Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Single-molecule techniques"”.
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Single-molecule techniques for mapping protein aggregates
… are heterogeneous in sizes and structures, single-molecule techniques are required to unravel their compositions. Super-resolution microscopy is particularly useful, as these aggregates are smaller than the diffraction limit of light. Compared to conventional light microscopy, the …
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Multi-domain Protein Unfolding Pathway Studies by Single Molecule Techniques
… way, the structural origin of the signal from single molecule force spectroscopy (SMFS) based on atomic force microscopy (AFM) can be directly identified. Beyond this published work, I also characterized and compared the unfolding pathway of two homologous multi-domain proteins – yeast …
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Single molecule techniques to probe decision-making processes in developmental biology
… that allow for quantitative, high-resolution, single-cell analysis that is robust but not associated with a high degree of technical difficulty or obscured by amplification. Here, we address these issues using two model systems, the developing mammalian inner ear and single mouse embryonic stem …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… activities in real-time, owing to the advent of single-molecule fluorescence- and force-based techniques that have enabled dynamic and quantitative measurements of protein-chromatin interactions central to nuclear biology. This thesis describes my work, in collaboration with others, at harnessing …
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Development of single molecule total internal reflection fluorescence microscope
We mostly think and communicate science at the single-molecule level, but do experiments at the ensemble level. This gap between our thinking and experiments has been closing with the advent of single-molecule techniques nearly twenty years ago. Single-molecule techniques have proved very useful in …
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Aggregation of alpha-synuclein using single-molecule spectroscopy
… conditions. In this thesis, well-established single-molecule techniques have been utilised to study the aggregation and oligomerisation of αS in solution.
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Single molecule study of weak and transient biological interactions
… breakthroughs were led in molecular biology with techniques allowing the study of dynamics at the resolution of an individual molecule. We have been expanding this capability by adapting porous vesicle encapsulation to single molecule fluorescence techniques. The porous vesicle encapsulation …
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Fluorescence-force spectroscopy at the single molecule level
During the past decade, various powerful single-molecule techniques have evolved and helped to address important questions in life sciences. As the single molecule techniques become mature, there is increasingly pressing need to maximize the information content of the analysis in order to be able …
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Single molecule studies of molecular electronics and biohybrid materials
… I directly address these challenges using single molecule techniques. Recent advances in single molecule techniques have ushered in a fundamentally new understanding of charge transport at the molecular level, which is a crucial step for designing new materials for molecular electronics and …
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Signal Processing In Single Molecule Studies
In this dissertation, I present signal processing techniques used in investigating single molecule kinetics and interactions. The first chapter provides an overview of signals and systems pertaining to single molecule studies. It discusses the need for signal processing, and gives a summary of …
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Investigating Poloidal Bias in DNA Minicircles and Overhang influence on DNA Dehybridization
DNA is a dynamic molecule that undergoes constant physical manipulation by proteins. For instance, it experiences extreme bending when packaged into nucleosomes and undergoes strand separation (dehybridization) during transcription. Single-molecule assays offer a powerful approach to uncover the …
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Advances in single molecule spectroscopy and microscopy for biological imaging and polymer characterization
Single molecule fluorescence microscopy and single molecule spectroscopy provide tremendously powerful methods for studying the behavior of a wide variety of biological systems. In this way, single molecule techniques can be used to gain an increased understanding of molecular mechanisms underlying …
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Theoretical investigation of protein search for targets on DNA
… 1D sliding of non-specifically bound protein molecules along the DNA chain. The recent development of single-molecule techniques has allowed for direct visualization of protein sliding during its binding site search. Thus, although we now have a better understanding of protein search on DNA, …
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Single-molecule studies of mechanical and helicase-catalyzed disruption of nucleic acid duplexes
… combining high-resolution optical tweezers with single-fluorophore sensitivity to observe the hybridization of ultrashort (<15 nt) DNA and RNA oligonucleotides under tension, one molecule at a time. We quantify the effect of tension on the rates of hybridization, and in doing so determine the …
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Dynamic imaging of nanoparticles on femtoseconds to minutes time scale at atomic resolution
… (STM) continues to be excavated to develop single-molecule techniques. In the last 20 years, our group has developed and improved the single-molecule absorption scanning tunneling microscopy (SMA--STM) technique that enabled high-resolution imaging of nanoparticles' electron probability …
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Single-molecule methods for an improved understanding of biophysical interactions: from fundamental biology to applied nanotechnology
The advent of single-molecule methods has greatly extended the scale at which we are able to probe natural systems. The information that can be gained by studying biological systems on a single-molecule scale, in the absence of ensemble averaging, provides an unprecedented amount of detail about …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
In the last 15 years, single molecule techniques have allowed us to observe the transient, heterogeneous, and multi‐step behavior of biomolecules often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism …
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Synthesis and supramolecular assembly of biomimetic polymers
… in materials chemistry is the synthesis of macromolecules and polymers with precise shapes and architectures. Polymer microstructure and architecture strongly affect the resulting functionality of advanced materials, yet understanding the static and dynamic properties of these complex …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
In the last 15 years, single molecule techniques have allowed us to observe the transient, heterogeneous, and multi‐step behavior of biomolecules often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism …
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Microfluidic diffusional sizing at the single molecule level
As the need for single-molecule techniques grows, commensurate with the desire to study complex samples and the heterogeneity of systems, this project presents the single-molecule microfluidic diffusional sizing (sm-MDS) platform and demonstrates its capabilities at performing diffusional sizing at …
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