Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Small Ubiquitin-like MOdifier (SUMO)"”.
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Characterisation of Small Ubiquitin-like Modifier (SUMO) protein complex expression in human cancer cells to identify novel therapeutic targets
SUMOylation is a post-translational modification where a number of small ubiquitin-like modifier proteins (SUMO) are conjugated to lysine residues of the target proteins and contribute to various cellular functions essential for cancer cell survival and proliferation. The unique behaviours, …
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Elucidating the Role of RanBP2/Nup358 in the Regulation of Cytokines mRNAs
RanBP2 is a small ubiquitin-like modifier (SUMO) E3-ligase located at the cytoplasmic filaments of the nuclear pore complex, and is also present at low levels in the cytosol. Mutations in RanBP2 are linked to acute necrotizing encephalopathy 1 (ANE1), a rare pediatric disorder marked by excessive …
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
Small ubiquitin-like modifier, SUMO, is a member of the ubiquitin-like protein family. Similar to ubiquitin, SUMO is conjugated to target proteins as a post-translational modification via an E1, E2, and E3 enzymatic cascade, in a process known as SUMOylation. SUMOylation leads to altered target …
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Developmentally regulated SUMOylation in the ciliate Tetrahymena thermophila
<p>The Small Ubiquitin-like MOdifier (SUMO) protein regulates numerous nuclear events such as transcription, mitosis and meiosis and DNA repair. These processes are critical to the programmed nuclear events of conjugation in ciliates and provide the potential to investigate developmentally …
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Interplay between collapsin response mediator protein 2 (CRMP2) phosphorylation and sumoylation modulates NaV1.7 trafficking
… collapsin response mediator protein 2 (CRMP2) by small ubiquitin-like modifier (SUMO) and phosphorylation could affect Nav trafficking and function. Expression of a CRMP2 SUMOylation incompetent mutant (CRMP2-K374A) in neuronal model CAD cells, which express predominantly Nav1.7 currents, led to a …
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The identification and characterisation of proteins interacting with SUMO-ubiquitin hybrid chains
… modification of substrate proteins by either ubiquitin or the small ubiquitin-like modifier, SUMO. In recent years, points of convergence between the once thought distinct and independent SUMO- and ubiquitin-conjugation pathways have been identified. The ubiquitin E3 ligase RNF4 catalyses the …
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Functional Analysis of the Human SMC5/6 Complex in Homologous Recombination and Telomere Maintenance
… these NSE proteins, MMS21/NSE2, is a ligase for small ubiquitin-like modifier (SUMO). Depletion of MMS21 by RNA interference (RNAi) sensitizes cells toward DNA damageinduced apoptosis. This hypersensitization of MMS21-RNAi cells is not due to a defect in DNA damage-induced cell cycle checkpoint, …
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Protein SUMOylation is a Sex-Specific Regulator of Fear Memory Formation in the Amygdala
SUMOylation is a type of post-translational protein modification similar to ubiquitination and it involves the covalent attachment of a small ubiquitin-like modifier (SUMO) protein to the lysine residue of a target substrate. While there is strong evidence for the role of protein ubiquitination in …
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Regulation of the oncogenic PIM1 kinase by SUMOylation
… active site of PIM1 that is recognised by the Small ubiquitin-like modifier (SUMO). By site-directed mutagenesis of individual lysine residues in PIM1, I showed that PIM1 is covalently modified by SUMO at he consensus K169 site, and also at a second promiscuous site. However, the K169R mutant …
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Defining the mechanism of arsenic-induced degradation of PML
… translational modification of PML and with the small ubiquitin like modifier (SUMO). SUMO modification of PML recruits the SUMO targeted ubiquitin E3 ligase RNF4 via four SUMO interaction motifs within the N-terminal region of RNF4. PML is then ubiquitylated and targeted for proteasomal …
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NEUROPROTECTIVE THERAPIES CENTRED ON POST-TRANSLATIONAL MODIFICATIONS BY SUMOYLATION
… driver of the pertinent pathobiology), it will likely not be possible to identify a single dominant therapeutic target. Therefore, a focus on i) plurifunctional molecular pathways and ii) plurifunctional therapeutic modalities capable of influencing brain ischemia are urgently warranted. A …
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Applications of Ion Mobility Mass Spectrometry - Screening for SUMOylation and Other Post-Translational Modifications
… to substrate proteins. Conjugation to the Small Ubiquitin-like MOdifier (SUMO) is a highly conserved and regulated posttranslational modification that is often restricted to highly specific cellular events and a number of obligatory nuclear regulatory processes. Although much is known about …
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Amyloid-beta signaling in physiology and pathology: from astrocytes to SUMO
… that post-translational modification with the small ubiquitin-like modifier (SUMO) is required for normal synaptic and cognitive function but can be impaired by Abeta oligomers. I discovered that SUMOylation was significantly reduced in brain tissue from AD patients and a transgenic mouse model …
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A functional analysis of the epigenetic regulator CTCF
… Finally, with this system we found and analyzed SUMO-motif sites in the NT- and CT-regions. Our data suggests that sumoylation of the NTAD reduces its transactivation and chromatin opening activities, while sumoylation of the CT region demonstrated enhancer blocking activity. This is the first …