Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"Single-particle tracking"”.
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Three-Dimensional Single Particle Tracking on the Two -Photon Microscope
We have developed a 3D single-particle-tracking (SPT) system based around the two-photon laser-scanning fluorescence microscope that can track particles in all three dimensions and at a high frequency response. We have implemented two different techniques to achieve this goal. Both techniques …
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Advances in volumetric super-resolution microscopy and single-particle tracking
Single-molecule localisation microscopy (SMLM) has enabled optical microscopy to probe biological structures and processes at the nanometer scale. To accommodate the inherently 3D nature of biological structures, 3D-SMLM techniques---such as the astigmatic and double-helix point spread functions …
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Single-particle tracking and fluorescence correlation spectroscopy for systems-level analysis of molecular dynamics in diverse biological systems
… levels. The ability to specifically label a single molecular species fluorescently has enabled the study of complex cellular structures through the visualization of their constituent components both individually as well as in context of the overall structure. Since the advent of engineered …
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Optimized Microbial Recombinant Production of HIV-1 Anti-Envelope Antibody Fragments with Applications to Single Particle Tracking of Virus Assembly
… cell surface during assembly of a mature virus particle. There also remains an insufficient amount of data for the understanding and clarification of the mechanism of action of a known host cell HIV-1 restriction factor, Serinc5 (SER 5), which is believed to have correlation with Env in fusion …
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X -Ray Diffraction Rotational Microrheology
Rotational single particle tracking microrheology can be used to find the storage and dissipative behavior of viscoelastic fluids of much higher viscosity than translational single particle tracking. The technique of X-ray diffraction rotational tracking has the ability to track the rotational …
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Spatial Stochastic Modeling of the ErbB Receptor Family
… with one another to trigger these cascades. Single Particle Tracking experiments have provided more precise and detailed measures of dimer lifetimes and diffusion. A major observation from the experiments is the anomalous diffusion of the receptors. One suggested contributor to this anomalous …
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Single particle motion of polymeric, colloidal, and biological materials
Small particles and their dynamics are of widespread interest due both to their unique properties and their ubiquity. Here, we investigate several classes of small particles: colloids, polymers, and liposomes. All these particles, due to their size on the order of microns, exhibit significant …
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On Protein Crystal Nucleation Precursors
… many times. Finally, we present an algorithm for single particle tracking to overcome challenges in image quality (low contrast, high particle density) and video frame rate. Although this algorithm was originally designed for quantifying the motility of breast cancer cells, single particle …
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Super-resolution imaging of glutamate receptors trafficking at live synapses
… previous studies were based on two-dimensional single particle tracking; lacking information on receptor movement in the z direction. Further more, the postsynapses were imaged with conventional fluorescent microscopy with diffraction-limited resolution. As a result, the detail localization of …
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Imaging the dynamics of soft materials at microscale and nanoscale
… and electron beam irradiation. Image processing, single particle tracking, and statistical analysis are combined to study the rich phenomena we observed.
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Multiscale Monte Carlo study of epidermal growth factor receptor diffusion and dimerization
… despite a plethora of biochemical studies and single particle tracking experiments, the early molecular mechanisms involving epidermal growth factor (EGF) binding and EGF receptor (EGFR) dimerization are not as well understood. Due to the large disparity of time and length scales involved in …
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Exploring the relation between the intracellular fate and biological activity of nucleic acid nanoparticles
… beeldvorming of het recent ontwikkelde Single Particle Tracking (SPT) die de intracellulaire processen in een volledige cel in beeld brengen. Er wordt verwacht dat de combinatie van conventionele microscopie met geavanceerde microscopie systemen zoals FCS en SPT, elk met hun specifieke …
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Anomalous diffusion of mRNA in the cytoplasm of HeLa cells
… with the HaloTag-JF646 fluorophore. We then used single-particle tracking to obtain trajectories of individual molecules. Trajectories were analyzed in terms of their mean-squared displacement (MSD) and power spectral density (PSD). We observed non-ergodic, subdiffusive behavior, with statistics …
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Analysis of Interphase Chromatin Motion in HeLa Cells
<p>Motion of particles under influencing forces may be observed under light microscopy techniques. Variations in mobility of particles may give relevant biophysical information. Automated high resolution single particle tracking techniques were used to characterize interphase chromatin mobility in …
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Polymer dynamics and fluctuations probed with fluorescence methods
… in a polymer solution, as experienced by a particle of comparable size is also investigated. These questions are fundamental in many scientific and technical applications, including adhesion, interdiffusion, friction, the mechanical behavior of composite and nanocomposite materials, and …
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Statistical imaging of transport in complex fluids: a journey from entangled polymers to living cells
Combining advanced fluorescence imaging, single particle tracking, and quantitative analysis in the framework of statistical mechanics, we studied several transport phenomena in complex fluids with nanometer and millisecond resolution. On the list are diffusion of nanoparticles and vesicles in …
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TWO-DIMENSIONAL FLUIDIZATION OF NANOMATERIALS VIA BIOMIMETIC MEMBRANES TOWARDS ASSISTED SELF-ASSEMBLY
… using wide-field fluorescent microscopy and single particle tracking techniques were employed to analyze their dynamic behavior. Next, an electric field was applied to the system to induce electroosmotic flow (EOF) which creates a bulk flow of the buffer solution. The quantum dots were again …
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Direct Single Molecule Imaging of Enhanced Diffusion for Enzymes and Enzyme-conjugated Dna origami
… into this process of enhanced diffusion using single-particle tracking (SPT) with total internal reflection fluorescence (TIRF) microscopy. We found that the mobility of individual enzymes was enhanced three-fold in the presence of the substrate, and the motion remained Brownian. We showed that …
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Serpin polymers enforce molecular filtration in the endoplasmic reticulum
… the stiffness of the resultant protein matrix. Single particle tracking of ER proteins revealed that this process initiates in cells with normal reticular ER morphology. This novel mechanism of ER dysfunction, involving phase transition of a protein in the ER lumen, provides a template for …
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A Biophysical Investigation of HIV-1 Envelope Cytoplasmic-Tail Mediated Retention in the Gag Lattice
… budding Gag lattice, where it is retained until particle scission and release. The mechanism of Env entry and retention in the lattice remains elusive and enigmatic. Here I aim to address the biophysical mechanisms that regulate the coalescence of these proteins through single-molecule and …
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