Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Soluble aggregates"”.
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The Role of Neuroinflammation in Alzheimer's Disease
… potential of synthetic amyloid beta 42 aggregates. In conjunction with a liposome-permeability assay and an immunoprecipitation assay, this assay has been used to validate a proprietary amyloid beta 42 antibody from MedImmune (now AstraZeneca). Most neuroinflammation studies have focused …
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Use of an Atg16l1 mouse model to study the role played by autophagy in neuroprotection
… revealed the accumulation of detergent in soluble aggregates of polyubiquitinated proteins in their central nervous system and in various other tissues. While polyubiquitinated aggregates are commonly associated with human neurodegenerative diseases, cell damage or accumulation of …
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Studying Models of the Amyloid Cascade Hypothesis Using Single-Molecule Imaging
… in multiple forms in the AD brain; while the insoluble plaques are the most studied species, the smaller, soluble aggregates may be more toxic, leading to neuroinflammation, oxidative stress, and tau phosphorylation. However, their small size and low abundance makes it challenging to study the …
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Cerebrovascular and Neuronal Cellular Consequences of Amyloid-β Induced Nuclear Factor-κB Activation
… disease. Mounting evidence suggests the small, soluble aggregates of Aβ induce the progressive neurodegeneration observed in AD patients. AD patients also display varying degrees of cerebrovascular pathology including the deposition of Aβ within vessel walls known as cerebral amyloid angiopathy …
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Growth and Toxicity of Amyloid β Polymorphs
… of the protein fragment amyloid β (Aβ) into soluble aggregates or insoluble β-sheet-rich fibrils and plaques in the brain. Limited success in targeting these fibrils and plaques stems from a lack of understanding of the intricate process of Aβ fibrillization. We investigated the unknown …
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Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in V. alginolyticus
… It was discovered that the protein forms soluble aggregates in the absence of reducing agent. Trypsin digestion coupled with mass spectrometry revealed the existence of Cys883/Cys892 disulfide bridge. Homology modeling and DALI server was used to show that Cys892 is fairly conserved in HPT …
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Single-molecule studies of alpha-synuclein and abeta in Parkinson’s disease derived biofluids
… disorders is deposits of fibrillar protein aggregates in the human brain. However, recent evidence suggests that the small soluble aggregates of alpha-synuclein (αS) and Abeta (Aβ), often referred to as oligomers, are more neurotoxic than mature fibrils. Thus, this work aims to study the …
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Influence of Protein Concentration on Heat-Induced Aggregation and Subsequent Functionality of Whey Proteins
… particle size (Dynamic light scattering, DLS) of aggregates was significantly (P < 0.05) affected by both protein concentration (1 to 12 % w/w) and pH (6.2, 6.7 and 7.2). Aggregates of ~ 50 nm were formed at higher pH (7.2) and protein concentration (12%), contributing to greater heat stability …
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Model-Based Measures Of Interrater Agreement
… 55 was also shown to inhibit the growth of soluble aggregates by both association and elongation inhibition. In addition, compound 56 which bears a 1,8-naphthalimide group connected to a 2-dimethylaminofuran group by a two-carbon linker demonstrated potent Aâ aggregation inhibitions (65.6% …
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Untersuchungen zum Einfrier- und Auftauverhalten pharmazeutischer Humanproteinlösungen im Großmaßstab
… Daclizumab showed a significant increase of aggregates after freeze-thaw-cycles in a PBS-buffer media. It could be demonstrated that the reason for the instability was the pH-shift of the freezing buffer. Replacing sodium phosphate with potassium phosphate stabilized the protein. The same …
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Molekulargenetische und reaktionskinetische Untersuchungen nativer und rekombinanter saccharolytischer Clostridien zur Optimierung der biologischen Wasserstoffproduktion
… revealed that the protein forms large soluble aggregates and for further biochemical characterisation its isoelectric point was determined. To optimise the biological hydrogen production of C. acetobutylicum genetically modified strains were developed. The genes coding for the …