Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Solid-state nuclear magnetic resonance (SSNMR)"”.
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Solid state nuclear magnetic resonance methodology for biomolecular structure determination
Several developments in solid state nuclear magnetic resonance (SSNMR) spectroscopy methods are presented. All studies are performed with magic angle spinning (MAS) and high-power proton decoupling, for optimal sensitivity and resolution. Chemical shift are assigned by multi-dimensional correlation …
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A short & sweet story of CHO
<p>The solid state nuclear magnetic resonance (SSNMR) study of nearly intact plant cell walls along with the computational study of glucose and malonic acid are presented. Previously unassigned <sup>13</sup>C chemical shifts are presented for the various carbohydrates in the primary plant cell wall …
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Methods for Increasing Sensitivity and Throughput of Solid-State NMR Spectroscopy of Pharmaceutical Solids
Solid-state nuclear magnetic resonance (SSNMR) spectroscopy has been demonstrated to be a powerful technique for investigating solid dosage formulations. SSNMR has the ability to determine physical form, molecular structure, and dynamics of a pure or formulated active pharmaceutical ingredient …
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Applications of DNP and solid-state NMR for protein structure determination
Magic Angle Spinning (MAS) solid state nuclear magnetic resonance (SSNMR) is a developing method for determining the structures and studying the dynamics and functions of biological molecules. This method is particularly important for systems, such as amyloidogenic fibrous proteins, that do not …
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Biomolecular Solid-State Nuclear Magnetic Resonance Methods for Spectral Assignment and High-Resolution Structure Determination of Proteins
Multidimensional solid-state nuclear magnetic resonance (SSNMR) experiments for the elucidation of structure in microcrystalline proteins are presented, utilizing the streptococcal beta-1 immunoglobulin binding domain of protein G (GB1) as a model protein. Complete 13C and 15N chemical shifts are …
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Investigating CO2 Adsorption Behavior in Metal-Organic Frameworks by Solid-state NMR
… and CO2 in MOFs is of fundamental importance. Solid-state nuclear magnetic resonance (SSNMR) is a powerful technique which reveals guest molecules’ behavior in MOFs. Herein, [Zn2(TRZ)2(NH2-BDC)] was loaded with pure CO2 and D2O, and CALF-20 was loaded with pure CO2 in this study. 2H and 13C …
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Use solid-state NMR to study the molecular structures of disease-associated peptide aggregates
Our lab uses solid-state nuclear magnetic resonance (ssNMR) to characterize the molecular structures of different types of aggregates formed by disease-associated proteins or peptides. In this thesis, we study three aggregate samples to reveal the structural information related to their molecular …
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Structural determinants of cardiac light chain amyloidosis
… We performed these studies through the use of solid-state nuclear magnetic resonance (SSNMR) spectroscopy, which have revealed new insights on specific features of the fibril species. First, we report our findings on the 13C and 15N chemical shift assignments and sequence involvement of AL-09 …
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Solid-state nuclear magnetic resonance investigations of the influenza M2 protein : structure and dynamics characterization of channel gating and conduction
Solid-state nuclear magnetic resonance (ssNMR) spectroscopy is a powerful technique that can be used to probe the structure and dynamics of biomolecules that are intractable to study by other structural biology methods. In this thesis, the mechanisms of conduction and gating are investigated in the …
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Purification and characterisation of novel recombinant β-glucosidases from aspergillus with application in biofuel production
… Thermogravimetric analysis (TGA) and 13Carbon Solid State Nuclear Magnetic Resonance (SSNMR) of pure BC before and after treatment with a commercially available Aspergillus cellulase enzyme was demonstrated. Two encoding sequences for novel Aspergillus nidulans hydrophobin genes ANID_05290.1 …
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Glycation of Extracellular Matrix: A Solid-State NMR Study
… matrix (ECM) and ribose-5-phosphate using solid-state nuclear magnetic resonance (SSNMR) spectroscopy. The background and motivations of this project are outlined in chapter 1. Chapter 2 reviews the current understanding of the composition and organisation of the ECM, the structure of …
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Structure and dynamics of membrane proteins from solid-state NMR
Solid-state nuclear magnetic resonance (SSNMR) spectroscopy is an essential tool to elucidate the structure, dynamics, and function of biomolecules. This thesis mainly focuses on the structure determination of the hydrophobic domains for fusion proteins which are involved in membrane fusion between …
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Characterization of structure and dynamics of membrane proteins from solid-state NMR
Solid-state nuclear magnetic resonance (ssNMR) spectroscopy is an essential tool for elucidating the structure, dynamics, and function of biomolecules. ssNMR is capable of studying membrane proteins in near-native lipid bilayers and is thus preferred over other biophysical techniques for …
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High field DNP and cryogenic MAS NMR : novel instrumentation and applications
Solid State Nuclear Magnetic Resonance (ssNMR) spectroscopy has blossomed over the last two decades. As ssNMR is progressively applied to more challenging systems, the sensitivity remains one of its major limiting factors. Gyrotron based high-field dynamic nuclear polarization (DNP) permits …
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Structural and Functional Studies on Cytochrome BO3 Ubiquinol Oxidase From Escherichia Coli and the Characterization of Its Quinone Binding Sites
… in quinone binding in subunit I. Electron paramagnetic resonance (EPR) spectroscopic studies were carried out to characterize the semiquinone at the QH site of cytochrome bo3. Three exchangeable protons were identified around the semiquinone. The data supported a model in which the semiquinone …
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New Tools for Structural Biology and Biophysics: High-Throughput Fluorine Solid-State NMR and Applications to Membrane Proteins
Solid-State Nuclear Magnetic Resonance (SSNMR) spectroscopy is a powerful method for characterizing the structure and dynamics of crystalline and amorphous solid compounds, materials, and biological systems. When applied to biomolecular systems such as membrane proteins, it can provide access to …
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Structure and dynamics of full-length M2 protein of influenza A virus from solid-state NMR
Solid-state nuclear magnetic resonance (SSNMR) has been frequently used to elucidate the structure and dynamics of membrane proteins and fibrils that are difficult to characterize by Xray crystallography or solution NMR. This thesis focuses on the structure determination and the proton conduction …
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Structure and dynamics of plant cell walls and membrane peptides from solid-state NMR
Solid-state nuclear magnetic resonance (SSNMR) is a powerful technique to study the structure, dynamics and interactions of bio-macromolecules. This thesis mainly focuses on the characterization of the architecture and loosening of primary plant cell walls and the interactions between membrane and …
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Molecular Analysis of Type IV Pilus Assembly in Clostridium perfringens
… understanding of the initial and final energetic states of the pilins will reveal insights into possible mechanisms of type IV pilus assembly. Toward that end, a pilin was purified from the Gram-negative pathogen Pseudomonas aeruginosa and incorporated into an artificial membrane. The pilin was …
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Investigation of cell interfaces and strained tissues with solid-state NMR
Solid-state nuclear magnetic resonance (ssNMR) is an immensely powerful tool for structural biology, which can provide insights into the composition, structure, dynamics of biological components. In combination with <sup>13</sup>C amino acid enrichment of cell fibroblasts *in vitro*, ssNMR has been …
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