Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Site-directed spin labeling"”.
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A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
… is known about the details of these binding sites, and it was not clear to what extent SecA interacted with these diverse ligands at distinct, adjacent or overlapping surfaces along its extended structure. We investigated these issues using electron paramagnetic resonance (EPR) spectroscopy …
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Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
… structure using the reporter group method of site-directed spin labeling (SDSL), along with electron paramagnetic resonance (EPR) spectroscopy. In this work, B and C parameter calculations from EPR line-shape theory express receptor differences quantitatively as a spectrum of conformational …
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Structure and Dynamics of AcrA, a Periplasmic Component of a Multidrug Efflux Pump
… the role of AcrA in the efflux mechanism, site-directed spin labeling (SDSL)/EPR (electron paramagnetic resonance) spectroscopy is used to investigate the structure and dynamics of AcrA in solution. My results demonstrated that AcrA is a dynamic protein that undergoes pH-dependent and …
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Informing Therapeutic Approaches For Infectious Disease And Heart Disease, Focusing On Cell-Free Synthesis Of Bacteriophages And Calcium Transport By Serca
… my work in the Thomas Lab, where I utilized site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) to investigate the structural model by which SERCA, PLB, and DWORF regulate intracellular calcium transport in cardiac muscle. I detail the methodology behind purifying and …
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Structural Polymorphism in Tau Filaments: An Implication for Neurodegenerative Diseases
… the other half has four repeats (4R tau).</p> <p>Site-directed spin labeling of recombinant tau in conjunction with electron paramagnetic resonance spectroscopy was used to obtain structural insights into tau filaments. The studies showed that the filaments of 4R tau and 3R tau share a highly …