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Showing 1 to 20 of 40 for “"Spermidine"”.
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Regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli by putrescine, spermidine and cAMP
… putrescine. The two polyamines, putrescine and spermidine, and the cyclic AMP (CAMP) - cAMP receptor protein (CRP) are known to inhibit the expression of ODC via undefined mechanisms. A single copy of the speC’-lacZ fusion plasmid pOL-1, containing an 843 base pair fragment including the spec …
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Characterization of the expression profile of polyamine biosynthetic genes (Spermidine synthase) and polyamine metabolic regulation in arabidopsis
… genes encoding the polyamine biosynthetic enzyme spermidine synthase ( AtSPDS1 and AtSPDS2) were studied in Arabidopsis at different developmental stages using promoter::reporter approach. The two homologues showed similar ubiquitous expression with subtle differences being observed in certain …
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A study of the Project for American Studies in the Secondary Schools and its effect on curricular change
… the effect of the exogenous polyamines, spermidine and spermidine, on growth of crown gall tumors, to assay levels of spermidine and spermine in normal and crown gall tumor tissue and to determine the effects of selected anti-tumor compounds on growth of crown gall tumors as well as on …
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The Effect of Spermidine on the Rate of Sex-Linked Recessive Lethals Caused by X-Ray in Drosophila Melanogaster
The problem. Spermidine is a polyamine naturally occurring in many organisms including "Drosophila melanogaster". It has been shown to decrease the heat denaturation of DNA by binding to the DNA double helix. The agent has also decreased the development of resistance of bacteria to a particular …
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The Role of S-Adenosylmethionine Decarboxylase on Regulation of Polyamine and Trypanothione Metabolism in Trypanosoma Brucei
… a key enzyme required for the synthesis of spermidine and trypanothione, and examined the role of AdoMetDC on the regulation of polyamine and trypanothione metabolism. The recombinant T. brucei AdoMetDC enzyme displays low catalytic efficiency as compared to the human enzyme (1000 fold …
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Toxicology and pharmacology of N¹-acetylspermidine and N⁸-acetylspermidine
… tissues. N<sup>1</sup>- and N<sup>8</sup>- acetylspermidine have been known for a considerable time as normal constituents in human urine, but their physiological role is unknown. Recent studies have indicated the presence of enzymes in the tissues capable of converting spermidine into …
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Essentiality and Regulation of Deoxyhypusination in Trypanosoma brucei
… T. brucei and other trypanosomatids require spermidine for the formation of trypanothione, a unique thiol-redox factor. In other eukaryotes, spermidine is essential for the (deoxy)hypusination of eukaryotic initiation factor 5A (eIF5A). Hypusination, a post-translational modification, occurs …
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THE REGULATION OF POLYAMINES AND THEIR ROLE IN THE PROLIFERATION OF HELA CELLS
… by the addition of 0.5 (mu)M-spermine or spermidine or 10 (mu)M-putrescine to the HeLa cell cultures at the time of refeeding with fresh medium. Much higher concentrations (1 mM) of the non-physiological diamines, 1,3-diaminopropane or 1,3-diamino-2-hydroxy-propane, were required to …
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The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication
… plants and animals. The polyamines spermine, spermidine and putrescine have been of primary interest, as they participate in cell growth and proliferation and thus are involved in health, disease and aging. Polyamines are also reported as secondary metabolites in stress response in plants. …
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The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis
… and in the concentrations of putrescine, spermidine, and spermine. DFMO application inhibited Odc activity and reduced polyamine levels after hypoosmotic exposure (0.1 ppt). Although gill putrescine levels remained elevated throughout the first 7 d post transfer to 0.1 ppt, the …
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ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ C3HB
… APPEARING LATE. OF THE POLYAMINES TESTED SPERMIDINE WEAKLY ACTIVATES AT LOW CONCENTRATIONS AND SPERMINE IS HIGHLY INHIBITORY EVEN AT LOW CONCENTRATIONS. INCREASING CONCENTRATIONS OF SPERMIDINE INTENSIFY LABELLING OF AT LEAST TWO PROTEINS WITH M.W 14.5 AND 15.5 KD. EXOGENOUS MRNA (GLOBIN …
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DEVELOPMENT OF A LC/MS/MS ENZYME METHOD FOR N8 - ACETYLSPERMIDINE MEASUREMENTS IN ENZYME ASSAYS
… of a way to study the N<sup>8</sup> - acetylspermidine deacetylase enzyme activity. The method created in this thesis emphasizes sensitivity, accuracy and safety.</p> <p>In this study, HeLa cells were cultured and extracted to yield a crude N<sup>8</sup> - acetylspermidine deacetylase enzyme …
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Transgenic manipulation of polyamine metabolism in poplar
… amounts of putrescine, a small increase in spermidine, and a small reduction in spermine as compared with non-transgenic (NT) cells. The conversion of labeled ornithine into putrescine was significantly higher in the transgenic than the NT cells.</p><p>The results show that: (a) Transgenic …
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Investigations into the molecular evolution of plant terpene, alkaloid, and urushiol biosynthetic enzymes
… N-methyltransferase) or primary metabolism (spermidine synthase) by attempting to interconvert a spermidine synthase to encode putrescine N-methyltransferase activity based upon past patterns of natural selection. In contrast to the HPS/TEAS system, using delta dN/dS metrics between SPDS and …
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EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT
… for the synthesis of the essential polyamines spermidine and spermine. In the target tissue of "Pneumocystis", the lung, levels of AdoMet were previously shown to be depleted experimentally using nicotine. Here we show that chronic administration of nicotine in an animal model of PcP resulted …
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Development and characterization of cell-free amino acid incorporation systems from <u>Cellvibrio gilvus</u>
… Polyamiaes (putrescine, spermine, and spermidine) had a slight stimulatory effect on poly U-directed.¹⁴C-phenylalanine incorporation, but no effect on ¹⁴C-Leucine incorporation.
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Polyamine metabolism in Arabidopsis: Transgenic manipulation and gene expression
<p>The metabolism of polyamines (Putrescine, Spermidine and Spermine) in wild type and transgenic Arabidopsis thaliana (Col 0 ecotype) plants was studied using the techniques of transgenic manipulation and gene expression. Two specific objectives were: (1) To study the effects of inducible and …
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Understanding polyamine metabolism through transgenic manipulation in poplar suspension cultures
… occurring polyamines in plants are putrescine, spermidine, and spermine. Suspension cultures of poplar (Populus nigra x maximowiczii), transformed with a mouse ornithine decarboxylase gene (under the control of a 2X 35S CaMV promoter) were used to study the impact of up-regulation of putrescine …
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