Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Solanum phureja"”.
-
Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement
… by anther culture of a diplandrous genotype of Solanum phureja, a South-American diploid potato species, were examined for their utility in germplasm development. Nine monoploid genotypes and the diploid anther-donor plant were grown in photoperiod chambers at The Southeastern Plant Environment …
-
Factors affecting variability in anther culture and in regeneration of androgenic embryos of Solanum phureja
… for embryo production in anther culture of Solanum phureja was examined as a function of maximum greenhouse temperature prior to bud harvest and innate responsiveness among anthers within a bud. S. phureja clones PP5, AD2-4, A3P2-6 and AD3-4 were grown in a greenhouse under a 16 h …
-
Utilization of tissue culture methods and molecular markers for improvement of Solanum phureja Juz. & Buk.
Anther-derived monoploids of Solanum phureja were utilized to investigate factors essential to an efficient method of regenerating doubled monoploids (DMs). The presence of silver thiosulfate (STS) in the MS basal medium did not affect the frequency of cells with 2x nuclei but increased the …
-
Derivation, fertility and breeding value of doubled monoploids from the diploid potato species, Solanum phureja
… by anther culture of ten diploid clones of <i>Solanum phureja</i> were used to generate doubled monoploids through in vitro shoot regeneration. Doubled monoploids were compared to the anther donor and progenitor monoploids for morphological characteristics, and were evaluated for fertility in …
-
Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them
… of a diploid cultivated primitive potato, S. phureja (2n=2x=24). The parental pair consisted of AM3-8, an anther culture derived homozygous diploid, and NBP2, a heterozygous, field selected line. AM3-8 produced embryos in anther culture, and shoots on cultured leaf discs, but its cells did not …
-
Analysis of anther-derived plants of Solanum phureja: variation in ploidy, photosynthetic efficiency and structure of the nuclear genome
… exotic germplasm into existing cultivars of Solanum tuberosum through· 4x X 2x crosses using the South American diploid potato species Solanum phureja as the pollen parent. The first phase of this program includes the 'reconstruction' of a highly heterozygous diploid, pollen parent by .first …
-
The Potential for Green Fluorescent Protein as a Screening Tool in the Production of Haploid Potato Plants
… highly regenerative diploid clone (BARD 1-3) of Solanum phureja and haploid inducer IVP 101 was transformed with Agrobacterium tumefaciens strain 4404 containing plasmid pHB2892 with genes for green florescent protein (GFP) and kanamycin resistance. Hemizygous primary transformants (To) were …
-
Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families
… from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 …
-
Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum
… was studied in the diploid potato species, Solanum phureja. Based upon data from F₂ as well as from F₁ and backcross progenies, it was found that protoplast culturability in this potato species was controlled by two unlinked loci with dominant effect. In addition, there was quantitative …
-
Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… genomic constitutions but heavily favoring Solanum phureja, a primitive cultivated potato, were used in electrofusion experiments to create intermonoploid somatic hybrids (SH). The "monoploid sieve" results in the survival of only those gametes free of lethal and deleterious genes but …
-
Transposon Tagging in Strawberry and Potato and Characterization of Representative Strawberry Mutants
… of seeds and efficient transformation. Potato (Solanum tuberosum, 2n=4x=48) is an important vegetable crop in the world and is highly heterozygous. The successful sequencing of the homozygous doubled monoploid clone of potato provides good insight into the study of important genes in this …
-
Molecular marker analysis of a segregating monoploid potato family
… between high leptine producing selections of Solanum chacoense Bitt. and anther culture responsive selections of Solanum phureja Juz. et Buk. Several steps of the anther culture process were studied. The results indicated that genotype remains the main factor affecting anther culture response. …
-
AFLP Marker Analysis Of Monoploid Potato
… AFLP analysis was conducted on two sibling S. phureja "BARD 1-3" monoploid populations derived by androgenesis in anther culture, and gynogenesis through the use of a haploid-inducing pollinator, S. phureja "IVP 101." Little indication of somaclonal variation and haploid-inducer gene …
-
Bulk segregant analysis for anther culture response and leptine content in backcross families of diploid potato
… between a primitive cultivated species, <I>Solanum phureja</I>, and a weedy species, <I>S. chacoense</I>, were used to examine the segregation of microsatellite markers and three traits in backcrosses. Two of the traits, anther culture competence and 2<I>n</I> pollen production, originated …