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Showing 1 to 20 of 23 for “"Somatic embryogenesis"”.
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Somatic embryogenesis in Pinus radiata
Somatic embryogenesis of Pinus radiata has been seen as an attractive alternative to traditional propagation methods. This is due to its potential for high multiplication rates and rejuvenation, as well as its compatibitity with molecular techniques. To date no commercial production of somatic …
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PROPAGATION OF PLANTS BY SOMATIC EMBRYOGENESIS
… would be reduced by utilising the phenomenon of somatic embryogenesis for plant regeneration (Jones, 1984, Biochem. Soc. Symp. 98: 222-232). Although many publications describe how mbryogenesis may be induced in particular cases (reviewed by Williams and Maheswaran, 1986, Ann. Bot. 57: 449-462), …
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MOLECULAR ASPECTS OF DIRECT SOMATIC EMBRYOGENESIS IN ALFALFA (MEDICAGO)
Somatic embryogenesis follows the developmental pattern of the zygote in vitro so it provides a model for early plant development which is more accessible for experimental manipulations. Most of the fundamental research in this area has exploited indirect embryogenic systems which require cells to …
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Propagation of asparagus (Asparagus officinalis L.) via somatic embryogenesis
This thesis reports the development of somatic embryogenesis system for asparagus (Asparagus officinalis L.). Somatic embryogenesis is important as an efficient system for clonal propagation. To achieve this technique, the first step is the initiation of callus followed by the induction of the …
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Somatic Embryogenesis of Pathogen-Free Horseradish (Armoracia Rusticana) Plants in Vitro
… to produce whole plants from single cells via somatic embryogenesis. To initiate somatic embryogenesis, the Illinois Horseradish 'Doll' cultivar ('Doll') was tested with 2,4-D (0.25 to 1.0 mg/l) and BA in vitro. Somatic embryogenic callus was successfully induced from leaf explants on MS medium …
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Somatic embryogenesis of pathogen- free horseradish (Armoracia Rusticana) plants in vitro
… to produce whole plants from single cells via somatic embryogenesis. To initiate somatic embryogenesis, the Illinois Horseradish ‘Doll’ cultivar (‘Doll’) was tested with 2,4 -D (0.25 to 1.0 mg/l) and BA in vitro. Somatic embryogenic callus was successfully induced from leaf explants on MS …
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The identification and characterisation of key genes involved in somatic embryogenesis in wheat (Triticum, aestivum L.)
… embryonic development, via a process known as `somatic embryogenesis' (SE). On removal of auxin these totipotent cells are then capable of re-differentiating and regenerating into full adult plants. Although well characterised physiologically, the molecular mechanisms that regulate this …
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Somatic embryogenesis in southern and tropical pine species: Loblolly pine (Pinus taeda), Longleaf pine (P. palustris) and Oocarpa pine (P. oocarpa)
… establish an improved and reliable protocol for somatic embryogenesis in 1) Pinus taeda and Pinus palustris; pine species of high value for commercial applications and germplasm conservation supported through breeding programs at The Virginia Department of Forestry (Chapter III); and 2) Pinus …
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The control of embryogenic competence in Alfalfa (Medicago falcata L.)
Somatic embryogenesis is a process by which somatic cells undergo a developmental sequence similar to that observed in zygotic embryos. This process is not only an important plant propagation technique, but it also provides an essential tool for basic research into plant embryo development and …
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Regeneration and genetic transformation of rose and evergreen azalea
… regeneration systems via organogenesis and/or somatic embryogenesis, and develop gene transfer systems for these two important floral crops.
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Regeneration, DNA Methylation, and Transformation in Rosaceae
… protocol by taking advantage of a secondary somatic embryogenesis system was developed. Then, a microbial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated transformation. These lines were confirmed for stable integration of Ace-AMP1 and …
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Development of in vitro regeneration and gene transfer systems for conifer species
… of S. sempervirens, shoot organogenesis and somatic embryogenesis were induced. The influence of genotype and various cytokinins (BA, BPA, and TDZ) in combination with 2,4-D were investigated. Among the cytokinins tested, 20 $\mu$M BPA with 5 $\mu$M 2,4-D produced the highest frequency of …
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CELL DIVISION AND EMBRYOGENIC COMPETENCE IN ALFALFA {Medicago falcata L.)
… exploited a procedure for the production of somatic embryos directly from alfalfa leaf explants. Chopped leaf fragments were incubated in a liquid medium containing 2,4-D. The organisation of the swollen leaf tissue was disrupted and after 15 days the globular clusters of small dividing cells …
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Free radical mediated mechanisms in stress and development in in vitro crop plant systems
… was used as a model system for the study of somatic embryogenesis and for the development of an enzyme linked immunosorbant assay (ELISA) technique, for the detection of hydroxynonenal-protein adducts in plant extracts. This study shows for the first time, in plants, that exogenously applied …
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Applied Biotechnology for in Vitro Production of Carica Papaya L
… in the medium. A higher frequency of somatic embryogenesis was achieved in a shorter time in a liquid system than reported previously for other systems. The presence of abscisic acid (ABA) in the maturation medium enhanced embryo size and increased the percentage developing into …
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Development, selection and characterization of summer patch (Magnaporthe poae Landschoot and Jackson) resistant somaclones of Kentucky bluegrass (Poa pratensis L.) genotypes
To develop a somatic embryogenic system for Kentucky bluegrass, crowns, embryo axes, leaf, and endosperm tissues of twelve genotypes were cultured on Murashige and Skoog medium containing various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). Callus …
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Genetic Engineering of 2,4-D Resistance in Wine Grapes
… this goal a plant regeneration system based on somatic embryogenesis was developed for the 'Chancellor' wine grape. Secondly, an Agrobacterium transformation vector was constructed and its effectiveness demonstrated by the incorporation of the tfdA gene into tobacco. The vector was used to …
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