Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Site-specific integration"”.
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Investigating "difficult-to-express" monoclonal antibody frameworks in transient and site-specific integration-based Chinese hamster ovary cell expression systems
… expression, we have leveraged both transient and site-specific integration-based (SSI) expression platforms to study DTE mAb variants at higher throughput in industrially relevant mAb formats, cell lines, and culture conditions. The rational selection and transient expression of 178 single amino …
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Adeno-associated virus type 2 as vector for human gene therapy: Characterization of virus-host interactions
… of AAV in human gene therapy: First, the loss of site-specific integration of recombinant AAV vectors (rAAV) due to the deletion of the rep gene, and secondly, the potential neutralization of AAV gene therapy vectors by preexisting antibodies. It could be demonstrated that site-specific …
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Adeno-associated virus rep78 expression in Arabidopsis thaliana
… (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current …
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Genetic linkage of multiple modifications in the pig
… of target genomes. Modifications can be site-specific or random. Though inefficient, homologous recombination allows for precise genome modification. Random integration can be very efficient, but lacks the opportunity to control the orientation and location of the introduced DNA. Thus, an …
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Mutational Analysis of NBU1 Attachment Sites Reveals a Novel Recombination Mechanism
… related conjugative transposons. NBU1 integrates site specifically into the Bacteroides thetaiotaomicron chromosome primary target site, attBT1-1. NBU1 integrase (IntN1) catalyzes recombination of the NBU1 attachment site, attN1, and the attBT1-1 site. IntN1 is a member of the lambda family of …
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… plasmid DNA as a vector for transfection and integration of DNA into the genome. Chemical-mediated procedures were used to deliver plasmid DNA to the mouse mammary epithelial cell line, HC11, and to primary bovine mammary cells in vitro. Four delivery vehicles were used; calcium phosphate …
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Consequences of a mobile genetic element integrated at secondary locations
… including antibiotic resistance. Many ICEs are site-specific in that they integrate preferentially into a primary attachment site in the bacterial chromosome. Site-specific ICEs can integrate into secondary locations, but little is known about the consequences of integration. Using ICEBs1, a …
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
… the first part of our project, we established a site-specific integration system with enforced local transcriptional repression in colorectal cancer cells and monitored the occurrence of de novo DNA methylation in exogenous fragments containing a CGI-promoter and repetitive elements. Initial de …
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Expression Of Hepatitis C Viral Non-structural 3 Antigen In Transgenic Chloroplasts
… trnI & trnA homologous flanking sequences for site specific integration into the chloroplast genome. Chloroplast integration of the NS3 gene was first confirmed by PCR. Southern blot analysis further confirmed site-specific gene integration and homoplasmy. The NS3 protein was detected in …
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Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts
… fusion protein separated by a furin cleavage site was expressed via the tobacco chloroplast genome and used as a visible marker. Site specific integration of the transgene was confirmed by PCR analysis. Southern blot analysis confirmed homoplasmy. Immunoblot analysis confirmed the expression …
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… the insertional oncogenesis caused by random integration into the genome, we postulated that targeted insertion of the gene of interest through homologous recombination would prove to be a safer alternative to random viral insertion of a gene. To this end, we developed several pairs of TAL …
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Rationale Entwicklung rekombinanter CHO-Zellen basierend auf Histonanalyse und RNA-Seq: Identifizierung und Verwendung stabiler Integrationsorte
… for biopharmaceutical protein production. Random integration of a transgene into the genome during cell line development often leads to a loss of productivity during upstream processing. This is due to loss of gene copies or epigenetic silencing of the recombinant promoters. Both effects depend on …