Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Southern hybridization"”.
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A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin
… polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with southern hybridization data proved superior to both types of PCR based methods. PFGE produced distinct banding patterns that were easily interpreted both visually and with Molecular Analyst software. …
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Plasmid Structure and Function in Plant-Pathogenic Pseudomonas Syringae (outer-Membrane Proteins)
… by restriction endonuclease digestions and Southern hybridization. There is also extensive homology among plasmids indigenous to the oat-specific Pseudomonas syringae pv. coronafiens and pv. striafaciens strains. However, some of the multiple plasmids within an individual strain are …
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Pathogenicity Genes of Pseudomonas Syringae Pv. Tabaci Br2
… mutants failed to produce tabtoxin. Southern hybridization analysis using ('32)P-labelled pGS9 indicated that Tn5 had inserted into different EcoRI and SalI restriction fragments in each of the eight nonpathogenic mutants. Hybridization to additional EcoRI and SalI bands in two …
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Stress response of porphyromonas gingivalis
… other black-pigmented Bacteroides strains by Southern hybridization. DnaK and GroEL homolog proteins and some other proteins were also induced when P. gingivalis cells were challenged by ethanol. Exposure to oxidative stress and an elevation or decrease in pH did not show a discernible …
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Molecular cloning, characterization, and expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase gene from tomato
… HMGR sequences from different species. Genomic Southern hybridization analyses reveals that tomato contains 3 to 4 HMGR genes. The HMGz2 gene cross-hybridizes to mRNA of about 2.7 kb which is highly induced in tomato cells treated with fungal elicitors and in stems, leaves, or roots stressed by …
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Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant
… 06 appeared to contain two respectively from Southern hybridization experiments using a probe for left transposon junction. However, when a probe to right transposon junction was used, the chromosomal deoxyribonucleic acid (DNA) of mutant m5 was shown to contain approximately eight junction …
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cDNA Cloning and Gene Characterization of Large and Small Subunits of Ribonucleotide Reductase in Soybean
… specific for the RNR large subunits in plants. Southern hybridization results imply that a gene family encodes at least three different large subunits of RNR in soybean, and that a single gene encodes the small subunit. The presence of three different large subunits of RNR in soybean suggests …
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Generation of Transgenic <i>Medicago Sativa</i> Overexpressing "<i>Osmotin-Chitinase</i>" Gene Chimera
… positive transgenics was performed using PCR. Southern hybridization was carried out for further confirmation of successful transformation. Transformed shoots will be grown on the root inducing medium for developing into plantlets which would then be transferred to the green house and later …
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Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides
… Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. Strains were constructed in which one or two rRNA operons were deleted and the effects on cell growth …
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Molecular-cytogenetic analysis of repetitive sequences in genomes of Beta species and hybrids
… high-resolution multicolor fluorescent in situ hybridization (FISH) of the satellite and dispersed repetitive sequences in wild and cultivated beet species and in their hybrids. New repetitive sequences were isolated from the B. procumbens genome. The AluI restriction satellite repeats pAp11 are …
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Investigation of the Substrate Recognition Characteristics and Kinetics of Mammalian Mitochondrial DNA Topoisomerase I
… and pZT-400) served as control substrates. Southern hybridization using probes specific for either control or mtDNA-containing plasmids indicated a relative preference by the mitochondrial topoisomerase I to relax supercoils in pZT-Hori and pZT-Term. Quantitative determination of kinetic …
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A study of chemolithoautotrophic bacterial rhodanese, and its potential contribution to cyanide wastage during cyanidation of biooxidized concentrates
… isolate a rhodanese gene from T. caldus MNG by Southern hybridization, using the Azotobacter vinelandii rhdA gene as a probe, was unsuccessful. On balance of the information available from this study, and reported elsewhere, rhodanese activity probably does not contribute as much to thiocyanate …
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Cloning and expression of cambialistic Bacteroides fragilis superoxide dismutase gene
… DNAs were selected based on he results of plaque hybridization. Further analysis with restriction mapping and DNA sequencing revealed that only one recombinant phage DNA contained the SOD gene. Southern hybridization and restriction mapping located the SOD gene in the SalI-BamHI fragment (2.1 kb). …
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Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients
… been significantly improved barring the need for southern hybridization. This improved methodology was employed for the molecular typing of MAC in 100 resected, full-thickness tissue samples removed from IBD patients. The tissue samples were homogenized, decontaminated, and inoculated into two …
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Investigation of the role of the extracellular β-agarase, produced by the bacterial epiphyte Pseudoalteromonas sp. LS2i, in the virulence response towards the agarophyte Gracilaria gracilis
… that both clones contain the same agarase gene. Southern hybridization studies confirmed the origin of the cloned DNA and sequencing studies revealed the 1062 bp ORF, putative promoter region, putative ribosome binding site and putative transcriptional start point of the cloned agarase gene. The …
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Expression of Human Protein C in Transgenic Tobacco
… transgene copy number were determined by genomic Southern hybridization and segregation analyses. The majority of transgenic plants expressed the hPC transgene based on RNA analyses by northern hybridization. Plants utilizing the enhanced 35S promoter had equivalent levels of expression to …
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Molecular Studies of Restriction Genes in Enteric Bacteria
… was constructed in this study using PFGE and Southern hybridization. The genome was digested with three rare cutting restriction enzymes (<em>BlnI, I-Cuel</em> and <em>XBal</em>). The estimated size of the GM236 genome was 4,582 (±80) kb, in accordance with the range of other enteric bacteria. …
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Studies on improvement of Chinese wheat cultivars for resistance against an endemic virus using transgenesis and virus-wheat interactions at the molecular and ultrastructural levels
… the regenerated wheat plants was demonstrated by Southern hybridization analysis. Expression of viral CP gene was confirmed from most CP-transgenic wheat by RT-PCR testing, while only one CP-transgenic plant produced CP at a detectable level. The meiotic stable transmission of transgenes in …
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Rhamnose metabolism and metronidazole resistance in B. thetaiotaomicron VPI-5482
… a MIC = 8 μg/ml was isolated and characterized. Southern hybridization was used to identify the insertion of a single copy of the transposon in an intergenic region of a gene cluster involved in the uptake and catabolism of L-rhamnose. RNA hybridization studies confirmed that the genes in B. …
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Molecular Cloning and Analysis of the Modification Genes of the KpnBl Restriction- Modification System of Klebsiella pneumoniae Strain GM236
… size. The use of these clones in future Southern hybridization tests will help to complete the restriction map and allow the accurate mapping of the KpnBl R-M genes on the chromosomal map of GM236.</p>
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