Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Small RNA sequencing"”.
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Quality checking and expression analysis of high-throughput small RNA sequencing data
The advent of high-throughput RNA sequencing (RNA-seq) methods have made it possible to sequence transcriptomes for the cell-wide identi�cation of small non-coding RNAs (sRNAs) and to assess their regulation using di�erential expression analysis by comparing two or more di�erent conditions. During …
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Small RNAs in Tomato: from defence to development
RNA silencing is a major regulator of gene expression in plants, controlling from development to transposable element silencing and stress responses. As part of the silencing machinery, micro (mi)RNAs orchestrate silencing of their targets, either directly or through cascades of secondary small …
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Investigating the role of RNA 5- methylcytosine on ribosomal and transfer RNAs in Arabidopsis thaliana
RNA 5-methylcytosine (m5C) is implicated to have multiple biological and molecular roles in single cell and multicellular organisms, including regulation of mRNA translation and plant growth. However, there are still many questions waiting to be answered. In my research, I addressed the roles of …
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Interactions of Mammalian Retroviruses with Cellular MicroRNA Biogenesis and Effector Pathways
<p>The cellular microRNA (miRNA) pathway has emerged as an important regulator of host-virus interactions. While miRNAs of viral and cellular origin have been demonstrated to modulate viral gene expression and host immune responses, reports detailing these activities in the context of mammalian …
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Mechanisms and impact of alternative transposition-induced segmental duplications
… PCR, Southern blotting, qRT-PCR, microarray, mRNA-sequencing, small RNA-sequencing, and a self-developed program (STRAND: Search for Transposon-Induced Tandem Direct Duplications) to study these questions. We discovered new genome rearrangement mechanisms, including transposition of paired DNA …
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Extracellular Vesicles as Mediators of Communication Between Endothelial Cells and Fibroblasts
… transferring regulatory molecules such as microRNAs (miRNAs) to nearby or distant cells. This study investigated whether EVs secreted by human dermal microvascular endothelial cells (EC-EVs) influence fibroblast behavior. We isolated and characterized EC-EVs and confirmed their uptake by primary …
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Endogenous short interfering RNAs that silence chalcone synthase result in pigment pattern formation on soybean seed coats
… (CHS) genes leading to production of CHS siRNAs which in turn degrade CHS mRNAs resulting in yellow seed coats (Tuteja et al., 2009 and Vodkin et al., 2012). However, it was not known if the same phenomenon applied to the yellow and pigmented regions within the same seed coats that have …
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EVOLUTIONARY AND MECHANISTIC INTERACTIONS IN VIRUS TRANSMISSION BY APHIDS
… in virus transmission phenotype. Finally, using small RNA sequencing, I showed that aphids do not activate their small interference RNA (siRNA) antiviral defense against PLRV, which provides additional evidence for the lack of replication of Luteovirids in their aphid vectors. However, aphids …
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Development of Computational Tools and Resources for Cotton microRNA Analysis
MicroRNAs (miRNAs) are an extensive class of small regulatory RNAs which regulate gene expression at the posttranscriptional levels. miRNAs target genes for mRNA cleavage or translation inhibition based on the complementary between the mRNAs and its corresponding miRNAs, and these miRNA target …
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Molecular characterisation of the role of systemic infections in Alzheimer’s disease brain
… samples from the UK Brain Bank Network, bulk RNA sequencing revealed that systemic infection elicits distinct molecular responses in AD compared with controls. Differential expression and co-expression network analyses identified infection-dependent alterations in immune, vascular, and …
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
… are the core components of the mammalian microRNA-induced silencing complex (miRISC). This machinery mediates post-transcriptional silencing of mRNA targets by sequence-specific hybridization with microRNAs (miRNAs) in the cytoplasm. Localization to subcellular compartments is critical to …
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A mosquito-specific bZIP transcription factor and the influence of a Y-specific gene on sex determination in Anopheles stephensi
… transcription factor and its intronic miRNA cluster and characterize a novel Y chromosome gene in An. stephensi. The aims of these experiments were to expand on the knowledge of genes involved in embryonic development and sex determination with potential application in vector control …
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Insights into the piRNA pathway through 16 strains of mice
… several classes of non-coding regulatory RNAs. One such class of small RNAs - PIWI-interacting RNA (piRNAs) - are exclusively expressed in the germlines of mammals. This thesis provides an exhaustive bioinformatic exploration of piRNA biogenesis and dynamics utilising a large …
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Mechanisms Underlying Mesenchymal Stem Cell-Derived Extracellular Vesicle Modulation of Sensory Neuron Excitability
… These findings suggest that EV internalisation is at least partially required and that transcriptional changes may underlie the observed effects. Consistent with this hypothesis, inhibition of transcription with actinomycin D attenuated the full protective effect of MSC-EVs. A review …
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NEW FINDINGS INTO TARGET-DIRECTED MICRORNA DEGRADATION MECHANISM
MiRNAs are a class of small non-coding RNAs that function in post-transcriptional gene silencing by interacting with target RNAs. A novel mechanism in control of miRNA levels, called Target-Directed miRNA degradation (TDMD), has been described, which involves specific transcripts able to interact …
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Investigating the Role of the IP₃ Signalling Pathway in RNA Interference in C. elegans
… (IP₃) mediated signalling and RNA interference are widespread processes with fundamental roles in animal cell function. In the nematode C. elegans these two pathways have been shown to intersect such that IP₃ signalling mutants display an altered exogenous RNAi response. IP₃ is …
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Characterization of the endothelial cells response to trophoblast debris
… extrudes extracellular vesicles into the maternal circulation. This deportation of placental extracellular material creates an extensive feto-maternal interface dispersed around the maternal body and exposes various maternal cells to a large amount of fetal/placental material. Preeclampsia, a …
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PART I CRYSTAL STRUCTURE OF A DIMERIZATION DOMAIN OF DROSOPHILA CAPRIN. PART II CHARACTERIZATION OF TWO CAS13B CRISPR-CAS SYSTEMS FROM PORPHYROMONAS GINGIVALIS
… with Caprin protein family members, which are RNA binding proteins that play critical roles in many important biological processes, such as synaptic plasticity, stress response, innate immune response and cellular proliferation. One of the Caprin protein family members, Caprin-1, is involved in …
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Multi-omics approaches for diagnosis, prognosis and response to treatment of colorectal cancer.
… profiling and highly sensitive next-generation sequencing analyses to differentiate the subtypes of CRC into prognostic groups can also lead to a better understanding of adequate CRC treatment, improving prognosis and patients’ quality of life. The aim of this study, conducted in collaboration …
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Molecular Characterization of Viruses Infecting Greenhouse Vegetables in Ontario
… vegetables in Ontario, we conducted in-depth sequencing of small RNAs isolated from virally infected plants used next-generation sequencing technology. Subsequent bioinformatics analyses revealed six viral pathogens including Bell pepper endornavirus (BPEV), Parietaria mottle virus (PMoV), …
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