Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Smug1"”.
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DNA Damage and Cell Death Mediated by Thymidylate Synthase Inhibitors: Role of UNG and SMUG1 DNA Glycosylase
… monofunctional uracil-DNA glycosylase-1 (SMUG1), both of which have been implicated in the repair of U and 5-FU lesions. Interestingly, in spite of a decreased efficiency of UNG2 or SMUG1-depleted cells to remove 5-FU from a synthetic oligonucleotide, loss of UNG2 did not affect the …
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Methods to Probe the Function of Modified Bases in DNA
… 5-hmU enrichment sequencing is performed in SMUG1 knockdown HEK293T cells to determine the genomic location of 5-hmU in mammals. An increase in 5-hmU loci is observed upon SMUG1 knockdown. 5-hmU enriched regions are found to be T-rich and depleted in exons and promoters. Furthermore, 5-hmU …
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The Role of 5-Hydroxymethylcytosine in Genome Instability
… is validated by the observation that loss of the SMUG1 DNA glycosylase, which excises genomic 5hmU, rescues the hypersensitivity of BER-deficient Polb-/- cells to exogenous 5hmC. I also present preliminary data suggesting that endogenously generated 5hmC, produced via overexpression of the TET1 …
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UNRAVELING THE MOLECULAR MECHANISM OF BRCA2-POLTETA INTERPLAY IN PREVENTING SSDNA GAP ACCUMULATION DURING DNA REPLICATION
… ssDNA gaps and find that they mainly arise from SMUG1 processing of demethylation intermediates, such as 5hmC, leading to the generation of abasic (AP) sites. Additionally, we demonstrate that the accumulation of AP sites impedes fork progression, resulting in ssDNA formation through repriming …
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INVESTIGATING THE LINK BETWEEN HOMOLOGOUS RECOMBINATION PROTEIN BRCA2 AND DNA METHYLATION
… the cytosine methylation turnover proteins like SMUG1 partially contribute to the degradation of the nascent strands, while nucleotide excision repair proteins like DDB1 does not contribute. Taken together, our model suggests that the abasic site originating from cytosine methylation turnover …