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Showing 1 to 14 of 14 for “"Skp2"”.
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Developing Constrained p27 Peptides to Target the Oncogenic E3 Ubiquitin Ligase SCF-Skp2
… enzyme, and E3 - ubiquitin ligase. The SCF-Skp2 complex is one of 600 E3s in the human genome, and Skp2 serves as a substrate recognition subunit. Skp2 is an oncoprotein that exerts its oncogenic functions through degradation of specific substrates. A major target of SCF-Skp2 is the …
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Discovery and Effects of Pharmacological Inhibition of The E3 Ligase Skp2 By Small Molecule Protein-Protein Interaction Disruptors
<p>Skp2 (S-phase kinase-associated protein 2), one component of the SCF E3 ubiquitin ligase complex, directly interacts with Skp1 and indirectly associates with Cullin1 and Rbx1 to bridge the E2 conjugating enzyme with its protein substrate to execute its E3 ligase activity. Skp2 is an Fbox protein …
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Novel Posttranslational Modification In Lkb1 Activation and Function
… by the Skp1-Cul1-F-box-protein/Skp2 (Skp2-SCF) ubiquitin ligase complex is critical for LKB1 activation by a mechanism of maintaining the LKB1-STRAD-MO25 complex integrity. We further demonstrate that oncogenic Ras acts upstream of Skp2 to promote LKB1 polyubiquitination by …
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Cross-modulatory Actions of Cell Cycle Machinery on Estrogen Receptor-alpha Level and Transcriptional Activity in Breast Cancer Cells
… and major regulator of cell cycle progression, Skp2, in the proteasomal degradation of ERα upon activation by p38MAPK. Interestingly, this regulation of ERα by sequential actions of p38MAPK and Skp2 that was observed specifically during the G1/S transition and S-phases of the cells cycle was …
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The Role of Amp-Activated Protein Kinase (Ampk) In Tumorigenesis
… discovered that AMPK directly phosphorylates Skp2 at S256 residue and this phosphorylation is essential for Skp2 SCF complex integrity and E3 ligase activity to ubiquitinate Akt, which is critical for Akt activation. Also, Skp2 S256 phosphorylation can be induced by many stress and EGF. The …
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Μελέτη ρυθμιστών του κυτταρικού κύκλου και παραγόντων που εμπλέκονται στη διεργασία αποδόμησης των P21cip1 και P27kip1 σε λεμφώματα Β-κυτταρικής αρχής. Συσχέτιση με κλινικές παραμέτρους
… είναι το μονοπάτι μέσω του συμπλόκου SCF (Skp2-cul1-Skp1) Ε3 λιγάσης της ουβικουιτίνης. Αρχικά οι πρωτεΐνες p27 και p21 φωσφορυλιώνονται από το σύμπλκο κυκλίνης Ε/Α-CDK2 στη θρεονίνη 187 (Thr187) και στη σερίνη 130 (Ser130) αντίστοιχα, και εν συνεχεία αναγνωρίζονται από το σύμπλοκο SCFSkp2, …
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The role of conexin 43 in murine sertoli cell development
… cell cycle proteins namely, p27Kip1, p21Cip1 and SKP2 (also known as CDKN1B, CDKN1A and p45, respectively). Roles of these cell cycle regulators on Sertoli cell development have been studied and Sertoli cell numbers were significantly altered in the absence of one or more of those cell cycle …
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Characterisation of the effects and mechanism of action of rapamycin and genistein on acute myeloid leukemia using high-throughput techniques
… patients. Our study showed that rapa represses Skp2, an important constituent of the protein ubiquitination pathway. Working on this clue, we identified that the time dependent G1 arrest is in fact the result of the inhibition of Skp2, leading to the accumulation of p27, which in turn causes …
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The role of p27 phosphorylation in mediating atRA sensitivity of ovarian carcinoma cells
… was accompanied by a decrease in the levels of skp2 protein, protein that plays a critical role in the degradation of p27. Similar results were not observed in SKOV3 cells which are not growth inhibited by atRA treatment. Finally, we demonstrated that overexpression of a mutant of p27 that …
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Identification and functional characterisation of novel APC/C interacting proteins
… securin and S-phase kinase-associated protein 2 (SKP2) for proteasomal-dependent degradation. APC/C substrates and coactivators are aberrantly expressed in many cancers. It is thought that the APC/C can also regulate cellular proliferation by controlling p21 and cell division cycle 6 homologue …
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Targeting the N-myc oncoprotein using nanobody technology
… proteins bind, such as the E3 ubiquitin ligase Skp2. Nanobodies against the transactivation domain were more specific for N-myc and were shown to inhibit its Skp-2-mediated ubiquitylation. This could provide novel means of eradicating tumours based on a study showing that inhibition of …
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Effects of carbohydrates on iron metabolism in intestinal and liver cells
… Cyclin D1 and Cyclin D2, and the proto-oncogene Skp2; decreased mRNA expression of HNF4A, a key liver transcription factor, but with increased HNF4A protein expression; increased HepG2 mRNA and protein expression of the “cancer chaperone” Heat Shock Protein 90; and significantly decreased mRNA …
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MECHANISMS OF SUBSTRATE DEGRADATION AND RECRUITMENT BY CULLIN E3 LIGASES
Protein degradation plays an important role in regulating many cellular processes. Ubiquitination, a prerequisite for protein degradation, is mediated by the Cullin E3 ubiquitin ligase family. The aim of this study is to achieve a better understanding of the function and regulation of Cullin E3 …