Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Somitogenesis"”.
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A genetic analysis of somitogenesis in the Medaka (Oryzias latipes)
… into skeletal muscle, cartilage and dermis. Somitogenesis is regulated by a complex interplay of different pathways. Notch/Delta signaling is one of the pathways well characterized in zebrafish through mutants affected in its different components. Previous work in mouse, chicken and zebrafish …
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Investigation of cell movement and the associated cytoskeleton during chick gastrulation and somitogenesis
… cytoskeleton) during chick gastrulation and somitogenesis. The aims of this project were to a) further characterise the cytoskeleton in cells that ingress into the avian primitive streak. b) Establish a published electroporation technique, which permits the targeting of different regions of …
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The evolution of somitogenesis: Mechanisms of paraxial mesoderm elongation in zebrafish and other vertebrates
In vertebrate embryos, a process called somitogenesis lays the foundations of the adult spine. This process involves elongation and segmentation of the paraxial mesoderm to form somites. Although the segmentation aspect of this has been widely studied, the elongation aspect is not well understood. …
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Single Cell Methods to Learn Transcription Factor Interactions and Necessary Noncoding DNA During Zebrafish Somitogenesis
… their sufficiency and necessity during zebrafish somitogenesis. This framework offers a low-cost and scalable approach to decode the regulatory logic of development and provides a blueprint for functionally annotating the noncoding genomes of multicellular models.
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Molecular and biochemical characterisation of phosphorylation-dependent NICD1 turnover: novel roles for CDK1 and CDK2
… presomitic mesoderm (PSM). The rhythmicity of somitogenesis is regulated by a molecular clock called the segmentation clock that drives the periodic expression of a number of “clock” genes in the PSM, with a periodicity that matches somite formation. Clock genes belong to the Notch, Wnt and FGF …
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Analysis of the Zebrafish Segmentation Clock
… presomitic mesoderm (PSM) by a process called somitogenesis. <br></br><br></br> Underlying the periodicity of somitogenesis are transcriptional oscillations of cyclic genes in the cells of the PSM. On a tissue level, these oscillations manifest as travelling waves, departing from the posterior …
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The Regulation of Segmentation Clock Period in Zebrafish
… of growing tissues, as is the case in vertebrate somitogenesis, and potentially also in vertebrate limb outgrowth and axial segmentation of invertebrate embryos. During vertebrate segmentation, the embryonic axis is subdivided along its anterior-posterior axis into epithelial spheres of cells …
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Dact Family Molecules In Wnt Signaling In Mouse Development
… of this gene in patterns suggesting roles in somitogenesis and neuronogenesis, Dact1 mutant mice are caudally truncated due to defective mesoderm formation in late gastrulation. Dact1 mutant mice show reduced Wnt/β-catenin signaling. The Dact1 mutation and the mouse planar cell polarity mutant …
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A novel genome-wide approach to identify in vivo targets of Notch signalling
… of Notch signalling and key regulator of somitogenesis, was similarly generated. Although the tagged Hes7 protein is functional and gets biotinylated in cell culture assays, the transgenic mice exhibit a severe somite/skeletal phenotype indicating that the tagged allele is hypomorphic. A …
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Novel Notch signalling dynamics within the molecular oscillator underlying vertebrate segmentation
… are derived. Produced during the process of somitogenesis, somites periodically bud off in pairs with strict, species-specific periodicity from an area of unsegmented tissue in the caudal end of the embryo termed the pre-somitic mesoderm (PSM). This periodicity is regulated by a molecular …
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Assessing the transition state model for cellular differentiation in vivo: a case study of zebrafish neuromesodermal progenitors
… cord or paraxial mesodermal lineages at the late somitogenesis stages, in the absence of other cellular processes such as proliferation, apoptosis and extensive mixing. Thus, I was able to relate cell states to cell fates as I analysed the gene expression heterogeneities in the NMp population in …
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Transcriptional divergence during cichlid embryogenesis
… covering four developmental stages: gastrula, somitogenesis, pharyngula, and larval. The single-cell profiles also highlight early divergence in gene expression, with genes showing species-specific expression patterns predominantly expressed during the gastrula and somitogenesis stages. These …
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Mechanisms of brain ventricle development
… rapidly, over a four-hour window during mid-somitogenesis. In order to determine the genetic mechanisms that affect brain ventricle development, I studied 17 mutants previously-identified as having embryonic brain morphology defects and identified 3 additional brain ventricle mutants in a …
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Characterisation of the gene regulatory network for posterior segmentation in Parasteatoda tepidariorum
… in an analogous mechanism to that regulating somitogenesis in vertebrates. However, it remained unknown how the two major signaling pathways interact during the formation of the SAZ and regulate other putatively downstream segmentation genes, such as evenskipped (Pt-eve) and runt (Pt-run-1). …
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Role of Xenopus Pitx3 during early development.
… indicate that xPitx3 may also play a role in somitogenesis through modulating Hox-1A and hairy2 genes that are involved in spatiotemporal specification of developing somites. Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0692. Adviser: Michael Crawford. Thesis …
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A multi-lineage in vitro mouse embryo model derived exclusively from mouse embryonic stem cells
… body axis formation and aspects of gastrulation, somitogenesis, cardiogenesis, and neurulation. However, it fails to recapitulate the spatiotemporal interplay of signalling pathways between embryonic and extraembryonic tissues. Another approach is to combine ESCs with extraembryonic stem cells …
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Roles for Chd7 in Zebrafish Development with Implications for Human Disease
… in laterality defects in the expression of somitogenesis genes, consistent with the hypothesis that this chromatin remodeler is necessary for the proper development of the long axis of the body and may be involved in the onset of human scoliosis. We show that the presence of Chd7 is crucial …
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Pattern Formation During Extensive Cell Movements
As embryos develop, they are required to generate organised patterns of gene expression across developing tissues. Previous hypotheses used to explain how patterns form in developing tissues have relied on the ideas of positional information and gradients of signalling across the developing tissue, …
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Redefining the Gastrula Organiser in Early Chick Embryo Development
… progenitors to become node tissue, delaying somitogenesis onset and extension of the closely associated axial tissues.
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Identifizierung und Charakterisierung von Genen für die Entwicklung der Nieren und des Urogenitalsystems
Das Studium der Nierenentwicklung gibt Einblicke in generelle entwicklungsbiologische Prozesse wie induktive Wechselwirkungen, mesenchymale Kondensation, mesenchymale-epitheliale Umformung, Determinierung von Zellschicksal sowie Differenzierung und damit auch in die Entstehung congenitaler …