Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Single-stranded DNA binding protein"”.
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… referred to as leishmaniasis. The major surface protein on all species of Leishmania is a highly abundant 63 kDa glycoprotein referred to as GP63. GP63 has been characterized as a cell surface protease, however, its exact role in the Leishmania life cycle is not clear. The genes encoding GP63 are …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
Single-stranded DNA binding (SSB) proteins are essential accessory proteins that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
Understanding how proteins work together to perform vital cellular functions, such as replicating and repairing DNA, not only extends our understanding of fundamental biology, but can also lead to important medical interventions. As a necessary first step to understanding larger systems, we focus …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
DNA inside a cell is continuously damaged through multiple mechanisms including environmental exposure to radiation, chemical agents, or UV light. Certain products of the cell's own metabolism, such as reactive oxygen species, can also damage the DNA. In the worst-case scenario, this damage results …
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1. X-ray crystallographic analyses of the gene V protein from Ff bacteriophage, a single-stranded DNA-binding protein, and its Y41 mutants 2. X-ray crystallographic analyses of several anticancer drugs and their interactions with DNA
In biological systems, DNA/RNA-protein interactions as well as drug-DNA interactions play important roles in genetic processes. A fundamental understanding of these processes requires a detailed structural knowledge of DNAs, proteins, drugs, as well as their complexes with each other. Gene V …
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X-ray diffraction analysis of gene V protein encoded by filamentous bacteriophage Ff
The gene V protein encoded by bacteriophage Ff is a single-stranded DNA binding protein and it plays an important role in the replication cycle of the phage. The crystal structure of the gene V protein was determined using multiwavelength anomalous diffraction on the selenomethionine-containing …
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Mechanisms of Palindrome-Mediated Chromosomal Instability
Palindromes are highly unstable DNA sequences that induce chromosomal rearrangements and underlie cancer and hereditary diseases such as Emanuel Syndrome. Despite being a significant source of genomic instability, the mechanisms governing palindrome fragility in eukaryotic cells are not fully …
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Cloning and Characterization of Replication Protein A from Dictyostelium discoideum
The gene encoding the Dictyostelium replication protein A large subunit (DdRPA1) has been cloned by screening of an EcoR I partial genomic library and a Hind III genomic sub-library. The complete nucleotide sequence, including the promoter region of the gene has been obtained by sequencing. Though …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
DNA damage is ubiquitous to all organisms and very complex pathways have evolved to recognize and repair these lesions. The most deleterious DNA damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. In human cells, there exist two canonical pathways of DSB …
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Quantitative all-atom and coarse-grained molecular dynamics simulation studies of DNA
… genetic information for all life on earth—DNA—is a polymer with unusual physical properties. The mechanical and electrostatic properties of DNA are utilized extensively by cells in the replication, regular maintenance, and expression of their genetic material. This can be illustrated by …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
Recent advances in single-molecule fluorescence imaging techniques have allowed the direct observation of protein dynamics on DNA, but the progress has been largely limited to double-stranded DNA (dsDNA) or short single-stranded DNA (ssDNA). Here, a single molecule imaging approach for observing …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
"One of the critical proteins involved in DNA replication across the three domains of life is single-stranded DNA-binding protein. At the replication fork, these proteins bind to stabilize and protect transiently exposed ssDNA from nucleases during DNA transactions. The ssDNA-binding protein is …
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Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage
<p>Human replication protein A (RPA) is an eukaryotic single-stranded DNA binding protein directly involved in a variety of DNA metabolic pathways including replication, recombination, DNA damage checkpoints and signaling, as well as all DNA repair pathways. This project presents 2 novel roles of …
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Co-factors of LIM-HD transcription factors in neural development and axon pathfinding in zebrafish
… transcription factor signaling. LIM homeodomain proteins (LIM-HDs) are an important class of transcriptional regulators and involved in neural development as well as neuron fate decision in vertebrates. DD domain dimerization of CLIM (cofactor of LIM-HDs) can activate LIM-HDs and downstream gene …
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Single-molecule optical trapping studies of the regulation mechanisms of XPD helicase
DNA is more than just a molecule. It is the blueprint of life, encoding the instructions that connect generations, shape identities, and reveal the mysteries of biology. Maintaining DNA integrity is essential, as repair, replication, and protective networks ensure that the genetic code is …