Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"SleB"”.
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Regulation of the Spore Cortex Lytic Enzyme SleB in Bacillus anthracis
… work investigated the regulation of the GSLE SleB by other proteins in the spore. A full understanding of how GSLEs are held inactive in the dormant spore and are activated during germination could lead to development of simplified spore decontamination strategies in which spore germination is …
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The Roles of the Germination-Specific Lytic Enzymes CwlJ1, CwlJ2, and SleB in Bacillus anthracis Spores
… In this work three GSLEs, CwlJ1, CwlJ2, and SleB are identified in B. anthracis and are investigated in order to better understand their functions. Genetic manipulation of cwlJ1, cwlJ2, and sleB was fundamental to this work. First, reporter gene fusions revealed that all three are expressed …
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Relative Expression of Germination Genes SleB, GerAC, and Gpr in Heat Treated Bacillus licheniformis and Bacillus subtilis
… specific for GerAC, <em>gpr</em>, and <em>SleB</em> genes to evaluate their relative expression and potential for indicators of lag phase in germination. Gene expression studies with qPCR were carried out during germination in <em>Bacillus subtilis </em>and <em>Bacillus licheniformis</em> …
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Structural and Functional Studies of Sensor Kinase RetS from Pseudomonas aeruginosa and Peptidoglycan Hydrolase SleB from Bacillus anthracis
… the germination-specific lytic transglycosylase SleB from Bacillus anthracis displays a unique active site topology-<br />germination-specific lytic enzymes (GSLEs) that degrade the unique cortex peptidoglycan to permit resumption of metabolic activity and outgrowth. We report the first crystal …
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Molecular analyses of YhcN/YlaJ and spore-associated proteins involved in germination of Bacillus cereus spores
… appears to influence the precise location of SleB within spores and or possibly influence the properties of the inner membrane such that initiation of germination is extended considerably in DyhcN strains. Work associated with the early stages of germination revealed that Bacillus cereus …
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Swept Line Electron Beam Annealing of Ion-Implanted Semiconductors
The capabilities of a Swept Line Electron Beam (SLEB) in annealing ion-implanted semiconductors are examined. This technique employs a fixed geometry, line-shaped electron beam through which implanted samples are mechanically scanned. In general, this technique can produce annealing results …
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Structural and functional insights to Bacillus spore germination and toxin engineering for sustainable agriculture
… (GRs), the SpoVA CaDPA channel, and the SleB cortex-lytic enzyme, which are all major components of the germination apparatus. The presented data indicate that the GerI GR can function independently of the GerQ GR in triggering a germination response to inosine in B. cereus spores, …
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Characterization of the Bacillus anthracis SleL Protein and its Role in Spore Germination
… to cortex lytic enzymes CwlJ1, CwlJ2, and SleB. The loss of sleL results in a delay in the loss of optical density during germination. However, spores are capable of completing germination as long as CwlJ1 or SleB remains active. HPLC analysis of muropeptides collected from B. anthracis …
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Investigating proteins that influence membrane-associated germination processes in Bacillus subtilis spores
… the absence of the spore lytic enzyme SleB seems to alter the crosslinking pattern of the lipoproteins, suggesting either it's interacting or helping facilitate lipoprotein interactions. Fluorescence microscopy reveals an unequal spatial distribution of the lipoproteins on the spore …
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Characterization of Bacillus Spore Membrane Proteomes and Investigation of Their Roles in the Spore Germination Process
… is not clear. We also demonstrated that YpeB and SleB, in the absence of their partner protein, were degraded by unknown proteases other than HtrC during spore formation. Identification and characterization of these unknown proteases would be a future direction for revealing the roles of proteases …