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Showing 1 to 20 of 175 for “"Spermatozoa"”.
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Cryopreservation of microencapsulated bovine spermatozoa
The ultimate design of a microencapsulated AI dose is to continuously release sperm over a period of time in the female reproductive tract, thus alleviating the need for estrus detection. The objective of Trial 1 was to determine in vitro sperm release times for three microcapsule membranes. Semen …
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New methods for cryopreserving rooster spermatozoa
Rooster spermatozoa sustain permanent membrane damage during cooling, thawing and dilution due to inappropriate changes during the phase transition and osmotic intolerance. Glycerol is a common cryoprotectant used to protect cells during freezing, but its relatively large molecular weight and …
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Apoptotic markers in ejaculated human spermatozoa
… of apoptosis with morphological features in spermatozoa in the same samples. The three sample groups were identified as: teratozoospermic [G-pattern] (n=26), teratozoospermic [P-pattern] (n=98) and oligoteratozoospermic [Ppattern] (n=36). Standard semen analysis was conducted on the semen …
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Cryopreservation of Domestic Cat Epididymal Spermatozoa
… still effectively freeze domestic cat epididymal spermatozoa, for the purpose to sort spermatozoa into X and Y, using flow cytometry. In the first experiment we compared a TesT (Tes + Tris) 20% egg yolk extender (control) to a modified human sperm preservation medium (HSPM) and a Tris citrate …
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The development of motility in spermatozoa
Mammalian spermatozoa acquire the capacity for motility during passage through the epididymis. This study on rat spermatozoa shows that pH, cAMP and protein kinase C (PKC) all play an important role in the initiation of motility. pH has the most critical role and until the initial pH change in …
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Localization of FC Receptors on Murine Spermatozoa.
… serum binds to the acrosomal region of spermatozoa of a number of species (Weil and Finkler, 1959; Edwards, 1960). Beck et al. (1962) demonstrated by immunofluorescent techniques that this serum component is an immunoglobulin molecule and it can bind to both the acrosome and tail region …
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The response of human spermatozoa to chemoattractants
… to bourgeonal was inhibited. Pretreating spermatozoa with bis-phenol (20μM) to release stored Ca\(^{2+}\) did not alter the response. Thus bourgeonal acts primarily by inducing Ca\(^{2+}\) influx. Treatment of sperm with bourgeonal caused an increase in [cAMP]. When cells were pretreted …
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Studies on the Metabolism of Bovine Spermatozoa
Made available in DSpace on 2014-12-05T23:08:01Z (GMT). No. of bitstreams: 1 6206143.pdf: 5896593 bytes, checksum: 0229ce1a41637e7b1a3b21c1f77e0a6c (MD5) Previous issue date: 1962
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Long-term storage of liquid boar spermatozoa
… (b) evaluate the fertilizing capacity of stored spermatozoa using the optimum extender system; (c) critically analyze enzymatic and morphological changes associated with storage and aging of boar spermatozoa; and (d) characterize properties of boar spermatozoa important to fertilization. The …
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CatSper: Characterisation in Human Spermatozoa and Clinical Significance
… for initiation of hyperactive motility in murine spermatozoa, and was previously identified to be disrupted in sperm from an infertile patient. However, little is known about the incidence of CatSper defects in the IVF (in-vitro fertilisation) patient population.<br/><br/>In the present study two …
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Compounds Modulating Calcium and Motility in Human Spermatozoa
… that induced an increase in [Ca2+]i in human spermatozoa in-vitro. This thesis investigated the effect of this [Ca2+]i using techniques including CASA, Kremer tests and Flow cytometry to study compound effects on human spermatozoa motility and function. Some drugs demonstrated positive effects …
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Carbon Dioxide Uptake and Fixation by Bovine Spermatozoa
Made available in DSpace on 2014-12-12T00:36:22Z (GMT). No. of bitstreams: 1 7114835.pdf: 4911426 bytes, checksum: 42a27b14b3b41857cc7648c09dd04b25 (MD5) Previous issue date: 1970
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Apoptosis Pathways: Presence and significance in Ejaculated Human Spermatozoa
<p>Ejaculated sperm display markers that are indicative of apoptosis in somatic cells. The question remains as to whether sperm have operative apoptosis mechanisms. The aim of this research was to test the hypothesis that apoptosis markers in sperm and somatic cells are different.</p> <p>Ejaculated …
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Chromosomes and spermatozoa of the great apes and man
… related species. Comparative studies of the spermatozoa of the great apes and man were undertaken and showed that man was not unique in producing pleiomorphic spermatozoa, since this feature was also present in the gorilla. Moreover, the morphology of human and gorilla spermatozoa resembled …
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Factors affecting preservation of liquid and frozen ram spermatozoa
… 2 vividly demonstrates the vulnerability of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress, there was more variation among treatments reflected in maintenance of both intact acrosomes and motile life (Experiment 2, Table 9) than when …
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Factors Influencing the Transport of Spermatozoa in Artificially Inseminated Gilts
Made available in DSpace on 2014-12-09T14:28:48Z (GMT). No. of bitstreams: 1 6507071.pdf: 2480642 bytes, checksum: eadeb31e27d61d6bb42565b3d6d98de6 (MD5) Previous issue date: 1965
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Regulation of Volume by Spermatozoa and Its Significance for Conservation Biology
… of the volume regulatory mechanism of spermatozoa could produce options to improve the cryopreservation of spermatozoa and provide a non-hormonal contraceptive option for men, both of which could have significant impacts on global biodiversity preservation. Volume regulation of somatic …
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Choline Acetyltransferase and Carnitine Acetyltransferase Activity in Human Spermatozoa During Capacitation
… regional distribution of ChAT activity in human spermatozoa is altered during in vitro capacitation and it correlates with the fertilizing potential of sperm.</p> <p>Regional immunoreactivity in human spermatozoa as assessed by fluorescent immunocytochemistry was compared with ChAT and CaAT …
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