Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"Smoothened"”.
-
Studies of Smoothened in Hedgehog Signaling Pathway
… transducer, the seven-transmembrane protein Smoothened (Smo) is highly regulated. It is still a mystery how Smo transduces graded Hh signal to downstream components. Although Smo shares some structural similarity with G protein coupled receptors (GPCR), there is little evidence that G …
-
Novel Insights into the Role of the Smoothened Cysteine Rich Domain in Hedgehog Signalling
… with numerous cancer and tumor conditions. The Smoothened (Smo) G protein coupled receptor (GPCR) functions as the signal transducer of the Hh pathway and is the most attractive drug target of the pathway. The structure of the Smo receptor includes seven membrane spanning domains, extracellular …
-
Chemically Based Interrogation of Smoothened, a Proto-Oncogenic Component of the Hedgehog Signal Transduction Pathway
… of the seven transmembrane oncoprotein, Smoothened (Smo). Thus, Smo is a high priority therapeutic target in Hh-associated cancers including basal cell carcinoma (BCC) and medulloblastoma. While a Smo antagonist is now approved for the management of BCC, therapeutic strategies focused on …
-
Machine learning and task disambiguation in hand-picked agriculture
… Piecewise quadratic interpolation was used on smoothened data to detect segments in trips taken by workers. Trip segments are then evaluated with the different profiles to find the best fitting profiles and the associated optimal parameters. Results indicated that the proposed framework …
-
The Role of Notch Signalling in Amplifying the Cellular Response to Sonic Hedgehog during Dorsal-Ventral Patterning of the Spinal Cord
… of key components of the Shh pathway, namely Smoothened and Patched1, to primary cilia. Activation of Notch increases ciliary localisation of Smoothened, whereas inhibition of Notch decreases exit of Patched1 from cilia upon Shh stimulation. Finally yet importantly, I find that high Notch …
-
Computational Approaches For Designing Protein/inhibitor Complexes And Membrane Protein Variants
… a water-soluble variant of the membrane protein Smoothened, wsSMO. This wsSMO stands to serve as a first-step towards developing membrane protein analogs of this important signaling protein and drug target.
-
Hedgehog - like Proteins are Involved in a Novel Glia - Neuron Signalling Pathway in C. elegans
… <em>bona fide</em> homologs of Hedgehog and Smoothened, does not possess the canonical Hedgehog signalling pathway that has been evolutionarily conserved across a wide range of organisms. However, our results suggest that a non-canonical Hedgehog-like signalling might in fact be present in …
-
MOLECULAR CHARACTERIZATION OF THE PUTATIVE NUCLEOTIDE- BINDING AND HYDROLYZING ACTIVITIES OF THE ELLIS VAN CREVELD (EVC) PROTEIN
… downstream processes after activation of the smoothened receptor (Smo), by the hedgehog proteins. Bioinformatic analysis revealed the presence of several structural motifs in EVC sequences including P-loop, leucine zipper, transmembrane domain, and nuclear localization signal. The putative …
-
Removal of Pax6 from cortical progenitors renders them abnormally likely to switch fate in response to Sonic Hedgehog activity
… of Shh agonist, developed for the protein Smoothened (SAG), which is a key part of the activation of Hedgehog signalling pathway. Results showed significant increases of Gsx2+, Dlx1+ Olig2+ cells in parallel with the increase of Shh agonist concentrations. We also investigated the …
-
The transcription factor Zfx is required for tumorigenesis caused by Hedgehog pathway activation
… These targets included the Hh signal transducer Smoothened (Smo). Smo expression data from Zfx-deficient BCC and MB cells in vivo and in vitro suggest that Zfx may directly regulate Hh pathway activation in some cancers. We identified two additional conserved downstream targets of Zfx, Dis3L and …
-
Ihh Signaling and Muscle Forces are Required for Enthesis Development
… throughout development using a conditional Smoothened (Smo) knockout mouse model specific to tendon lineage cells resulted in dramatic mineralization defects in the enthesis and reduced biomechanical behavior of the attachment.</p><p>Taken together, this thesis demonstrates that Ihh …
-
Bioinformatic Analysis of the Interaction Of EVC And EVC2 Proteins
… interaction between the ciliary protein EVC2 and Smoothened (Smo) at a specific ciliary compartment called the EVC zone. This complex is essential for proper Hh signal transmission and plays an important role in the molecular basis of EvC syndrome and Weyers Acrofacial Dysostosis. EVC and EVC2 are …
-
Establishment of hedgehog pathway overexpression in osteosarcoma cell lines leading to an anti-apoptotic genotype which can be inhibited by the common anti-fungal and hedgehog pathway inhibitor, itraconazole
… antifungal agent that has been found to inhibit Smoothened (SMO), a crucial G protein-coupled receptor located on the cell membrane and becomes activated on the primary cilium, which allows initiation of HH pathway signaling. We hypothesize that components of the HH pathway are overexpressed in …
-
Investigating the Role of Dispatched in Hedgehog Ligand Transport and Delivery
… the essential signal transducer of the pathway: Smoothened. Furthermore, mutations within Disp have been found in patients with Holoprosencephaly, the most common cause of human forebrain malformations, which is frequently caused by inhibition of Hh signaling during development. Very little is …
-
Activation mechanisms of non-class A G protein-coupled receptors
… activation mechanisms of Class F receptor Smoothened (SMO), six class B1 GPCRs - Glucagon receptor (GCGR), Glucagon-like Peptide 1 receptor (GLP1R), Parathyroid Hormone 1 receptor (PTH1R), Secretin Receptor (SCTR) and Pituitary Adenylate Cyclase 1 receptor (PAC1R), and fungal Class D …
-
Obstacles to women's participation in post-colonial education in Tanzania : what is to be done?
… thinker, and diligent woman penetrate falsely smoothened surfaces of unconventional and conventional institutions, in order to understand what each of them contribute to both enable and minimize women's participation in post-colonial education in Tanzania. Despite all the challenges that have …
-
Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
… cilia signaling partners, such as Patched1, Smoothened, and members of Gli family. Comparative morphometric analysis revealed that the mean length of reprogrammed cells was shorter than those of parental human fibroblasts. Morphometric analyses revealed that reprogramming resulted in an …
-
Understanding the Structure-property Relationships of Solid-state Additively Manufactured Stainless-steel Coatings
… had a greater frictional resistance due to the smoothened and strengthened surface. The corrosion rate was also decreased due to the densified and refined surface. In tribo-corrosion conditions, the repassivation kinetics of the passive film between sliding cycles became increasingly active, …
-
Comunicazione intercellulare e riprogrammazione del microambiente tumorale nella risposta patofisiologia del glioblastoma alla radioterapia
L’insorgenza e la progressione del glioblastoma (GBM) è supportata da un ampio range di fattori che influenzano lo stato metabolico, il setting genetico ed epigenetico del tumore e l’interazione con il microambiente circostante. Il complesso interplay tra cellule tumorali e microambiente innesca …
-
Astrocytic Sonic Hedgehog signalling in development and disease
Sonic hedgehog (Shh) signalling is a critical pathway in embryonic development, with known roles in cell differentiation, tissue patterning, and the establishment of body axes (Rowitch et al., 1999; Echelard et al., 1993; Ericson et al., 1997; Jessel and Lumsden, 1997). Beyond these canonical …