Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Smc5/6 complex"”.
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Gene expression from unintegrated HIV-1 is restricted by the SMC5/6 complex
… the host CRL4-DCAF1 cullin-RING ubiquitin ligase complex. To identify the responsible Vpr target, I performed a CRISPR-Cas9 forward genetic screen using a custom designed Vpr target sgRNA library. The screen identified SLF2, a poorly characterised protein which recruits the SMC5/6 complex to sites …
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Functional Analysis of the Human SMC5/6 Complex in Homologous Recombination and Telomere Maintenance
… recombination (HR). The SMC1/3 cohesin complex promotes HR by localizing to DSBs where it holds sister chromatids in close proximity to allow HR-induced strand invasion and exchange. The SMC5/6 complex is also required for DNA repair, but the mechanism by which it accomplishes this has …
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Revealing SMC5/6's Dynamic Behavior on Diverse DNA Topologies Through Single-Molecule Methods
… maintenance of chromosomes (SMC) protein complexes serve as critical molecular motors for maintaining genome stability and ensuring faithful genome propagation. As one of the three eukaryotic SMC complexes, the Smc5/6 complex maintains chromosomal stability by coordinating DNA repair …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… the intricacy associated with evolution of complex biological processes. This organization serves to exert spatiotemporal control of many DNA-transacting processes such as gene expression. Despite emerging progress, the biophysical mechanism underpinning eukaryotic genome organization …
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Investigating the role of NSMCE2 in cell cycle progression and genome maintenance
… the development and progression of cancer. The SMC5/6 complex is an evolutionarily conserved protein complex thought to be involved in this process, specifically its SUMO ligase NSMCE2 subunit. Prior to this thesis, CRISPR-Cas-9 mutant NSMCE2 cell lines were produced; NSMCE2 knockout, NSMCE2 …