Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Single-particle Cryo-EM"”.
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2D image classification and alignment in single-particle Cryo-EM
Background and Objective: Single-particle Cryo-EM is a powerful tool for structural biologists to determine structures of macro-molecules at atomic resolution. In single-particle Cryo-EM, many individual biological molecular particles with identical structures are rapidly frozen at unknown, random …
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Affinity cryo-electron microscopy: Methods development and applications
<p>Single particle cryo-electron microscopy (cryo-EM) is an emerging powerful tool for structural studies of macromolecular assemblies. Although less concentrated and smaller amounts of samples are required for single particle cryo-EM compared to X-ray crystallography, it remains challenging to …
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Cryo-EM structural exploration of human VPS34 complexes and their activation by RAB GTPases and NRBF2
… into phosphatidylinositol-3-phosphate (PI3P) on membranes. The reaction product, PI3P, is a potent signalling lipid involved promoting autophagy, phagocytosis, and endocytic sorting in mammalian cells. In addition to its roles in normal homeostasis, VPS34 can be hijacked by viruses and bacteria …
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Machine Learning for Reconstructing Dynamic Protein Structures from Cryo-EM Images
… of dynamic biomolecular complexes via cryo-electron microscopy (cryo-EM). In single particle cryo-EM, the central problem is to reconstruct the 3D structure of a target biomolecular complex from a set of noisy and randomly oriented 2D projection images, a challenging inverse problem …
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Machine learning for cryo-EM structure determination
Electron cryo-microscopy (cryo-EM) has revolutionised structural biology thanks to its relaxed sample requirements. Recent advances in cryo-EM image processing have enabled the structural determination of many macromolecules that were previously intractable by other methods. At the same time, …
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Structural studies of the mitochondrial ribosome and co-translational insertion of membrane proteins
… has retained its own translational system to include mitochondrial ribosomes (mitoribosome) to translate these proteins along with other proteins which is species dependent. The mitoribosome is also involved in co-translational insertion of proteins destined for the inner mitochondrial …
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Structural Studies into the Mechanism and Organisation of Mammalian Respiratory Complex I
… proton pumping across the inner mitochondrial membrane, thus contributing to the proton motive force which drives ATP synthesis. With single particle cryo-EM as a routine approach for high-resolution structure determination, there are extensive complex I structures available across multiple …
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Crystal structure of the Large Type III Glutamine Synthetase from Bacteroides Fragilis
… Over the past five decades, extensive biochemical studies together with structural investigations have helped build a picture of the mechanism of functioning and regulation in the GSI and GSII families. The most divergent GSIII family, however, is poorly characterized and has only recently …
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The Influence of DNA Methylation on Both H2A.Z Nucleosome Dynamics and the Zinc Finger and Homeoboxes Transcription Factor Family
… as repressive ways, though exactly how a small chemical mark can enact such drastic and seemingly opposing effects on gene expression remains an open question. In my thesis work, I describe the influence DNA methylation has on two classes of chromatin proteins, the histone variant H2A.Z and the …
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Cryo-EM Studies of Bacterial RNA Polymerase
<p>In bacteria, a single RNA polymerase (RNAP) performs all transcription. The overall structure of bacterial RNAP resembles a crab claw with pincers comprising the β' and β subunits and a large cleft where the active site sits. Structural information about this essential enzyme has mainly been …
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Genome - Wide Regulatory Principles of Essential Transcription Factors from Mycobacterium Tuberculosis
… and antibiotics. Since bacteria only contain a single RNAP to express all genes, it is a prime therapeutic target. Most notably, the antibiotic rifampicin targets RNAP in the bacterial pathogen <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) and has therefore been a cornerstone of the …