Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 48 for “"Single-molecule studies"”.
-
Signal Processing In Single Molecule Studies
… processing techniques used in investigating single molecule kinetics and interactions. The first chapter provides an overview of signals and systems pertaining to single molecule studies. It discusses the need for signal processing, and gives a summary of current single molecule techniques. …
-
Single molecule studies of helicase mechanisms
… resolutions, as well as the ability to look at molecules one at a time instead of in an ensemble, have been shown to be helpful in elucidating the specific actions in many biological systems. Single molecule FRET (smFRET) microscopy provides these abilities with a distance scale of 2-8 nm, 8 ms …
-
Image Deconvolution Techniques for Single Molecule Studies
… is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological …
-
Single molecule studies of rna-target interactions
<p>We have used FRET, single molecule spectroscopy, and several other biophysical techniques to study how the folding of RNA allows it to perform its various functions by recognizing and binding a target ligand. We have shown that the c-di-GMP riboswitch undergoes a large, global conformational …
-
Single molecule studies of branched polymer dynamics
… properties for topologically complex polymers. Single molecule studies enable the direct observation of polymer chain dynamics at the molecular level; however, the vast majority of single polymer studies have only focused on linear DNA molecules (Chapter 2). In this dissertation, we extend …
-
Single molecule studies of flexible polymer systems
… of polymer solutions is not fully understood. Single molecule studies of polymer solutions provide an avenue for studying polymer dynamics and can aid in developing new molecular models of dynamic behavior. For nearly two decades, fluorescentlylabeled double stranded DNA (dsDNA) has been the …
-
Single-molecule studies of eukaryotic helicase loading
… investigation. In this thesis I describe a novel single-molecule helicase-loading assay that allows monitoring of protein associations and dissociations on a one-second time scale. By labeling pairs of helicase-loading proteins simultaneously, I determined the relative time of association and …
-
Single-Molecule Studies of CFTR Gating and Pharmacology
… I combined ensemble functional measurements, single-molecule fluorescence resonance energy transfer imaging, electrophysiology, electron microscopy and kinetic simulations to study human CFTR gating and conduction, as well as how these processes are altered by disease mutations and …
-
Single Molecule Studies Of Spliceosomal Snrnas U2-U6
… different activation states. We have used single-molecule fluorescence to probe the</p> <p>structural dynamics of a protein-free RNA complex modeling U2-U6 from yeast and</p> <p>mutants of highly conserved regions of U2-U6. Our data show the presence of at least</p> <p>three distinct …
-
Single-molecule studies of unconventional motor protein myosin VI
… presents the author‟s graduate work of several single-molecule studies on myosin VI. Special attention was paid to some of myosin VI‟s tail domains that consist of proximal tail (PT), medial tail (MT), distal tail (DT) domains and cargo-binding domain (CBD). The functional form of myosin VI in …
-
Single molecule studies of processes involved in genome maintenance
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01
-
Single molecule studies of molecular electronics and biohybrid materials
… I directly address these challenges using single molecule techniques. Recent advances in single molecule techniques have ushered in a fundamentally new understanding of charge transport at the molecular level, which is a crucial step for designing new materials for molecular electronics and …
-
Single-Molecule Studies Of Local And Global Nucleic-Acid Dynamics
… are important for their function. Structural studies have over the decades been invaluable in elucidation of various biomolecular mechanisms, hence contributing significantly to the understanding of biological events. However, a clear understanding of how molecules function in the cellular …
-
Noise in noise out - single molecule studies of molecular motors
… rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the …
-
Single-molecule studies of bacterial DNA replication and translesion synthesis
… presents the development of a co-localization single-molecule spectroscopy (CoSMoS) method to directly visualize the loading of the Escherichia coli replicative polymerase on DNA, as well as the exchange between the replicative polymerase and the translesion polymerases Pol II and Pol IV. In …
-
Single-molecule studies of the mechanism of eukaryotic helicase activation
… replication. In my thesis, I describe a novel single-molecule reaction that recapitulates helicase activation in vitro with purified proteins. This single-molecule method allows real-time monitoring of protein associations and dissociations during helicase activation. Through these …
-
Single-molecule studies of protein degradation and kinesin-8 motility
… Previous ensemble biochemical and structural studies uncovered many aspects of degradation activity of ClpXP but the mechanistic understanding of ClpXP function is currently still lacking. We employed single-molecule biophysical techniques including optical trapping and single-molecule …
-
Single molecule studies of ligand-DNA interactions using atomic force microscopy
… artefacts at picometer resolutions. In these studies, AFM imaging, supported by circular dichroism, reveals unusual conformational changes in DNA that occur as a result of the binding of ligands that incorporate the acridine chromophore. These changes are distinct from those observed following …
-
Single molecule studies of charge transport in organic redox-active materials
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-12-04 without embargo terms
Page 1 of 3