Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Single-molecule imaging"”.
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Single-molecule imaging studies of protein dynamics
Single-molecule fluorescence imaging is a powerful method for studying biological events. The work of this thesis primarily focuses on single molecule studies of the dynamics of Green Fluorescent Protein: GFP) and other fluorescent-labeled proteins by utilizing Total Internal Reflection …
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Polymer dynamics in crowded environment via single-molecule imaging
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Vortex Light Field Microscopy for 3D Spectral Single-Molecule Imaging
Singe-molecule localization microscopy (SMLM) is a powerful imaging technique that surpasses the diffraction limit and significantly improves spatial resolution over conventional light microscopy, allowing cellular structures and dynamic processes to be visualized at nanoscale. To solve the …
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Studying Models of the Amyloid Cascade Hypothesis Using Single-Molecule Imaging
… Aβ aggregates. Our group has been developing single-molecule and super- resolution microscopy techniques to characterise these aggregates. While these methods were previously applied to human samples, they have not been used to study disease models. Cellular and animal models have numerous …
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Transcription Factor Dynamics Investigated through Single-Molecule Imaging, High-Throughput Sequencing, and Neural Networks
The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.
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Direct Single Molecule Imaging of Enhanced Diffusion for Enzymes and Enzyme-conjugated Dna origami
… into this process of enhanced diffusion using single-particle tracking (SPT) with total internal reflection fluorescence (TIRF) microscopy. We found that the mobility of individual enzymes was enhanced three-fold in the presence of the substrate, and the motion remained Brownian. We showed that …
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Single Molecule Imaging of Human Immunodeficiency Virus Type-1 (HIV-1) Envelope Glycoprotein Dynamics Identifies Viral Elements Contributing to Infectious Particle Assembly
<p>The Human Immunodeficiency Virus Type-1 (HIV-1) has remained highly prevalent across the world for over 40 years, taking lives by attacking the immune system. There remains a lack of universal treatment options, which can be attributed to a poorly understood process in the life cycle of the …
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Towards understanding the mechanisms behind mRNA localisation in growing axons
… are generated. Here, I develop a method for live single molecule imaging of identified endogenous mRNA within Xenopus laevis retinal ganglion cell axons using molecular beacon technology coupled with highly inclined and laminated optical sheet (HILO) microscopy. By combining quantitative single …
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Single quantum dot tracking for quantitative molecular imaging
… the past few decades has advanced the field of single-molecule biology by allowing for long time-scale tracking of individual biomolecules. However, QD single-molecule imaging studies have almost been exclusively limited to the extracellular space, due to limitations in intracellular delivery …
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The role of adaptor protein Mal/TIRAP in transducing TLR signalling
… TNF-α and RANTES production). High resolution single molecule imaging techniques were developed and optimised to visualise Mal/TIRAP-Halo molecules in live and fixed cells, allowing the first physiological visualisation of Mal/TIRAP in immune cells. The single molecule imaging revealed critical …
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Direct real-time correlation of protein conformation and substrate recognition
… results. I developed a dual illumination, single-molecule imaging strategy to dissect directly and in real-time the correlation between domain motion of a DNA repair protein and its interaction with individual DNA substrates. The strategy was applied to XPD, an iron-sulfur (FeS) …
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Probing single-molecule dynamics of molecular motors and gene editing proteins
Single molecule fluorescence imaging is a powerful tool for studying protein dynamics. I have leveraged the power of single molecule imaging in studying two proteins systems: Molecular Motors and Gene Editing Proteins. Kinesin walks on cellular roads called microtubules and transports cellular …
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Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics
… years, e.g. by investigating the fluorescence of single molecules in biological cells, remarkable progress has been made in cell biology extending conventional ensemble techniques concerning temporal / spatial resolution and the detection of particle subpopulations [82]. In addition to employing …
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Development of a TIRF Microscope for Single Molecule Binding Studies
A single-molecule total internal reflection fluorescence microscope (TIRFM) is necessary to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal …
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Probing cellular protein complexes using single-molecule pull-down
… result from dynamic interactions between biomolecules. The gold standard method for investigating interaction between biomolecules is the pull-down assay. We have extended the conventional pull-down assay to its ultimate limit: the analysis of single biomolecular complexes. We achieve this …
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Super-resolution Microscopy: Novel Developments and Optimisations
… microscope at Cambridge Advanced Imaging Centre. The microscope is optimised to perform single and dual-colour imaging with high localisation precision and accuracy. Moreover, three-dimensional imaging capability is included in the microscope using the double-helix point spread …
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QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY
… describes our efforts in developing in-cell single-molecule imaging assays to quantify oligomeric states of membrane and cytosolic proteins. Quantifying oligomeric states of highly-expressed membrane proteins were achieved using protein localizations obtained from super-resolution microscopy. …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… probes for quantitative multicolour STORM imaging by Edward Taylor Current single molecule localisation microscopy methods allow for multicolour imaging of macromolecules in cells, and for a degree quantification on molecule numbers in one colour. However, that has not yet been an attempt …
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Molecular Mechanisms of Lymphocyte Activation - Please Mind the Gap
… phenomena in the light of the KS model. Single molecule and bulk experiments explore how the gap size between immune cells and a surface affects the behaviour of important signalling proteins. The first part of this thesis investigates whether the KS model explains the signalling-related …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
Recent advances in single-molecule fluorescence imaging techniques have allowed the direct observation of protein dynamics on DNA, but the progress has been largely limited to double-stranded DNA (dsDNA) or short single-stranded DNA (ssDNA). Here, a single molecule imaging approach for observing …
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