Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Sox17"”.
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The Role of Sox17 in Normal and Pathological Beta Cell
… of this process can result in diabetes. Sox17 is a transcription factor and a key regulator in various developmental and disease contexts. During endoderm development, Sox17 acts, in part, by transcriptionally regulating other important endodermal transcription factors including HNF1β and …
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Biochemical and Functional Investigations into the Contribution of SOX17 Mutations to the Pathogenesis of Pulmonary Arterial Hypertension
… of patients, rare heterozygous variants in the SOX17 gene, predicted to be functionally deleterious, were significantly associated with PAH. SOX17 is an essential transcription factor involved in the development of the pulmonary vasculature and is highly expressed in endothelial cells. The aims …
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Genesis of the human germline
… Using the CRISPR toolkit, I further show that SOX17 enhancers are the critical targets of the regulatory circuitry of germline competence. SOX17 and TFAP2C initiate a core germline program, including induction of PRDM1 by SOX17 and maintenance of pluripotency factors, POU5F1 and NANOG. I also …
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The role of pou2/spiel-ohne-grenzen (spg) in brain and endoderm development of the zebrafish, Danio rerio
… of the endodermal differentiation marker sox17 in a mutually dependent way, and that the ability of Cas to ectopically induce sox17 strictly requires Pou2. I conclude that both maternal and zygotic pou2 function is necessary for commitment of endodermal progenitor cells to differentiate …
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WNT signalling in the patterning of human gastruloids
… Brachyury expression, whereas lower favour SOX17 expression, triggering the morphogenesis of SOX17+ tube-like structures. Through these experiments, it is further revealed that human gastruloids might recapitulate the mode of endoderm specification recently observed in mouse embryos. …
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Role of Sonic Hedgehog in Development of the Central Nervous System
… of meso-endoderm lineage markers, Gsc, Sox17, and GATA4. The results suggest that Shh promotes neurogenesis in multiple ways: by inducing the neuroectodermal cells, by repressing non-neural cell fates, and by promoting differentiation of neurally committed progenitor cells into motor and …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… and within each population. Within the SOX17+ population, the DE markers CER1 and FOXA2 were co-expressed in the majority cells independent of activin A treatment. By contrast, HHEX, CXCR4, FOXA2, MIXL1 and LIM1/LHX1 were expressed to various extents within the SOX17+ populations of each …
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Finding the Best Gene Candidate(s) as a Potential Therapeutic Target for N-MYC Overexpressing Neuroendocrine Prostate Cancer (NEPC)
… focus on validating three candidate genes (WNT1, SOX17, and FOXN1) identified from the genome-wide screen. Moreover, to suggest a more effective strategy to treat NEPC, we applied a combination of gene knockout and drug therapy. Three drugs, Chidamide, Belinostat, and Fludarabine, were tested to …
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Development of 3D multicellular liver organoids derived from human induced pluripotent stem cells to model antiretroviral therapy induced liver injury
… Nanog, and Sox2) and the definitive endoderm (SOX17 and GSC) markers. Flow cytometry using EpCAM, CD166 and CD68 antibodies indicated that HLOs comprise of 60,4% EpCAM+ cells, 11,2% EpCAM-/CD166+ cells and 5% EpCAM-/CD68+ cells, respectively. HLOs exhibited high CYP3A4 enzyme activity compared …
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The Role of BMP9-Induced SEMA3G in Pulmonary Vascular Stability
… Smad signalling pathway. Involvement of SOX17 in *SEMA3G* upregulation was also observed. Additionally, *Sema3g* expression was reduced in *Bmp9* knockout mice and in an experimental animal model of PAH. Interestingly, VEGF treatment inhibited the BMP9-mediated increase of *SEMA3G* …
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Synthesising executable gene regulatory networks in haematopoiesis from single-cell gene expression data
… experimentally, demonstrating that HoxB4 and Sox17 directly regulate the haematopoietic factor Erg, and that Sox7 blocks primitive erythroid development. A general-purpose graphical tool was then developed based on this algorithm, which can be used by biological researchers as new single-cell …
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Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
… this model, I identified an inhibitory role for SOX17 in anterior hypoblast specification. Modulation of the subpopulations of hypoblast-like cells impacted epiblast-like domain differentiation, highlighting functional tissue crosstalk. Overall, this Thesis begins to illuminate the ‘black box’ of …
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The role of transcription factor GATA6 in the development of the human pancreas
… with key definitive endoderm markers CXCR4 and SOX17, and pancreatic marker PDX1. Taken together, the work herein demonstrates the successful use of hPSCs coupled with the TALEN genome editing technology as a unique in vitro system for disease modelling. These findings also establish two …
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Signalling and transcriptional regulation of human primordial germ cell specification
… networks in mouse and human PGCs. For example, SOX17 is essential for human germ cell fate, but is dispensable for mouse PGCs. On the other hand, a key pluripotency factor PRDM14 is crucial for mouse PGC specification, but its role in human PGC development is unclear. I designed genetic …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… endoderm. Markers for the mesoderm and endoderm: SOX17, GATA6, Bra, and MIXL1 was expressed in varying levels while only gastruloids formed with a bone morphogenetic protein-4 signaling center, expressed markers for anterior neural lineages as seen by an upregulation in POU3F1, EN1, OTX2, IRX6, …
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A Translational Approach to Modeling Unique Aspects of Germ Cell Development During Self-Organization of the Primate Embryo
… and early ectodermal fate) and upregulate SOX17, which has been previously placed upstream of BLIMP1. Finally, we propose the marmoset as a good model of primate reproduction and embryogenesis, in an attempt to describe a non-human primate (NHP) system for validation of hPGC characteristics …
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Deep forward and reverse phenotyping for genetic discovery in pulmonary arterial hypertension.
… TBX4 (high impact), EIF2AK4 (biallelic) and SOX17 had a significantly younger age of disease onset (tag: young age). Patients with familial pulmonary arterial hypertension were harbouring deleterious variants in AQP1 (log(BF) = 10.023, PP = 0.958). Additionally, high impact variants in BMPR2 …
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Morphologisch und Molekular studien der Keimblätter Differenzierung im frühen Saüger Embryo
In den Säugetieren Embryos sind auch Keimblätter und deren Vorstufen für die Sortierung der Vielzahl von Zellen des frühen Embryos in genau definierten Fächer und für die Zurückverfolgung Zell-und Gewebekulturen Schicksale nützlich für bestimmte Organe oder Gewebe Fächer bestimmt. Jedoch viele …