Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"Snake Venom"”.
-
Antigenic properties of snake venom cardiotoxins
In this dissertation an innitial investigation was made to lo~ate antigenic determinants of cardiotoxin vll 1 from N.nivea with a future view of applying the knowledge obtained in the design of synthetic vaccines. The conformation and surface properties of this toxin differs from that of proteins …
-
Crotalus Snake Venom Preconditioning to Prevent Surgical Brain Injury
… shows that preconditioning with Crotalus rattlesnake venom, known for its hemorrhagic and inflammatory effects, mitigates some harmful effects of SBI. We have identified two proteins of interest in Crotalus venom: snake venom metalloproteinase (SVMP), an enzyme with hemorrhagic effects, and …
-
Study of prothrombin activation by taipan snake venom using light beating spectroscopy
… and its activation kinetics via Taipan Snake Venom, micellar phospholipid and Ca++. The correlation times fCc) on the samples of human prothrombin, which were prepared by membrance filtering techniques, were measured as a function of scattering angle to determine the particle size …
-
CYTOTOXICITY MEDIATED BY L-AMINO ACID OXIDASE ISOLATED FROM THE VENOM OF CROTALUS MITCHELLII PYRRHUS
… of an L-amino acid oxidase isolated from snake venom in prostate cancer cell line. The enzyme causes oxidative stress, induces ER stress and activates autophagy in the cells in vitro, leading to the activation of apoptosis.
-
STRUCTURE, FUNCTION AND FOLDING OF THREE FINGER TOXINS
… (3FTxs) are one of the family of peptides in snake venom. Despite the common protein fold, they exhibit diverse pharmacological activities. Our group characterized Oh9-1, a neurotoxin from venom of Ophiophagus hannah. Although Oh9-1 binds to the same acetylcholine binding pocket as the …
-
The Oxidative Deamination of Sulfur Amino Acids by a Bacterial L-Amino Acid Oxidase
… to the presence of sulfhydrase. The crystalline snake venom L-amino acid oxidase from Crotalus terrificus terrificus was studied in comparison and found to catalyze a similar reaction.</p> <p>The pH optimum for each substrate was determined either by measuring the rate of oxygen consumption or …
-
Targeted Disruption of Lynx2 Reveals Distinct Functions for Lynx Homologues in Learning and Behavior
… as an endogenous mammalian homologue of snake venom peptide neurotoxins capable of binding to and functionally modulating nicotinic cetylcholine receptors (nAChR). Lynx1 is a member of the Ly6-α—neurotoxin superfamily (Ly6SF) of genes. Through extensive database searches, I identified 85 …
-
The Role of Reactive Oxygen Species in Crotalus atrox Venom-induced Cell Death
<p>Venomous snake bites impact humans all around the world. Anti-venom treatments mitigate systemic effects such as vascular hemorrhage, platelet aggregation inhibition, and the activation of inflammatory mediators. However, hemorrhagic snake venom also causes a loss of cellular adhesion to …
-
Natural history of sea-snake bite and poisoning: a clinical study
The toxaemia of snake-bite in man remains a challenge to the clinician. Fear of snakes and their bites is universal and colours the reaction of both patient and doctor. In each case the fear stems mainly from ignorance and a conditioned - contrary to popular belief, fear of snakes is not …
-
Methodological and biological studies of the amino acid oxidases.
… activity. The specificities of human, hog and snake venom amino acid oxidases were shown to be significantly different.
-
The identification and characterisation of antimicrobial peptides from snake and scorpion venom.
… action, and selectivity to prokaryotic membranes.Venoms are an under exploited source of AMPs, in this thesis AMPs have been identified, biologically characterised and their mechanism investigated. From snake venom two novel AMPs (6-7KDa) along with four potential phospholipase A2 (PLA2) proteins …
-
Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… CLEC-2 signalling in response to its ligand, the snake venom toxin rhodocytin, was also abrogated following tetraspanin over-expression. However, stable tetraspanin over-expression only partially reduced signalling. Moreover, knockdown of the major Jurkat cell tetraspanin, CD81, and deletion of …
-
The design and installation of an automatically controlled hydrocarbon fractionation unit
… glycohydrolase (NADase) from Bungarus fasciatus venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on the …
-
Purification and properties of Bungarus fasciatus venom NAD glycohydrolase
… glycohydrolase (NADase) from Bungarus fasciatus venom was purified over 1000-fold to electrophoretic homogeneity through a 3-step procedure which included affinity chromatography on Cibacron Blue agarose. The enzyme exhibited a broad pH profile with the optimum range between 7-8. Studies on the …
-
Studies on the Metabolism of Plant Cells in Tissue Culture
… deoxyribo- and ribonucleosides resulting from snake-venom digestion, contained no activity. Deoxyribonucleotides were inhibitory. 14. A ribonucleotide mixture prepared by alkaline hydrolysis of RNA isolated from rat liver and sheep liver, contained cytokinin activity. Deoxyribo- and …
-
Biophysical and Biochemical Properties of Salmonella Potsdam Bacteriophage P3/2
… <p>By means of deoxyribonuclease and snake venom phosphodiesterase enzyme degradation and mononucleotide separation by ion-exchange chromatrography, the following molar ratios for the P3/2<sup>vir </sup>DNA were determined: G-C 54.7-55.1 percent; A-T 44.9-45.3 percent.</p>
-
Toward a Quantitative Analysis of PARP-1 and Poly(ADP-ribosyl)ation in Cellular Senescence
… fractionation, boronate affinity chromatography, snake venom phosphodiesterase digestion, manipulation of PARP-1 reaction conditions, and immobilized metal affinity chromatography (IMAC) for the preparation of poly(ADP-ribosyl)ated protein samples prior to MS analysis using both MALDI-TOF and …
-
Entwicklung und Evaluation von IgY-Antikörpern gegen die Metalloproteinase ADAM12
… which are together with the SVMP’s (Snake Venom Metalloproteases) part of the reprolysin family of metalloproteases. ADAM12 is a part of the ADAM family. The two existing splice forms ADAM12-S and ADAM12-L are composed of a typical domain structure, which is a “signal peptide”, a …
-
Noxious Cellular Injury Triggers an Oxidative Stress Gene Response
… stimulated with <em>Crotalus atrox </em>(CA) venom. NGS data was assembled and annotated utilizing the Illumina paired-end RNA-seq approach with software packages HISAT2 and StringTie. Results showed 243 genes were differentially expressed (106 upregulated, 137 downregulated) between CA venom …