Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Sperm chromatin"”.
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Analysis of Human Sperm Chromatin Integrity
… present study evaluated the integrity of human spermatozoal nuclear material in the two condensation stages of chromatin and chromosomes. Original semen samples (ORI) and their swim-up fractions (SW, selected for motility) from men of known (donors) and unknown (patients) fertility were …
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Towards A Unified Model Of Sperm Chromatin Structure
<p>Sperm possess several layers of information that are delivered to the oocyte alongside the paternal DNA. Examples of potential sperm borne molecular cues of probable use to the embryo include RNAs and local and global chromatin structure. To identify candidate sperm RNAs that likely reach the …
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Evaluierung durchflusszytometrischer Verfahren zur Beurteilung der Qualität von kryokonserviertem Hengstsperma
… for assessing the quality of cryopreserved sperm from stallions. The plasma membrane integrity, the mitochondrial membrane potential, the acrosomal status and the integrity of the chromatin structure in cryopreserved sperm from stallions were assessed. For this purpose various fluorescent …
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Effects of Scrotal Insulation on Spermatozoal Morphology and Chromatin Stability to Acid Denaturation in the Bovine
The sperm chromatin structure assay (SCSA), as developed by Evenson et al.(1980), utilizes flow cytometry to quantify the susceptibility of sperm chromatin to in situ acid denaturation via the metachromatic properties of acridine orange. SCSA is repeatable and has been used to distinguish between …
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Negative biomarkers of sperm quality and male fertility
… light microscopy provides information about sperm motility and basic morphology but has a limited ability to predict reproductive performance in an assisted reproduction setting. In-depth analysis carried out quickly and with repeatable precision on a large number of sperm cells is important …
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Studies on Fertilization in Vitro in Mammals
… and electron microscopy, the behavior of the sperm-egg interaction: (a) during the normal fertilization process; and (b) when the fertilization process was modified chemically and physically. Chemical modification of the fertilization process entailed artificially fusing the sperm to the egg, …
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Studies on genetic aberrations as possible predictors of the outcome of assisted reproduction
… the World Health Organization (WHO) in regard to sperm concentration, motility and morphology are, however, poor predictors of fertility. In about half of the involuntarily childless couples the causes are solely or partly male related. In 60-75% of cases the aetiology of reduced semen quality …
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Biochemical and physiological changes in bovine sperm during extended incubation under aerobic and anaerobic conditions
… damage eventuating in the loss of fertility of sperm cells. This has been known for many years and current production systems for storage of semen in the liquid state attempt to minimise oxidative stress. This is achieved by reducing oxygen tension in the diluent through nitrogen gassing and by …
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Evaluation of Stallion Frozen-Thawed Semen Using Conventional and Flow Cytometric Assays
… of evaluating a large number of cells, including spermatozoa. Two experiments were designed to first validate the use of several flow cytometric tests on frozen-thawed stallion semen and then determine a model that may best explain variation in fertility. Comparing samples that were live and …