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Showing 1 to 14 of 14 for “"Spectinomycin"”.
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Biosynthetic Studies on the Aminocyclitol Antibiotics Neomycin and Spectinomycin
Made available in DSpace on 2014-12-13T20:09:51Z (GMT). No. of bitstreams: 1 7616202.pdf: 6067973 bytes, checksum: 761725ce8cc7bf6251fa6a436d7e84d3 (MD5) Previous issue date: 1976
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Characterizing and Overcoming Resistance to Aminomethylspectinomycins in Gram-negative Bacteria
<p>Spectinomycin (SPC) is a broad-spectrum aminocyclitol antibiotic. Its use in agriculture has led to widespread resistance in enteric bacteria, necessitating the development of more effective analogs. Aminomethyl spectinomycins (amSPC) are modified spectinomycins with increased potency against …
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Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… observed due to a high natural resistance to spectinomycin and streptomycin in rapeseed. In sugarbeet spectinomycin at a concentration of 100 mg/l was found efficient for selection and spectinomycin and streptomycin resistant colonies were obtained after callus bombardment. The presence of the …
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Understanding the intrinsic antibiotic resistance in Lysobacter enzymogenes, a ubiquitous environmental bacterium
… 12 and 19 mutants with decreased ampicillin and spectinomycin resistance, respectively. Further characterization of selected mutants showed that cell permeability, β-lactamase activity and transport contributed to high level of resistance to ampicillin in LeC3; whereas high level of resistance to …
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Pharmacokinetic and Pharmacodynamic Studies of a Novel Spectinamide Series of Antituberculosis Agents
… are novel amide derivatives of the antibiotic spectinomycin that have emerged as a new class of agents to treat tuberculosis. These agents showed potent <em>in vitro </em>activity against <em>Mycobacterium tuberculosis</em> (MTB)<em> </em>compared to spectinomycin and in a preliminary <em>in …
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Optimization of Lead Spectinamide Compounds as Novel Anti-tuberculosis Agents with a Pharmacometric Approach
… spectinamides – semi-synthetic analogs of spectinomycin. Spectinamides are a potent inhibitor of mycobacterial ribosomes and overcome efflux mediated drug resistance in M. tb. Spectinamides have shown an excellent in vitro activity, which makes them well suited for further lead optimization …
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Geographical Incidence of Antimicrobial Resistant Gonorrhea
… of treatment; sulfonamides, penicillin’s, spectinomycin, tetracycline, quinolones, and other macrolides, have once succeeded at curing gonorrhea. However, once resistance is built, gonorrhea then becomes untreatable. Currently, there are only two medications recommended in the treatment of …
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Expression Of Hepatitis C Viral Non-structural 3 Antigen In Transgenic Chloroplasts
… the 16S rRNA promoter, aadA gene coding for the spectinomycin selectable marker, psbA 5' untranslated region to enhance translation in the light and 3' untranslated region for transcript stability and trnI & trnA homologous flanking sequences for site specific integration into the chloroplast …
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Repurposing chlorpromazine and its metabolites for antituberculosis drug discovery
… defined as synergistic. A combination of M2 and spectinomycin (SPEC) exhibited the highest synergism against Msm (FICI 0.19) and Mtb (FICI 0.13). In vitro assays established that CPZ and M2 are bactericidal against Mtb whereas M3 and M5 are bacteriostatic on their own. In combination assays, the …
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The Role of Base Modifications on Tyrosyl-tRNA Structure, Stability, and Function in Bacillus subtilis and Bacillus anthracis
… The loss of ms2i6A37 conferred resistance to spectinomycin and ciprofloxacin, whereas the loss of Q34 resulted in sensitivity to erythromycin. Changes in the ratio full-length to truncated transcripts of the tyrS1 and tyrS2 genes were used to monitor tRNA mediated transcriptional regulation. …
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Optimisation of use of waste materials by Rhodopseudomonas palustris
… gene is a useful selection marker, but that spectinomycin and its resistance gene are less effective. A combination of promoter and ribosome binding site is reported that directs a high level of gene expression; this is a useful new tool for future genetic engineering attempts. Finally, the …
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PKPD-Model Based Preclinical Characterization of the Activity of Spectinamide 1599 Against Mycobacterial Subpopulations. and the Identification of Potential Partners for Combination Therapy
… Spectinamide 1599 is a synthetic modification of spectinomycin that has demonstrated excellent preclinical drug like properties such as excellent in vivo efficacy, strong efficacy against clinical MDR isolates, lack of cross resistance, long post antibiotic effect, and low toxicity potential. It …
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Expression Of Gal/galnac Lectin Of Entamoeba Histolytica In Transgenic Chloroplasts To Develop A Vaccine For Amebiasis
… regulate transcription. The aadA gene conferring spectinomycin resistance has been used for selection and gene10 regulatory sequence from T7 bacteriophage to enhance translation. The chloroplast integration of LecA was confirmed by PCR and Southern blot analysis. The expression of LecA protein in …
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Transformation der Plastiden und Mitochondrien bei höheren Pflanzen - Selektive Marker und Einsatzmöglichkeiten
… Erhöhung des Lysingehaltes- Es konnten 34 Spectinomycin-resistente Tabakklone selektiert werden, die mit der Transformationskassette pHoDh2b transformiert wurden. Die molekularbiologische Charakterisierung der Transformanten konnte bei 8 Linien eindeutig eine korrekte plastidäre Integration …