also enhanced cellular sphingomyelin (SM) and ceramide levels and disrupted the localization of several lipid and cholesterol reporters, suggesting that the G01 molecular target may involve SM metabolism. Indeed, G01 exhibited potent synergy with other compounds that …
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Identification and Characterization of the Helicobacter Pylori Vacuolating Cytotoxin Receptor
… and abundant plasma membrane sphingolipid, sphingomyelin, as a cellular receptor. Cellular sphingomyelin levels correlated strongly to sensitivity to VacA. Modulation of sphingomyelin levels specifically at the plasma membrane altered VacA-mediated intoxication. However, cells with prebound …
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Suppression of intestinal inflammation and inflammation-driven colon cancer in mice by dietary sphingomyelin: Importance of peroxisome proliferator-activated receptor γ expression
… sodium sulfate (DSS)-induced colitis by dietary sphingomyelin in mice that lack functional peroxisome proliferator-activated receptor γ (PPAR-γ) in intestinal epithelial and immune cells. Dietary spingomyelin decreased colonic inflammation in mice of both genotypes but more efficiently in mice …
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Interactions of disruptive materials with phospholipid bilayers
… A correlation between the presence of sphingomyelin or cholesterol and increased pore formation is identified by the increase of potassium leakage through vesicles and formation of raft structures visible with AFM. ATR-FTIR shows that alamethicin disrupts all types of bilayers studied, …
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Adjuvant Interactions with Lipid Membranes and Their Effect on Cellular Immune Responses
… adjuvants (ABAs) have high affinity for sphingomyelin and cholesterol, which allowed them to activate dendritic cells exclusively through lipid sorting. This dissertation sought to understand how lipid interactions contribute to the immunostimulatory properties of adjuvants. The membrane …
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Lipid studies in breath-hold diving mammals and obese, pre-diabetic mice.
… significantly higher C16 ceramide, 16:0 sphingomyelin, and dipalmitoylphosphatidylcholine (DPPC) levels compared to deeper divers (Weddell seals, Leptonychotes weddellii). Additionally, in comparing ringed seal (Pusa hispida) skeletal muscle to liver tissue, liver tissue showed …
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A Study of the Membrane Binding Properties of the Matrix Protein from Human Respiratory Syncytial Virus
… interacts differently with monolayers containing sphingomyelin, which in combination with cholesterol forms phase separated liquid-ordered (Lo) microdomains. RSV M was found to bind extrinsically to the sphingomyelin-containing Lo domains. This did not occur when the protein was in contact with …
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Studies on Bioactive Lipid Mediators Involved in Brain Function and Neurodegenerative Disorders. The effect of ¿-3PUFA supplementation and lithium treatment on rat brain sphingomyelin species and endocannabinoids formation; changes in oxysterol profiles in blood of ALS patients and animal models of ALS.
… dietary compliance and to investigate brain sphingomyelin species of adult and aged rats supplemented with n-3 EPA and n-3 DPA to evaluate the effects and benefits on age-related changes in the brain. Furthermore, to study the effects of lithium on the brain FA-EAs and ECs to further …
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Formation of membrane domains induced by the G and M proteins of vesicular stomatitis virus
… incorporated into vesicles at the same time, sphingomyelin and cholesterol were also sequestered into domains. Both the G and M proteins co-localized with the domains induced by the proteins. Thus, the G and M proteins together could induce the formation of large domains enriched in PA, PS, …
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Mechanisms of Multivesicular Body Biogenesis and Exosome Release
… reduzierte die Hemmung von neutralen Sphingomyelinasen die Freisetzung von Exosomen. Daher wurden GUVs (giant unilamelar vesicles) mit Sphingomyelin hergestellt, in denen zwei unterschiedlichen Phasen vorlagen. Die Hydrolyse von Sphingomyelin an der äußeren Schicht der GUV bewirkte …
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Protein Sensors for Membrane Cholesterol
… membranes only when they contain cholesterol and sphingomyelin. I developed fluorescently labeled ostreolysin A as a tool to probe for sphingomyelin-cholesterol complexes in the plasma membranes of mammalian cells. In ongoing work, I am screening for compounds that bind anthrolysin O, as …
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Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments
… activity that is mediated by the increased sphingomyelin and glycosphingolipid content in potassium-deficient BBM, although the mechanisms by which these lipids may modulate NaPi-IIa activity have yet to be determined.
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