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  1. The effect of iron metabolism on palmitate-induced INS-1 cell death

    Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 24 A. MATERIALS 24 1. Reagents 24 B. METHODS 25 1. Cell Culture 25 2. Preparation of palmitate 25 3. Isolation of islets 25 4. MTT-bases viability assay 26 5. Measurement of cell death 26 6. Western blot analysis 27 7. RNA extraction and microarray …

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