Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Spermatids"”.
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3D localization of cytoskeleton in mouse spermatids using stochastic optical reconstruction microscopy
… the manchette, protein and vesicle transport in spermatids, and sperm head shaping are crucial steps in the formation of healthy sperms. The cytoskeleton in spermatids plays a crucial role in shaping the sperm head. The acroplaxome exerts forces on the nucleus and gives the mammalian sperm head …
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Analysis of the role of the X/Y homologous gene family Xmr/Sly in murine spermiogenesis
… that is expressed in round and early elongating spermatids. SLY interacts with the acrosomal protein DKKL1, suggesting that SLY may be involved in the development or function of the acrosome, a structure that contains digestive enzymes required for fertilisation. Secondly, the role of Xmr in …
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Programming of the paternal nucleus for embryonic development
… development of embryos derived from sperm and spermatids, immature precursors of sperm was compared. Sperm-derived embryos developed significantly better than spermatid-derived embryos. Further research aiming to identify the reasons for the developmental advantage of sperm led to the …
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A Novel Collapsing Response Mediator Protein 2 Binding Domain for Kinesin Light Chain 3
… kinesin light chain expressed in elongating spermatids that develop the flagellum. In this study, we investigated the expression of CRMP2 in mouse testis and we studied if CRMP2 and KLC3 interact. We observed CRMP2 mRNA expression in testis, along with expression of the other family members …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… undergo two meiotic divisions to form haploid spermatids. Haploid spermatids differentiate through the elongation phase and eventually form mature spermatozoa through dramatic morphological changes termed spermiogenesis, during which: 1) the Golgi apparatus forms the acrosome, 2) nuclear …
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Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation
… viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and suspended cells. These two cell populations were …
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DEVELOPMENT OF NOVEL BIOTECHNOLOGICAL APPROACHES FOR ACCELERATING GENETIC GAIN IN THE BOVINE
… a protocol for isolating immature bovine spermatids and evaluating their fertilization potential using micromanipulation techniques. This was followed by the optimization of a decellularization protocol to create a testicular biological scaffold that mimics the native tissue …
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Reproductive Morphology and Spermatogenesis in <em>Demanyssus gallinae</em> (Degeer) (Mesostigmata: Dermanyssidae)
… testicular arms) and formation of haploid (n=3) spermatids from haploid (n=3) spermatogonial cells is entirely mitotic. Spermatogenesis begins within one hour after protonymphal feeding and continues through adult ecdysis in normally fed individuals. Nutritional deficit causes cessation of …
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Regulating transcript fate through RNA elements in the 3' untranslated region
… the first wave of this progress from round spermatids to elongated spermatids occurs roughly from post-natal day 21 to post-natal day 35. Tightly regulated temporal control of translation is essential to proper sperm development. During spermiogenesis, chromatin becomes tightly wrapped …
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Physiological and Molecular Consequences of Large Y Chromosome Long Arm Deletions in Mice
… MF1XYRIIIqdel phenotype in developing haploid spermatids was performed. Cellular elutriation and fractionation techniques were employed to separate spermatids at different stages of maturation and isolate different subcellular compartments. Differences in the transcriptional profile between …
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Dynamic changes of TDP-43 in testicular and epididymal sperm
… was present in Stage VIII step 16 elongated spermatids using indirect immunofluorescence. My results indicated that TDP-43 is not present in step 16 elongated spermatids. At spermiation, spermatozoa are released into the lumen of the seminiferous tubule and are functionally immature; …
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In vitro studies on genotoxicity and gene expression in spermatogenic cells: mechanisms and assay development
… to identify spermatogonia, spermatocytes and spermatids. The genotoxins H2O2, doxorubicin, N-ethyl-N-nitrosourea, N-methyl-N-nitrosourea, 6-mercaptopurine, 5-bromodeoxyuridine, methyl methanesulphonate and ethyl methanesulphonate were investigated. Cells were cultured and treated with …
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Mechanisms and consequences of DNA damage, response and apoptosis in spermatozoa.
… were identified in human spermatozoa and murine spermatids using multicolour immunostaining with laser-scanning confocal microscopy (LSCM) and Western blots. Based on significantly increased DDR foci in spermatozoa of smoking men, but lacking DDR foci in response to in vitro challenge with H2O2, …
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Mechanisms and consequences of DNA damage, response and apoptosis in spermatozoa.
… were identified in human spermatozoa and murine spermatids using multicolour immunostaining with laser-scanning confocal microscopy (LSCM) and Western blots. Based on significantly increased DDR foci in spermatozoa of smoking men, but lacking DDR foci in response to in vitro challenge with H2O2, …
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SPAG16 is a Bifunctional Gene Regulating Male Fertility
… in specific regions within the nucleus of round spermatids. These nuclear sub-domains also contain SC35, a known marker of nuclear speckles enriched in pre-mRNA splicing factors. Putative interaction partners of SPAG16S are also shown to play critical roles in the peri-nuclear region during the …
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Functional Elucidation and Characterization of GPA2/GPB5 and Its Receptor LGR1 in the Mosquito, Aedes Aegypti
… important for flagellar formation in developing spermatids. Using RNA interference, downregulation of LGR1 led to sperm defects, such as shortened flagella. Moreover, newly emerged males were characterized with significantly less spermatozoa and rendered less fertile. The GPA2 and GPB5 subunits …
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The Reproductive Cycle of Sceloporus undulatus From Alabama Corresponding Plasma Steroid Levels and Comments on Reproductive Variation.
… concomitant increase in testis mass. Males had spermatids with a few transforming spermatozoa in their testes as they entered brumation. The longer warm season in Alabama permitted the males to begin spermiogenesis and spermiation earlier and to complete fall recrudescence later than Missouri S. …
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THE SPECIAL WALLS AROUND GAMETES IN CERATOPTERIS RICHARDII AND AULACOMNIUM PALUSTRE: USING IMMUNOCYTOCHEMISTRY TO EXPOSE STRUCTURE, FUNCTION, AND DEVELOPMENT
… to the AGP-filled matrix surrounding developing spermatids, developing eggs are bathed in (1,5)-α-L-arabinan pectins and not AGPs. Lastly, I examined the unique cell walls that are integral to sperm cell differentiation and release in both Ceratopteris and Aulacomnium. The preponderance of …
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Regulation of γ-Tubulin Ring Complex Recruitment in Drosophila
… but not to the mitochondrial derivatives of spermatids, demonstrating that γ-TuRC heterogeneity can exist even within the same cell at the same developmental stage. I have also found that differences between the centrosomal and testes-specific mitochondrial isoforms of the γ-TuRC tethering …
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Regulation of γ-Tubulin Ring Complex Recruitment in Drosophila
… but not to the mitochondrial derivatives of spermatids, demonstrating that γ-TuRC heterogeneity can exist even within the same cell at the same developmental stage. I have also found that differences between the centrosomal and testes-specific mitochondrial isoforms of the γ-TuRC tethering …
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