Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Single-molecule fluorescence imaging"”.
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Single-molecule imaging studies of protein dynamics
Single-molecule fluorescence imaging is a powerful method for studying biological events. The work of this thesis primarily focuses on single molecule studies of the dynamics of Green Fluorescent Protein: GFP) and other fluorescent-labeled proteins by utilizing Total Internal Reflection …
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Studies of Huntington's disease associated motor domain phosphorylation of kinesin-1
… kinesin transport, we used optical trapping and single-molecule fluorescence imaging to study purified kinesin. We found no significant difference in the processivity or ATPase activity of a phosphomimetic S175D construct or the non-phosphorylatable S175A construct. However, we did find that …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
Recent advances in single-molecule fluorescence imaging techniques have allowed the direct observation of protein dynamics on DNA, but the progress has been largely limited to double-stranded DNA (dsDNA) or short single-stranded DNA (ssDNA). Here, a single molecule imaging approach for observing …
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Development of Novel Molecular Imaging Contrast Agents for Detection of Oxidative Stress
… in cancer, the new method of photoacoustic imaging was exploited. A novel activatable, targeted near infrared cyanine dye is reported that enables specific detection of pathological levels of hydrogen peroxide, a major and abundant ROS in living organisms. This approach uses photoacoustic …
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Visualizing Mast Cell Activation: Single Molecule Dynamics of Early Events in FceRI Signaling
… signal transduction. In this work we employ single molecule fluorescence imaging techniques to quantify adapter protein recruitment, lateral mobility, receptor aggregation, and cytoskeletal organization to create a better understanding of many key processes in immune cell regulation. We focus …
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Single molecule investigation of transcription activator-like effector search dynamics
… sites. This work describes the application of single molecule fluorescence imaging to the study the TALE search process along specific and non-specific DNA templates. Our work provides a molecular-level picture of the dynamics of TALE-DNA interactions, and our results have revealed an …
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Advances in single molecule spectroscopy and microscopy for biological imaging and polymer characterization
Single molecule fluorescence microscopy and single molecule spectroscopy provide tremendously powerful methods for studying the behavior of a wide variety of biological systems. In this way, single molecule techniques can be used to gain an increased understanding of molecular mechanisms underlying …
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Probing single-molecule dynamics of molecular motors and gene editing proteins
Single molecule fluorescence imaging is a powerful tool for studying protein dynamics. I have leveraged the power of single molecule imaging in studying two proteins systems: Molecular Motors and Gene Editing Proteins. Kinesin walks on cellular roads called microtubules and transports cellular …
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Single Molecule Fluorescence Studies of Prions and Prion-like Proteins
… and Parkinson’s disease. In this thesis, I use single-aggregate imaging to study fibril fragmentation and elongation of individual murine PrP aggregates from seeded aggregation in vitro. From fluorescence imaging of individual PrP aggregates on the coverslip surface, elongation and fragmentation …
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Single molecule studies of polymers and self-assembling materials: Effects of chain topology and entanglements
… concentration regimes using a combination of single molecule fluorescence imaging and precise microfluidic flow manipulation under nonequilibrium flow conditions. This thesis also includes studies on the concentration-driven self-assembly and sol-gel transition of a new class of biohybrid …
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Single-molecule G-quadruplex visualisation in live cells and 3D chromatin
… fluorescent probes are reported which enable single-molecule real-time visualisation of G4 structures in living cells. Single-molecule imaging was performed at nanomolar probe concentrations providing measurements of relative populations of G4s in living cells without global perturbation of G4 …
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Photonic crystal enhanced fluorescence for single-molecule biosensing and early disease detection
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01
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Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics
Within the last years, e.g. by investigating the fluorescence of single molecules in biological cells, remarkable progress has been made in cell biology extending conventional ensemble techniques concerning temporal / spatial resolution and the detection of particle subpopulations [82]. In addition …