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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 171 for “"Single-stranded DNA"”.
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Transforming Activity in Single-Stranded Dna From Bacillus Subtilis
Made available in DSpace on 2014-12-09T17:35:44Z (GMT). No. of bitstreams: 1 6711832.pdf: 3258275 bytes, checksum: df05fff3da0ff175a81f77aff9db44d9 (MD5) Previous issue date: 1967
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Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution
<p>SINGLE-STRANDED DNA SCANNING AND DEAMINATION WITH SINGLE-MOLECULE RESOLUTION</p> <p>by</p> <p>GAYAN SENAVIRATHNE</p> <p>December 2013</p> <p>Advisor: Dr. David Rueda & Dr.Ashok Bhagwat</p> <p>Major: Chemistry (Analytical) </p> <p>Degree: Doctor of Philosophy</p> <p>APOBEC3G (Apo3G) and …
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Graphitic Surface Attachment by Single-Stranded DNA and Metal Nanoparticles
… to be explored. The noncovalent binding of single-stranded DNA oligonucleotides to carbon nanotubes and graphene has been seen to give rise to effective gas sensors. We examine similar systems to each of these in turn, imaging carbon nanotubes decorated with single-stranded DNA in …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
Recent advances in single-molecule fluorescence imaging techniques have allowed the direct observation of protein dynamics on DNA, but the progress has been largely limited to double-stranded DNA (dsDNA) or short single-stranded DNA (ssDNA). Here, a single molecule imaging approach for observing …
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Comparative Immunological Analyses of Anti-Deoxynucleotide and Anti Single-Stranded Dna Antibodies
Made available in DSpace on 2014-12-14T07:14:48Z (GMT). No. of bitstreams: 1 8004137.pdf: 6213863 bytes, checksum: d5b92c3339cbb096a98d432d17a51f9f (MD5) Previous issue date: 1979
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Single stranded DNA: A new model system for single molecule polymer dynamics
… nearly two decades, fluorescently-labeled double stranded DNA (dsDNA) has been the model system for studying single molecule polymer dynamics in non-equilibrium conditions. However, dsDNA is a semiflexible polymer with markedly different local molecular properties compared to flexible polymer …
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Cloning and characterization of a single-stranded DNA-binding protein of Leishmania major
… of direct head to tail tandem repeats and single dispersed gene copies. In the present study, a single repeat unit of the Leishmania donovani GP63 tandem array was cloned and sequenced. Alignment of the L. donovani GP63 gene sequence with the previously determined GP63 gene sequences from …
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
DNA replication is a complex process involving many proteins that must be performed for a cell to proliferate. In this work, we will study three members of the replication system: single-stranded DNA, single-stranded DNA binding proteins, and the sliding clamp and clamp loader proteins. …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
Single-stranded DNA binding (SSB) proteins are essential accessory proteins that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
… functions, such as replicating and repairing DNA, not only extends our understanding of fundamental biology, but can also lead to important medical interventions. As a necessary first step to understanding larger systems, we focus on a two-protein system involved in DNA repair. Xeroderma …
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Binding configurations of single-stranded DNA binding protein and their influence on DNA recombinase
DNA inside a cell is continuously damaged through multiple mechanisms including environmental exposure to radiation, chemical agents, or UV light. Certain products of the cell's own metabolism, such as reactive oxygen species, can also damage the DNA. In the worst-case scenario, this damage results …
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The evolutionary impacts of secondary structures within genomes of eukaryote-infecting single-stranded DNA viruses
… immune responses. Although computational DNA/RNA folding methods based-on free energy minimisation approaches are capable of predicting structures that form within virus genomes, these methods are not entirely accurate. Notably, many of structures that are accurately predicted will likely …
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CAPILLARY ELECTROPHORESIS ANALYSIS OF SINGLE-STRANDED DNA APTAMERS AND THEIR INTERACTIONS WITH PROTEIN TARGETS AND CARBON DOTS
Aptamers are short, single-stranded oligonucleotides, which fold into particular 3-dimensional structures permitting high-affinity binding to target molecules. In the first part of this work, capillary electrophoresis experiments were conducted to determine the dissociation constant of a unique …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
"One of the critical proteins involved in DNA replication across the three domains of life is single-stranded DNA-binding protein. At the replication fork, these proteins bind to stabilize and protect transiently exposed ssDNA from nucleases during DNA transactions. The ssDNA-binding protein is …
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Functional Analysis of Novel Replication Protein a Homologs in the Euryarchaeota: Implications in the Evolution of Single -Stranded DNA Binding Proteins Across the Three Domains of Life
Single-stranded DNA binding proteins (SSB) play an essential role in all cellular DNA transactions including DNA replication, repair, and recombination. At the replication fork, SSBs serve to stabilize and protect the transient intermediate ssDNA, which is generated by a replicative helicase. …
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W-reactivation (Inducible "SOS" DNA Repair) of double stranded DNA bacteriophage ? and single stranded DNA bacteriophage fd on isogenic rec and uvrB mutants of Escherichia coli K-12
… is remarkably deficient in inducible "SOS" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent enhancement of phage recovery. The recB-recF- double mutant like the recA mutant, is recombination deficient and …
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Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
… and expression of mutant proteins, all from a single vector. Chimeric phage-plasmid expression vectors, or phagemids, containing the intergenic region of the single stranded DNA bacteriophage f1 and either a bacteriophage SP6 or T7 promoter have been constructed. Coinfection of E. coli with …
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DNA detection system of homo-oligomers by electrochemical impedence measurements and quantification through radiolabeling
… dioxide electrodes with covalently bound single stranded homo-oligonucleotides was developed for DNA detection. Silanating agents (3-aminopropyltriethoxysilane, 3-glycodoxyethylpropylamine) were used to modify the electrodes and immobilize single stranded DNA. A method based on the …
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