Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"Small interfering RNA (siRNA)"”.
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To Develop a Small Interfering RNA (siRNA) Design and Information Resource to Facilitate Genetic Manipulaton of Human Cells
Part I: Small interfering RNAs (siRNAs) have revolutionized our ability to study the effects of altering the expression of single genes in mammalian (and other) cells through targeted knockdown of gene expression. In the past, there were a set of rules designed to develop siRNA which worked …
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Regulation of CYP3A5 in Pancreatic Ductal Adenocarcinoma
… the AsPC-1 PDAC cell line. This screen employed small interfering RNA (siRNA) targeting transcription factors and chromatin-associated proteins. We identified siRNA that decreased CYP3A5 mRNA and protein levels in AsPC-1 cells and probed into the potential mechanisms. Taken together, this study …
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Treatment of Ischemia Reperfusion Injury with RNA Interference
… were transfected with caspase-3, C3, and RelB small interfering RNA (siRNA) alone or in combination and then analyzed. The relative risk reduction of apoptosis in antimycin A treated cells with caspase-3 siRNA was 46.6% (p=0.019), RelB siRNA 42.8% (p=0.038), and complement 3 siRNA 13.9% …
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Strategic modification of oligonucleotides with carboxylic acids containing small molecules
Small interfering RNA (siRNA) are double-stranded RNA molecules used to manipulate gene regulation, by silencing gene expression. SiRNA molecules have been integrated into the therapeutic field, with several FDA-approved medications targeting liver-specific diseases. While effective, these …
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Synthesis and applications of oligonucleotide-bound alginate hydrogels
… spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA and RNA in both detection signal …
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Dual Assembly Nanoparticles Achieve Sustained Silencing of Znf304: A Novel Transcription Factor For Β1 Integrin
… and prevents anoikis. Targeting ZNF304 using small interfering RNA (siRNA) reduces cell survival, anoikis and migration in vitro. A novel dual assembly nanoparticle system (DANP) was designed for in vivo delivery and sustained gene silencing. DANP-ZNF304 siRNA led to sustained ZNF304 silencing …
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PI3K/Akt activation is critical for early hepatic regeneration after partial hepatectomy
… used the potent PI3K inhibitor, wortmannin, and small-interfering RNA (siRNA) targeting the p85ƒÑ and p110ƒÑ subunits to determine if total or selective PI3K inhibition would abrogate the proliferative response of the liver following resection. After partial hepatectomy in mice, there is an …
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The Effects of Atypical Protein Kinase C on TGFβ Signalling
… expression or activity using inhibitors and/or small interfering RNA (siRNA) prolongs the temporal phosphorylation of the downstream transcription factor Smad2 through altered receptor membrane trafficking. Furthermore, I showed that aPKC activity and expression alters the phosphorylation and …
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Identifying Novel Contributors to RNA Interference in Aedes aegypti
… yellow fever, dengue and chikungunya. The small interfering RNA (siRNA) pathway is a critical immune response for controlling viral replication in Aedes aegypti. The goal of this research is to identify components of the Aedes aegypti genome that influence this pathway. A transgenic …
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Role of the Sh3 and Cysteine-Rich Domain 3 (STAC3) Gene in Proliferation and Differentiation of Bovine Satellite Cells
… of adult cattle and transfected them with STAC3 small interfering RNA (siRNA) or scrambled siRNA. Cell proliferation assays revealed that STAC3 knockdown had no effect on the proliferation rate of bovine satellite cells. We assessed the differentiation status of bovine satellite cells by …
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CHARACTERIZATION OF FRONTAL CORTICAL CIRCGRIN2B_011731 IN OPIOID-INDUCED NEUROADAPATIONS
… In this dissertation we will examine circular RNA (circRNA) dysregulation following a rodent model of heroin self-administration and characterize a novel circRNA pathway regulating opioid induced neuroadaptations. We identified 76 circRNAs regulated by heroin self-administration, of these we …
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The Biogenesis of Small Interfering RNA in Neurospora Crassa
RNA interference is a well-conserved gene silencing mechanism in eukaryotes. It regulates various biological processes including development, genome defense and heterochromatin formation. RNAi is initiated by the production of dsRNA, which is processed by Dicer to produce small interfering RNA …
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The Role of Sphingosine Kinase 2 in Cell Growth and Apoptosis
… downregulation of endogenous SphK2 with small interfering RNA (siRNA) targeted to unique mRNA sequences decreased basal and doxorubicin-induced expression of p21 without affecting p53. In addition, downregulation of SphK2 decreased G2/M arrest in response to doxorubicin. Surprisingly …
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SMARCA4/BRG1-Inactivating Mutations as Potential Predictive Markers for Aurora Kinase A-Targeted Therapy in NSCLCs
… lesions create one of the largest subsets of Non-Small Cell Lung Cancers (NSCLCs). Since these mutations have been identified as bona fide tumor suppressors, efforts have focused on understanding the pathology of cancer caused by SMARCA4/BRG1 aberrations. However, no therapeutic agent has been …
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The development of novel strategies to modulate epithelial sodium channel (ENaC) activity in chronic obstructive pulmonary disease (COPD)
… drug delivery system to transport an αENaC small interfering RNA (siRNA) cargo into primary bronchial epithelial cells (PBECs). RALA/siRNA nanoparticles were well-tolerated by submerged PBECs and transfection of cells led to a high level of αENaC mRNA knockdown. Whilst local delivery to PBEC …
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Role of Macrophage Apoptosis in Atherosclerosis.
… in macrophage-like P388D1 cells by using small interfering RNA (siRNA) to suppress Bax expression, as well as in peritoneal macrophages isolated from Bax null mice (Bax-/-). Apoptotic activities in both cell types deficient for Bax were significantly reduced compared to that in control …
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Characterisation of Androgen Receptor Signalling and Metabolism in Prostate Cancer
… the AR) and Hox Transcript Antisense Intergenic RNA (HOTAIR) (a long noncoding RNA) can interact with the AR and are found to be elevated in CRPC. To investigate their effect on AR activity, luciferase reporter assays were performed. Results demonstrated that both factors can repress AR activity. …
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Investigating Hybrid Cyclic/Linear and Linear Peptides as Vehicles for Nucleic Acid Delivery
<p>Small interfering RNA (siRNA) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated Protein 9 (Cas9) are advanced biotechnological approaches that have been able to temporarily downregulate gene expression through post-transcription RNA interference or …
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Development and characterization of chemical modifications for siRNA strand selection
Small interfering RNA (siRNA) are non-coding, double-stranded RNA that enable efficient gene silencing through RNA interference (RNAi) mechanisms. The primary effector of RNAi is an RNA-guided nuclease, Ago2, which selects one of the two strands as a guide to form the RNA induced silencing complex …
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The Influence of SPARC on Collagen Deposition in Asbestos-Induced Pulmonary Fibrosis
… these findings, SPARC expression was reduced by small interfering RNA (siRNA) in wild-type mice already suffering from fibrosis. Collagen deposition in the fibrotic mice that received the SPARC siRNA vector showed a significant decrease in collagen accumulation when compared to those that did not …
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