Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"Spatial proteomics"”.
-
Bayesian Methods for Spatial Proteomics
… a dozen subcellular compartments. To analyse spatial proteomics data, they are first annotated with marker proteins, which are proteins with a priori known unambiguous localisations. Typical analysis proceeds by training a machine learning classifier to assigned proteins with unknown …
-
Statistical Inference and Community Detection in Proximity and Spatial Proteomics: Resolving the Organization of the Neuronal Proteome
… advances in protein mass spectrometry (MS), aka proteomics, haveenabled high-throughput quantification of spatially-resolved, subcellular-specific proteomes. Biological insight in these experiments depends upon sound statistical analysis. Despite the myriad of existing proprietary and open-source …
-
Multiplexed, scalable, and functionality compatible platforms for 3D spatially resolved proteomic profiling
… it is necessary to characterize the spatial distribution and molecular functionality of biomolecules within the context of their local microenvironment and their interactive role in the whole biomolecular network. Spatial proteomics serves as an indispensable tool for extracting and …
-
The role of recombinant human laminin-111 in adhesion-signaling and glycosylation in laminin-⍺2 deficient muscle
… need to develop therapeutics for LAMA2-CMD. Spatial proteomics analysis of LAMA2-CMD patient biopsies revealed altered biological processes including protein aggregation, oxidative stress, and dysregulated glycolysis. In the LAMA2-CMD mouse model, the dyW, spatial proteomics showed similar …
-
Generative modeling of the tumor microenvironment : deconvolution, completion, and integration
… diverse views of tissue state, including spatial transcriptomics, spatial proteomics, and multi-sequence medical imaging. However, the data collected by these platforms is often incomplete, aggregated, or distributed across unpaired modalities. These acquisition constraints create three …
-
TRYPTOPHAN-DERIVED MICROBIAL METABOLITES PROMOTE NKT22 PRO-TUMORIGENIC FUNCTIONS IN COLITIS ASSOCIATED COLORECTAL CANCER PATIENTS
… single-cell cytometry, metagenomics, RNAseq, spatial immunophenotyping, transcriptomics, ex vivo and in vitro experiments were conducted. Metabolomics identified microbial-derived molecules activating iNKT cells. Mechanisms were studied using CAC murine models with or without functional iNKT …
-
Chemokine Ligand-3 (CCL3) as a Novel Mediator of Inflammatory Bowel Disease Activity
… cell type expression and CCL3-expressing cell spatial relationships. Immunohistochemistry found that there was no correlation between the severity of inflammation and percent of CCL3 positive cells in the lamina propria of IBD patient gut mucosa biopsies. However, IMC analysis highlighted a …
-
Enzyme engineering by directed evolution and applications in cellular imaging and proteomic mapping
… the Ting lab used for electron microscopy and spatial proteomics. This led to the successful discovery and characterization of the highly-active APEX2 which enabled advancements in mitochondrial and mitochondria-endoplasmic reticulum junction biology.
-
Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
… High-throughput expression and subcellular spatial proteomics were then applied to profile the response of HEK293-VP 2.0 cells to rAAV production. The effect of rAAV production on global protein abundance was determined by quantifying HEK293-VP 2.0 cell proteins in mock transfected and …
-
Developing and utilizing a robust mass spectrometry-based approach to study glucose metabolism and transport in the lens
… within the lens, but also develops a highly spatially resolved methodology to be able to study this. Firstly, I optimised a paradigm that would allow Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) analysis of glucose uptake and metabolism in ocular lenses. …
-
Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
… their widespread use, little is known about the spatial proteomic landscape of these industrially important cells, which might reveal information about protein location and protein-protein interactions pertinent to mAb secretion and molecular bottlenecks. This thesis details the cell …