Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Spectral Flow Cytometry."”.
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Modeling of NK Cells in Pediatric Patients With Unusually Severe or Recurrent HSV Using High-Dimensional Flow Cytometry
… PLCγ2 phosphorylation status of NK cells using flow cytometry.</p> <p>This project required learning spectral flow and processing the results to analyze the high-dimensional data. We aimed to analyze an extensive immune profiling panel containing 19 colors for patients' PBMCs using spectral flow …
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Investigating the Reconstitution and Function of Peritoneal Cavity Macrophages after LPS-induced Peritonitis
… relevant dose of lipopolysaccharide. Spectral flow cytometry and confocal whole mount imaging was used to investigate the ‘disappearance’ of LPM from the peritoneal cavity upon stimulation of the noxious agent. After 10 days of LPS administration, the LPM fate mapping mouse and the …
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Killer Cell Immunoglobulin-Like Receptors Delineate Distinct Phenotypes and Functions in Human γδ T Cells
… (CMV+) healthy adult humans, using spectral flow cytometry. I found that CMV leaves a stable imprint in the γδ T cell repertoire and phenotype. CMV+ individuals have increased proportions of Vδ1 T cells within the total γδ T cell population. Moreover, γδ T cells from these …
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Low-dose IL-2 for T-regulatory cell expansion after alemtuzumab therapy in relapsing-remitting multiple sclerosis
… alemtuzumab therapy. Using quantitative flow cytometry and PhosFlow techniques, I showed that following alemtuzumab therapy, naïve CD4 Teff cells gained higher CD25 expression and became more sensitive to IL-2 *in vitro*, potentially narrowing the therapeutic gap. In mice treated with …
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Integrating Rehabilitation Mechanics and Immune Profiling to Inform Outcomes in Orthopaedic Trauma
… immune profiling was performed using flow cytometry to characterize systemic immune dynamics following injury and rehabilitation. Distinct temporal patterns in circulating immune cell populations—including monocytes, myeloid-derived suppressor cells, and T cell subsets—were observed …
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DISSECTING IMMUNE DYSREGULATION IN PATIENTS WITHTHYMIC EPITHELIAL TUMORS BY AUTOANTIBODY ANDSINGLE-CELL PROFILING
… high-throughput autoAbs screening, a 39-color spectral flow cytometry panel to deeply characterize αβ, γδ and MAIT compartments, and single-cell RNA sequencing paired with αβ TCR sequencing to reconstruct developmental trajectories, functional states and clonal relationships. Pediatric thymuses …
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The Impact of Heat Stress on Immune Function
… infection. This thesis combines in vivo models, flow cytometry and bioinformatics to investigate how heat stress influences haematopoiesis, immune function and susceptibility to viral infection. First, it was established that housing female mice at 36 °C was well tolerated over several weeks yet …