Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Single-molecule biophysics"”.
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Statistical Kinetics and Nonequilibrium Thermodynamics of Driven Systems: Stochastic Methods and Applications to Single-Molecule Biophysics
… the ability to obtain time traces of biomolecules at the single-molecule level of detail. These real-time trajectories provide details that are typically unavailable in ensemble-averaged experiments, such as the effect of conformational dynamics on enzymatic reactions. From a theoretical …
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Biology's wires and motors : single-molecule mechanics of M13 bacteriophage and kinesin
… and biology is strongly evident in the single-molecule regime, where high-resolution tools are addressing previously intractable biological problems, while nanotechnology is being accelerated by advances in molecular biology. This thesis explores both sides of the dichotomy in an effort …
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Quantum magnetic imaging for DNA biophysical measurements
An outstanding challenge in biophysics is sensing the relative bio-mechanical orienta-tion of single-molecule biological systems. Prior approaches used to study single-molecule biophysics rely on attaching fluorescent or otherwise optically detectable probes to bio- mechanical systems, and …
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Single-molecule methods for an improved understanding of biophysical interactions: from fundamental biology to applied nanotechnology
The advent of single-molecule methods has greatly extended the scale at which we are able to probe natural systems. The information that can be gained by studying biological systems on a single-molecule scale, in the absence of ensemble averaging, provides an unprecedented amount of detail about …
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Plasmonic nanoantennas for multipurpose particle manipulation and enhanced optical magnetism
… 1980s, it has since revolutionized the field of single-molecule biophysics, provided key insights into motor-protein function and DNA structure, and enabled the formation of the first Bose-Einstein condensates, and is the subject of significant current interest for lab-on-chip, colloidal physics, …
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NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY
… mediate the bidirectional trafficking of molecules between the nucleus and cytoplasm by forming a barrier. NE transmembrane proteins (NETs) embedded in either the outer nuclear membrane (ONM) or the inner nuclear membrane (INM) play crucial roles in both nuclear structure and functions, …
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Mechanisms of bacterial CRISPR immunity and a phage counter-defense
… and primed acquisition, using a high-throughput single-molecule assay (Chapter 2). We also detail a protocol for fluorescently tagging CRISPR-Cas protein subunits within a complex using a sortase-mediated labeling strategy (Chapter 3). Finally, we determine how the anti-CRISPR protein AcrIIA11 …